Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   O23A_RS15840 Genome accession   NZ_CP021654
Coordinates   3472068..3473507 (-) Length   479 a.a.
NCBI ID   WP_087756666.1    Uniprot ID   -
Organism   Aeromonas salmonicida strain O23A     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3467068..3478507
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O23A_RS15810 (O23A_p3269) dagF 3467340..3468080 (+) 741 WP_058394613.1 2-dehydro-3-deoxy-phosphogluconate aldolase -
  O23A_RS15815 (O23A_p3270) - 3468185..3470089 (+) 1905 WP_087756665.1 BglG family transcription antiterminator -
  O23A_RS15820 (O23A_p3271) npr 3470108..3470380 (-) 273 WP_005314403.1 PTS phosphocarrier protein NPr -
  O23A_RS15825 (O23A_p3272) rapZ 3470401..3471267 (-) 867 WP_005314405.1 RNase adapter RapZ -
  O23A_RS15830 (O23A_p3273) ptsN 3471310..3471756 (-) 447 WP_005314406.1 PTS IIA-like nitrogen regulatory protein PtsN -
  O23A_RS15835 (O23A_p3274) hpf 3471759..3472046 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  O23A_RS15840 (O23A_p3275) rpoN/rpoN1 3472068..3473507 (-) 1440 WP_087756666.1 RNA polymerase factor sigma-54 Machinery gene
  O23A_RS15845 (O23A_p3276) lptB 3473573..3474298 (-) 726 WP_005314412.1 LPS export ABC transporter ATP-binding protein -
  O23A_RS15850 (O23A_p3277) lptA 3474302..3474829 (-) 528 WP_005314414.1 lipopolysaccharide transport periplasmic protein LptA -
  O23A_RS15855 (O23A_p3278) lptC 3474816..3475376 (-) 561 WP_021140208.1 LPS export ABC transporter periplasmic protein LptC -
  O23A_RS15860 (O23A_p3279) kdsC 3475373..3475927 (-) 555 WP_005314419.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  O23A_RS15865 (O23A_p3280) - 3475927..3476922 (-) 996 WP_034524103.1 KpsF/GutQ family sugar-phosphate isomerase -
  O23A_RS15870 (O23A_p3281) mlaF 3477130..3477930 (+) 801 WP_011898278.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53907.84 Da        Isoelectric Point: 4.3484

>NTDB_id=232300 O23A_RS15840 WP_087756666.1 3472068..3473507(-) (rpoN/rpoN1) [Aeromonas salmonicida strain O23A]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEVQETSPEEPVDDSQLDSSDAMAQDQMP
DELPVDTTWEDVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDDSGYLTVSLED
IQAAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYAPELPWLNEAREVIKEHMDLLGNRDYRTLARK
TKLKESDLKEVLDLIQQLEPRPGNGIIQSDSQYVIPDVTVVKKSNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVATCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=232300 O23A_RS15840 WP_087756666.1 3472068..3473507(-) (rpoN/rpoN1) [Aeromonas salmonicida strain O23A]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAACAATCCTTTGCTTGAGCAAGAGGAGAATGAGG
TTCAGGAGACCAGCCCGGAAGAGCCCGTCGACGACAGTCAGCTCGACTCCAGTGATGCCATGGCCCAGGATCAGATGCCG
GACGAACTGCCGGTAGATACCACCTGGGAAGATGTCTATTCAGCCGGCACAGCCCAGAGCGCAGGCCCCATCCACGATGG
GGAGGACAGCGTCTATCAGGGGGAAACCACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCCTCACCCCGTTCA
GCGATCTGGATGCGGCCATTGCGCTGGCCATCATAGATGCCATCGATGACTCCGGTTATCTGACCGTCTCTCTCGAAGAT
ATACAGGCGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGACGAGGTCGAGGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTTGGCCAGTACGCTCCTGAACTGCCCT
GGCTCAATGAGGCCAGGGAGGTGATCAAGGAGCACATGGATCTGCTCGGCAACCGTGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTCAAGGAGAGTGACCTCAAGGAGGTGCTGGACCTTATCCAGCAACTGGAGCCCAGGCCGGGTAACGGTATCAT
ACAGAGTGACTCCCAGTACGTGATCCCGGATGTCACCGTCGTCAAGAAGAGCAACAAATGGGTCGTCGAACTCAACCCGG
ATTCTGCACCGCGCATCCGGGTCAACGAGACCTATGCCGCCATGGCCCGTCAGGCACGTACCAGCCAGGATACCCAGTTC
ATTCGCTCCCACTTGCAAGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCGAC
CTGTATTGTCGAGCAACAACAGGGCTTTTTCGAGTATGGTGAAGAAGCCATGAAGCCCATGGTGCTCAACGATATTGCCG
AGGCGGTGGAGATGCATGAGTCGACCATTTCCAGGGTAACGACCCAGAAATACATGCATACCCCCCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGTCATGTGAACACAGAAGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTGATCAA
GAAGCTGGTGACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterProScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.972

100

0.489

Multiple sequence alignment