Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   B5S57_RS07520 Genome accession   NZ_CP020534
Coordinates   420771..422486 (+) Length   571 a.a.
NCBI ID   WP_013855889.1    Uniprot ID   A0AAW8GVH2
Organism   Vibrio anguillarum strain 425     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 415771..427486
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B5S57_RS07500 (B5S57_07390) pilM 418051..419061 (+) 1011 WP_013855885.1 type IV pilus assembly protein PilM Machinery gene
  B5S57_RS07505 (B5S57_07395) pilN 419051..419653 (+) 603 WP_013855886.1 PilN domain-containing protein Machinery gene
  B5S57_RS07510 (B5S57_07400) pilO 419646..420239 (+) 594 WP_013855887.1 type 4a pilus biogenesis protein PilO Machinery gene
  B5S57_RS07515 (B5S57_07405) pilP 420226..420747 (+) 522 WP_013855888.1 pilus assembly protein PilP Machinery gene
  B5S57_RS07520 (B5S57_07410) pilQ 420771..422486 (+) 1716 WP_013855889.1 type IV pilus secretin PilQ Machinery gene
  B5S57_RS07525 (B5S57_07415) aroK 422673..423194 (+) 522 WP_010317691.1 shikimate kinase AroK -
  B5S57_RS07530 (B5S57_07420) aroB 423219..424307 (+) 1089 WP_026027894.1 3-dehydroquinate synthase -
  B5S57_RS07535 (B5S57_07425) - 424356..425855 (+) 1500 WP_013855891.1 SPOR domain-containing protein -
  B5S57_RS07540 (B5S57_07430) - 425925..426755 (+) 831 WP_013855892.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62304.79 Da        Isoelectric Point: 4.8701

>NTDB_id=223946 B5S57_RS07520 WP_013855889.1 420771..422486(+) (pilQ) [Vibrio anguillarum strain 425]
MIKGLQTQMTMVFRSLLFGLLLSVAGLSVAAETTQTNELKGIDFRVNKNKDAVLIVELASPSTVVNVQKVADGLQIELLK
TDVSNEKLYVLDVKDFATVVESIEVLRKEPSTQLLATIHGEFKYDYSLKGKYLEVVIGKLKEQEKPKPKSILEKEGKLIS
INFQDIPVRNVLQLIADYNAFNLVVSDSVTGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPKEELDLREKQQL
EKARLAEELGDLRSEIIKINFAKASDIAQMIGGEGAVSMLSERGSISIDERTNSLLIRELAENIVVIRQIVESLDIPVKQ
VQIEARIVTVNEGNLDELGVRWGFTSTNGNNTAGGSIESNLFQVGKLGESTLPVDDFLNVNLAATSANASSIAFQVAKLG
SDMLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVAFKKAVLSLKVTPQITPDNRLVLDL
SVTQDRPGDIVKTGTGEAVAINTQRIGTQVLVNNGETVVLGGIFQHSVNNTVDKVPLLGDLPLLGALFRRSYEQVGKSEL
LIFVTPKVVIQ

