Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   ECA_RS18220 Genome accession   NC_004547
Coordinates   4140147..4140710 (+) Length   187 a.a.
NCBI ID   WP_011095190.1    Uniprot ID   -
Organism   Pectobacterium atrosepticum SCRI1043     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 4140147..4186931 4140147..4140710 within 0


Gene organization within MGE regions


Location: 4140147..4186931
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ECA_RS18220 (ECA3690) ssb 4140147..4140710 (+) 564 WP_011095190.1 single-stranded DNA-binding protein Machinery gene
  ECA_RS18225 (ECA3691) - 4140956..4142812 (+) 1857 WP_039293548.1 amidohydrolase -
  ECA_RS18230 (ECA3692) - 4143007..4143462 (+) 456 WP_011095192.1 GNAT family N-acetyltransferase -
  ECA_RS18235 (ECA3693) - 4143714..4144145 (-) 432 WP_011095193.1 carboxymuconolactone decarboxylase family protein -
  ECA_RS18240 (ECA3694) - 4144286..4144435 (-) 150 Protein_3643 GNAT family N-acetyltransferase -
  ECA_RS18245 (ECA3695) - 4144591..4144983 (-) 393 WP_011095195.1 Mor transcription activator family protein -
  ECA_RS18250 (ECA3696) - 4144980..4145402 (-) 423 WP_011095196.1 gp16 family protein -
  ECA_RS18255 (ECA3697) - 4145380..4145580 (-) 201 WP_011095197.1 hypothetical protein -
  ECA_RS18260 (ECA3698) - 4145577..4146017 (-) 441 WP_011095198.1 hypothetical protein -
  ECA_RS18265 (ECA3699) - 4146028..4146327 (-) 300 WP_011095199.1 hypothetical protein -
  ECA_RS18270 (ECA3700) - 4146418..4147002 (-) 585 WP_039295283.1 DUF3164 family protein -
  ECA_RS18275 (ECA3701) - 4147039..4147308 (-) 270 WP_011095201.1 hypothetical protein -
  ECA_RS18280 (ECA3702) - 4147336..4147593 (-) 258 WP_011095202.1 phage-like protein -
  ECA_RS18285 (ECA3703) - 4147596..4148747 (-) 1152 WP_011095203.1 ExeA family protein -
  ECA_RS18290 (ECA3704) - 4148757..4150526 (-) 1770 WP_011095204.1 DDE-type integrase/transposase/recombinase -
  ECA_RS18295 (ECA3705) - 4150536..4151444 (-) 909 WP_011095205.1 DUF3102 domain-containing protein -
  ECA_RS18300 (ECA3706) - 4151454..4151759 (-) 306 WP_011095206.1 helix-turn-helix domain-containing protein -
  ECA_RS18305 (ECA3707) - 4151812..4152000 (-) 189 WP_011095207.1 DNA-binding protein -
  ECA_RS23170 (ECA3708) - 4152092..4152508 (+) 417 WP_011095208.1 helix-turn-helix domain-containing protein -
  ECA_RS18315 (ECA3709) - 4152527..4153063 (+) 537 WP_011095209.1 hypothetical protein -
  ECA_RS18320 (ECA3710) - 4153098..4154042 (+) 945 WP_011095210.1 hypothetical protein -
  ECA_RS18325 (ECA3711) - 4154162..4155169 (+) 1008 WP_011095211.1 nucleoid-associated protein -
  ECA_RS18330 (ECA3712) - 4155172..4156353 (+) 1182 WP_011095212.1 membrane protein -
  ECA_RS18335 (ECA3713) - 4156501..4156845 (+) 345 WP_011095213.1 putative holin -
  ECA_RS18340 (ECA3714) - 4156848..4157495 (+) 648 WP_011095214.1 transglycosylase SLT domain-containing protein -
  ECA_RS18345 (ECA3715) - 4157479..4158150 (+) 672 WP_011095215.1 hypothetical protein -
  ECA_RS18350 (ECA3716) - 4158150..4158482 (+) 333 WP_011095216.1 hypothetical protein -
  ECA_RS18355 (ECA3717) - 4158475..4158786 (+) 312 WP_011095217.1 hypothetical protein -
  ECA_RS18360 (ECA3718) - 4158788..4159333 (+) 546 WP_011095218.1 DUF3486 family protein -
  ECA_RS18365 (ECA3719) - 4159330..4160865 (+) 1536 WP_011095219.1 hypothetical protein -
  ECA_RS18370 (ECA3720) - 4160865..4162358 (+) 1494 WP_011095220.1 DUF935 domain-containing protein -
  ECA_RS18375 (ECA3721) - 4162339..4163160 (+) 822 WP_011095221.1 phage minor head protein -
  ECA_RS18380 (ECA3722) - 4163157..4163606 (+) 450 WP_011095222.1 phage virion morphogenesis protein -
  ECA_RS18385 (ECA3723) - 4163802..4164911 (+) 1110 WP_011095223.1 peptidase -
  ECA_RS18390 (ECA3724) - 4164948..4165883 (+) 936 WP_011095224.1 hypothetical protein -
  ECA_RS18395 (ECA3725) - 4165894..4166232 (+) 339 WP_011095225.1 capsid cement protein -
  ECA_RS18400 (ECA3726) - 4166235..4166681 (+) 447 WP_011095226.1 gp436 family protein -
  ECA_RS18405 (ECA3727) - 4166681..4167148 (+) 468 WP_011095227.1 Gp37 family protein -
  ECA_RS18410 (ECA3728) - 4167142..4167369 (+) 228 WP_173361635.1 hypothetical protein -
  ECA_RS18415 (ECA3729) - 4167359..4168786 (+) 1428 WP_011095229.1 phage tail sheath subtilisin-like domain-containing protein -
  ECA_RS18420 (ECA3730) - 4168786..4169310 (+) 525 WP_011095230.1 phage major tail tube protein -
  ECA_RS18425 (ECA3731) - 4169317..4169517 (+) 201 WP_011095231.1 hypothetical protein -
  ECA_RS18430 (ECA3731A) - 4169658..4169960 (+) 303 WP_011095232.1 phage tail assembly protein -
  ECA_RS18435 - 4170035..4170304 (-) 270 WP_039293937.1 hypothetical protein -
  ECA_RS18440 (ECA3732) - 4170347..4172818 (+) 2472 WP_011095233.1 phage tail tape measure protein -
  ECA_RS18445 (ECA3733) - 4172818..4173702 (+) 885 WP_011095234.1 phage tail protein -
  ECA_RS18450 (ECA3734) - 4173702..4173914 (+) 213 WP_103791629.1 tail protein X -
  ECA_RS18455 (ECA3735) - 4173902..4175047 (+) 1146 WP_011095236.1 phage late control D family protein -
  ECA_RS18460 (ECA3736) - 4175044..4175637 (+) 594 WP_011095237.1 phage baseplate assembly protein V -
  ECA_RS18465 (ECA3737) - 4175664..4176512 (+) 849 WP_011095238.1 AbiJ-NTD4 domain-containing protein -
  ECA_RS18470 (ECA3738) - 4176578..4176925 (+) 348 WP_011095239.1 GPW/gp25 family protein -
  ECA_RS18475 (ECA3739) - 4176916..4178022 (+) 1107 WP_011095240.1 baseplate assembly protein -
  ECA_RS18480 (ECA3740) - 4178015..4178590 (+) 576 WP_011095241.1 phage tail protein -
  ECA_RS18485 (ECA3741) - 4178591..4180153 (+) 1563 WP_011095242.1 phage tail protein -
  ECA_RS18490 (ECA3742) - 4180153..4180770 (+) 618 WP_011095243.1 tail fiber assembly protein -
  ECA_RS18495 (ECA3743) - 4180822..4181157 (-) 336 WP_011095244.1 hypothetical protein -
  ECA_RS18500 (ECA3744) - 4181249..4182667 (+) 1419 WP_011095245.1 PLP-dependent aminotransferase family protein -
  ECA_RS18505 (ECA3745) - 4182708..4183757 (-) 1050 WP_011095246.1 LacI family DNA-binding transcriptional regulator -
  ECA_RS18510 (ECA3746) - 4183757..4184809 (-) 1053 WP_011095247.1 carbohydrate ABC transporter permease -
  ECA_RS18515 (ECA3747) - 4184818..4185816 (-) 999 WP_011095248.1 carbohydrate ABC transporter permease -
  ECA_RS18520 (ECA3748) - 4185840..4186931 (-) 1092 WP_011095249.1 ABC transporter ATP-binding protein -

