Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   CAL14_RS00325 Genome accession   NZ_CP021110
Coordinates   72917..74356 (+) Length   479 a.a.
NCBI ID   WP_086055731.1    Uniprot ID   -
Organism   Bordetella genomosp. 9 strain AU14267     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 67917..79356
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAL14_RS00305 (CAL14_00305) - 68273..69136 (+) 864 WP_420042594.1 carbohydrate ABC transporter permease -
  CAL14_RS00310 (CAL14_00310) - 69133..70023 (+) 891 WP_086055728.1 carbohydrate ABC transporter permease -
  CAL14_RS00315 (CAL14_00315) leuA 70096..71805 (-) 1710 WP_086055729.1 2-isopropylmalate synthase -
  CAL14_RS00320 (CAL14_00320) - 72187..72918 (-) 732 WP_086055730.1 hypothetical protein -
  CAL14_RS00325 (CAL14_00325) rpoN/rpoN1 72917..74356 (+) 1440 WP_086055731.1 RNA polymerase factor sigma-54 Machinery gene
  CAL14_RS00330 (CAL14_00330) - 74460..74732 (+) 273 WP_086055732.1 (2Fe-2S)-binding protein -
  CAL14_RS00335 (CAL14_00335) - 75098..76015 (+) 918 WP_086055733.1 DMT family transporter -
  CAL14_RS00340 (CAL14_00340) bfr 76213..76689 (+) 477 WP_086055734.1 bacterioferritin -
  CAL14_RS00345 (CAL14_00345) - 76808..77497 (-) 690 WP_086055735.1 hypothetical protein -
  CAL14_RS00350 (CAL14_00350) - 77690..79297 (-) 1608 WP_086055736.1 peptide chain release factor 3 -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 52652.10 Da        Isoelectric Point: 5.3886

>NTDB_id=228293 CAL14_RS00325 WP_086055731.1 72917..74356(+) (rpoN/rpoN1) [Bordetella genomosp. 9 strain AU14267]
MQNIVPMTRHALELRPGQHLALTPQLQQSIRMLQLSTLDLEAEISQALAENPLLERDDNPAPDDIDANREGSAHEDEPPP
ERETPVDEMPGAKGVYADEDGDMPEAARGETLREHLLAQLALTRAGRRDAALVALLIDELDENGYLGSPLEEILAWLPAE
VEADLDELRAALRLLQSFDPPGIGARDMAECLALQLRHPDVEALPEAADPTVLACARAICAEHLPLLASGNQARLREALL
CDDATARRARALILKLDPRPGRRWTVPAADYAIPDVIVRKSRKGWQVVLNSAAMPRLRVNHIYAQMLGNQREGGHSALHA
QLQQARWMIRNVEQRFDTILRVAQAIVDHQSAFFSQGPSAMRPLILKDIAGELGLHESTISRATTQKYMLTPFGTMELKD
FFGAGVATESGDTASATAVQAFIKRLVAEENRAKPLSDNQIMLKLAEQGIVIARRTVAKYREALRIAPASVRKAQASAR

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=228293 CAL14_RS00325 WP_086055731.1 72917..74356(+) (rpoN/rpoN1) [Bordetella genomosp. 9 strain AU14267]
ATGCAAAATATTGTACCTATGACTCGCCACGCCTTAGAACTTCGGCCAGGACAGCACCTCGCGCTGACCCCGCAGCTGCA
GCAATCCATCCGGATGCTGCAGTTGTCGACGCTCGACCTGGAGGCGGAGATTTCCCAGGCGCTGGCCGAAAACCCCTTGC
TGGAGCGCGACGACAACCCCGCGCCGGACGATATCGACGCCAATCGCGAGGGGTCGGCGCACGAGGACGAGCCGCCGCCG
GAAAGGGAAACCCCGGTGGACGAAATGCCCGGCGCCAAGGGCGTGTACGCGGACGAGGACGGCGACATGCCGGAAGCCGC
GCGGGGCGAAACCCTGCGGGAACACCTGCTGGCGCAATTGGCGCTGACGCGCGCGGGCCGGCGCGACGCCGCTCTGGTGG
CGCTGCTGATCGACGAGCTGGACGAAAACGGCTACCTGGGTTCGCCCCTGGAGGAAATCCTGGCCTGGCTGCCTGCCGAG
GTGGAAGCGGACCTGGACGAGTTGCGCGCCGCCTTGCGCCTGCTGCAGTCCTTCGACCCGCCTGGTATCGGGGCGCGCGA
CATGGCCGAATGCCTGGCGCTGCAGCTGCGGCACCCCGACGTCGAGGCGCTGCCAGAGGCTGCGGATCCGACCGTACTGG
CCTGCGCGCGCGCCATCTGCGCCGAACATCTGCCGTTGCTCGCATCGGGCAATCAGGCCCGGCTGCGGGAGGCGTTGCTT
TGCGACGACGCCACCGCCCGGCGCGCTCGCGCCCTGATCCTGAAGCTGGACCCGCGCCCCGGCCGCCGCTGGACCGTGCC
GGCGGCCGACTATGCCATTCCGGACGTCATCGTCCGCAAGAGCCGCAAGGGGTGGCAGGTCGTGTTGAACAGCGCCGCGA
TGCCGCGCCTGCGCGTCAACCACATCTACGCTCAGATGCTCGGCAATCAGCGGGAAGGCGGGCATTCGGCCCTGCACGCG
CAATTGCAGCAGGCGCGCTGGATGATCCGCAATGTCGAGCAGCGCTTCGATACCATCCTGCGGGTTGCCCAGGCCATCGT
CGACCATCAGTCGGCTTTCTTCAGCCAGGGCCCGTCCGCGATGCGGCCCCTCATCCTCAAGGACATCGCAGGTGAGTTGG
GGCTGCACGAGTCTACGATTTCGCGGGCCACCACACAGAAGTACATGCTTACACCGTTCGGCACCATGGAGCTGAAGGAC
TTTTTCGGCGCCGGCGTTGCCACGGAAAGCGGGGACACGGCCTCCGCGACGGCGGTCCAGGCTTTCATCAAACGGCTCGT
GGCGGAAGAAAACCGTGCCAAGCCGCTATCCGACAATCAAATCATGCTCAAGCTGGCGGAGCAGGGCATCGTCATCGCGC
GCCGCACCGTGGCGAAGTATCGTGAGGCGCTGCGTATCGCACCGGCCTCTGTCCGCAAGGCGCAGGCGTCCGCGCGATAG

Domains

Predicted by InterProScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-473)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(12-55)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(106-303)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

49.209

100

0.52

Multiple sequence alignment