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=223946 B5S57_RS07520 WP_013855889.1 420771..422486(+) (pilQ) [Vibrio anguillarum strain 425]
ATGATTAAAGGACTACAGACACAAATGACTATGGTATTTCGGAGCCTCTTATTTGGCTTATTATTGAGTGTGGCAGGGCT
AAGTGTGGCGGCAGAAACGACGCAGACCAACGAGCTTAAAGGCATTGATTTTCGAGTCAATAAAAACAAAGACGCTGTAT
TGATTGTTGAACTCGCATCACCTTCGACAGTAGTTAATGTGCAGAAAGTGGCTGACGGTTTACAAATTGAACTGCTTAAG
ACGGATGTCTCCAATGAGAAACTGTATGTCTTGGACGTCAAAGATTTTGCAACCGTTGTTGAGAGTATCGAGGTATTGCG
CAAAGAGCCAAGTACACAGTTACTGGCGACCATTCATGGCGAGTTTAAGTATGATTACAGCTTGAAAGGGAAATACCTTG
AGGTGGTGATTGGCAAACTGAAAGAACAAGAAAAACCTAAGCCGAAGAGCATTCTTGAGAAAGAGGGCAAGCTTATCTCG
ATTAACTTTCAAGATATTCCTGTGCGAAATGTTTTGCAGCTTATCGCAGATTATAACGCGTTTAACCTAGTCGTCTCAGA
TTCGGTCACTGGTAACCTGACGCTACGGCTTGATGGCGTGCCTTGGCAGCAAGTGCTCGATATCATTTTGCAAGTCAAGG
GATTAGATAAGCGTGTTGACGGCAATGTGATTTTAGTTGCACCTAAAGAAGAGTTGGATTTACGTGAAAAGCAGCAGCTT
GAAAAGGCACGTTTGGCTGAAGAGTTGGGCGATCTACGTTCTGAAATCATCAAAATTAATTTTGCTAAGGCGTCTGATAT
TGCGCAGATGATTGGCGGTGAAGGGGCGGTCAGCATGTTGTCTGAGCGAGGTTCCATCAGTATCGACGAGCGAACCAACT
CACTGCTGATCCGTGAATTGGCTGAGAATATTGTGGTGATACGCCAAATTGTTGAATCGCTCGATATCCCGGTCAAACAG
GTACAGATTGAAGCGCGCATTGTTACGGTCAACGAAGGTAATTTGGATGAACTTGGCGTACGCTGGGGTTTCACCTCGAC
CAATGGCAACAACACCGCAGGCGGAAGTATTGAAAGTAATTTATTCCAAGTGGGCAAGTTGGGGGAAAGCACATTGCCCG
TTGATGACTTCCTTAACGTAAACTTAGCGGCGACCTCCGCTAATGCCTCAAGTATTGCCTTCCAAGTGGCTAAGTTGGGG
TCGGATATGTTGTTGGATTTAGAGCTTTCAGCGCTACAACGTGAGTCGAAAGCGGAGATCATTTCCAGCCCTCGTTTGAT
TACGACCAACAAGAAACCCGCTTATATCGAGCAGGGTACGGAGATCCCTTATTTGGAGTCTTCTTCCAGCGGGGCAACGA
CAGTCGCCTTCAAAAAAGCGGTGCTGAGTTTAAAAGTGACGCCACAGATAACGCCGGATAATCGTTTAGTGTTAGATCTC
AGTGTCACTCAAGACCGGCCTGGGGATATTGTAAAAACAGGGACGGGAGAAGCGGTTGCAATTAATACTCAGCGTATTGG
CACTCAAGTATTGGTCAATAATGGAGAAACCGTGGTCCTTGGTGGAATATTCCAACATAGCGTCAACAATACGGTGGATA
AGGTGCCATTGCTTGGTGATTTGCCATTACTTGGTGCATTGTTCCGCCGTTCCTATGAGCAAGTGGGCAAAAGTGAGCTG
CTGATTTTCGTGACACCGAAAGTCGTCATTCAATAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(255-320)

Bacterial type II and III secretion system protein (Secretin)

(411-569)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

82.312

100

0.823
  pilQ Vibrio cholerae strain A1552

82.312

100

0.823
  pilQ Vibrio campbellii strain DS40M4

79.129

96.497

0.764
  pilQ Legionella pneumophila strain ERS1305867

42.222

94.571

0.399
  pilQ Legionella pneumophila strain Lp02

42.222

94.571

0.399
  pilQ Pseudomonas aeruginosa PAK

39.855

96.673

0.385
  comQ Acinetobacter baylyi ADP1

39.247

97.723

0.384
  pilQ Acinetobacter baumannii strain A118

36.489

100

0.371
  pilQ Acinetobacter baumannii D1279779

36.489

100

0.371

Multiple sequence alignment