Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   C7M29_RS12880 Genome accession   NZ_CP028217
Coordinates   2487741..2488151 (+) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis strain SRCM102751     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2482741..2493151
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  C7M29_RS12855 (C7M29_02520) - 2482831..2483064 (-) 234 Protein_2468 helix-turn-helix domain-containing protein -
  C7M29_RS12860 (C7M29_02521) - 2483392..2484387 (-) 996 WP_128993175.1 acryloyl-CoA reductase -
  C7M29_RS12865 (C7M29_02522) - 2484545..2485138 (+) 594 WP_161476858.1 TetR/AcrR family transcriptional regulator -
  C7M29_RS20970 - 2485571..2485749 (+) 179 Protein_2471 hypothetical protein -
  C7M29_RS12870 (C7M29_02523) spoIVCA 2485707..2487167 (+) 1461 WP_250620476.1 site-specific DNA recombinase SpoIVCA -
  C7M29_RS12875 (C7M29_02524) - 2487198..2487545 (-) 348 Protein_2473 sigma-70 family RNA polymerase sigma factor -
  C7M29_RS12880 (C7M29_02525) nucA/comI 2487741..2488151 (+) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  C7M29_RS12885 (C7M29_02526) yqeB 2488184..2488906 (-) 723 WP_010886572.1 hypothetical protein -
  C7M29_RS12890 (C7M29_02527) gnd 2489158..2490051 (+) 894 WP_043857850.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  C7M29_RS12895 (C7M29_02528) yqeD 2490070..2490696 (-) 627 WP_161476859.1 TVP38/TMEM64 family protein -
  C7M29_RS12900 (C7M29_02529) cwlH 2490883..2491635 (+) 753 WP_128993171.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  C7M29_RS12905 (C7M29_02530) yqeF 2491887..2492618 (+) 732 WP_029317882.1 SGNH/GDSL hydrolase family protein -
  C7M29_RS12910 - 2492924..2493064 (-) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=240370 C7M29_RS12880 WP_009967785.1 2487741..2488151(+) (nucA/comI) [Bacillus subtilis strain SRCM102751]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=240370 C7M29_RS12880 WP_009967785.1 2487741..2488151(+) (nucA/comI) [Bacillus subtilis strain SRCM102751]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529