Sequence


Protein


Download         Length: 187 a.a.        Molecular weight: 19726.69 Da        Isoelectric Point: 5.2456

>NTDB_id=22628 ECA_RS18220 WP_011095190.1 4140147..4140710(+) (ssb) [Pectobacterium atrosepticum SCRI1043]
MASRGVNKVILVGNLGQDPEVRYMPNGGAVANITLATSESWRDKQTGEQKEKTEWHRVVLFGKLAEVAGEYLRKGSQVYI
EGALQTRKWADQAGVERYTTEVVVNVGGTMQMLGGRQGGGAPAGGGNAGGGQPQGGWGQPQQPQGGNQFSGGAQAQQRPA
QNASSSQNNAPAQSNEPPMDFDDDIPF

Nucleotide


Download         Length: 564 bp        

>NTDB_id=22628 ECA_RS18220 WP_011095190.1 4140147..4140710(+) (ssb) [Pectobacterium atrosepticum SCRI1043]
ATGGCCAGCAGAGGCGTTAATAAAGTGATTCTTGTCGGGAATCTGGGTCAAGACCCAGAAGTCCGCTATATGCCGAATGG
TGGTGCAGTTGCCAACATCACGCTGGCTACGTCGGAAAGCTGGCGTGACAAGCAAACCGGTGAGCAGAAAGAGAAGACCG
AATGGCACCGTGTGGTTCTGTTTGGCAAGCTGGCAGAAGTCGCGGGCGAATACCTGCGTAAAGGCTCTCAGGTCTACATC
GAAGGCGCACTGCAAACCCGTAAATGGGCCGATCAGGCTGGCGTAGAGCGCTACACCACCGAAGTCGTCGTTAACGTCGG
CGGCACCATGCAGATGCTAGGTGGACGTCAGGGCGGCGGCGCACCAGCAGGCGGTGGCAATGCAGGTGGCGGTCAGCCAC
AAGGCGGCTGGGGTCAACCTCAGCAACCGCAGGGCGGAAACCAGTTCAGCGGCGGCGCACAAGCTCAGCAGCGCCCAGCT
CAGAACGCATCTTCCTCACAAAATAATGCCCCGGCACAAAGCAACGAACCACCAATGGATTTCGACGACGATATTCCTTT
CTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Vibrio cholerae strain A1552

71.429

100

0.722

  ssb Glaesserella parasuis strain SC1401

55.208

100

0.567

  ssb Neisseria meningitidis MC58

44.974

100

0.455

  ssb Neisseria gonorrhoeae MS11

44.974

100

0.455

  ssbA Bacillus subtilis subsp. subtilis str. 168

37.158

97.861

0.364


Multiple sequence alignment