Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CHQ57_RS20570 Genome accession   NZ_CP022550
Coordinates   4400758..4402197 (-) Length   479 a.a.
NCBI ID   WP_099994508.1    Uniprot ID   A0AAX3VN16
Organism   Aeromonas salmonicida strain A527     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4395758..4407197
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CHQ57_RS20540 (CHQ57_20430) dagF 4396031..4396771 (+) 741 WP_099994506.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  CHQ57_RS20545 (CHQ57_20435) - 4396875..4398779 (+) 1905 WP_099994507.1 BglG family transcription antiterminator -
  CHQ57_RS20550 (CHQ57_20440) npr 4398798..4399070 (-) 273 WP_005314403.1 PTS phosphocarrier protein NPr -
  CHQ57_RS20555 (CHQ57_20445) rapZ 4399091..4399957 (-) 867 WP_005314405.1 RNase adapter RapZ -
  CHQ57_RS20560 (CHQ57_20450) ptsN 4400000..4400446 (-) 447 WP_005314406.1 PTS IIA-like nitrogen regulatory protein PtsN -
  CHQ57_RS20565 (CHQ57_20455) hpf 4400449..4400736 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  CHQ57_RS20570 (CHQ57_20460) rpoN/rpoN1 4400758..4402197 (-) 1440 WP_099994508.1 RNA polymerase factor sigma-54 Machinery gene
  CHQ57_RS20575 (CHQ57_20465) lptB 4402263..4402988 (-) 726 WP_005314412.1 LPS export ABC transporter ATP-binding protein -
  CHQ57_RS20580 (CHQ57_20470) lptA 4402992..4403519 (-) 528 WP_192873422.1 lipopolysaccharide transport periplasmic protein LptA -
  CHQ57_RS20585 (CHQ57_20475) lptC 4403506..4404066 (-) 561 WP_099994510.1 LPS export ABC transporter periplasmic protein LptC -
  CHQ57_RS20590 (CHQ57_20480) kdsC 4404063..4404617 (-) 555 WP_099994511.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  CHQ57_RS20595 (CHQ57_20485) - 4404617..4405612 (-) 996 WP_099994512.1 KpsF/GutQ family sugar-phosphate isomerase -
  CHQ57_RS20600 (CHQ57_20490) mlaF 4405819..4406619 (+) 801 WP_011898278.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53877.82 Da        Isoelectric Point: 4.3484

>NTDB_id=240638 CHQ57_RS20570 WP_099994508.1 4400758..4402197(-) (rpoN/rpoN1) [Aeromonas salmonicida strain A527]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEVQETSPEEPVDDSQLDSSDAMAQDQMP
DELPVDTTWEDVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDDSGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYAPELPWLNEAREVIKEHMDLLGNRDYRTLARK
TKLKESDLKEVLDLIQQLEPRPGNGIIQSDSQYVIPDVTVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVATCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=240638 CHQ57_RS20570 WP_099994508.1 4400758..4402197(-) (rpoN/rpoN1) [Aeromonas salmonicida strain A527]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAACAATCCTTTGCTTGAGCAAGAGGAGAATGAGG
TTCAGGAGACCAGCCCGGAAGAGCCCGTCGACGACAGTCAGCTCGACTCCAGCGATGCCATGGCCCAGGATCAGATGCCG
GATGAACTGCCGGTAGATACCACCTGGGAAGATGTCTATTCAGCCGGCACAGCCCAGAGCGCAGGCCCCATCCACGATGG
GGAGGACAGCGTCTATCAGGGGGAAACCACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCCTCACCCCGTTCA
GCGATCTGGATGCTGCCATTGCGCTGGCCATCATAGATGCCATCGATGACTCCGGTTATCTGACCGTCTCTCTCGAAGAT
ATACAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGACGAGGTCGAGGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATTGCCGCTCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTCGGCCAGTACGCTCCTGAACTGCCCT
GGCTCAATGAGGCCAGGGAGGTGATCAAGGAGCACATGGACTTGCTCGGCAACCGTGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTCAAGGAGAGTGACCTCAAGGAGGTGCTGGACCTTATCCAGCAGCTGGAGCCCAGGCCGGGTAACGGCATCAT
ACAGAGTGACTCCCAGTACGTGATCCCGGATGTCACCGTCGTTAAGAAGGGCAACAAATGGGTCGTCGAGCTCAACCCTG
ATTCTGCACCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGTCAGGCGCGTACCAGCCAGGATACCCAGTTC
ATTCGCTCCCACTTGCAAGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCGAC
CTGTATTGTCGAGCAACAACAGGGCTTTTTCGAGTATGGTGAAGAAGCCATGAAGCCCATGGTGCTCAACGATATCGCCG
AGGCGGTGGAGATGCATGAGTCGACCATTTCCAGGGTAACGACCCAGAAATACATGCATACCCCCCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTGAACACAGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTGATCAA
GAAGCTGGTGACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.972

100

0.489

Multiple sequence alignment