Detailed information NTG0218412

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: B0D95_RS01295
• Genome accession: NZ_CP019799
• Coordinates: 279549..280724 (+)
• Length: 391 a.a.
• NCBI ID: WP_078042195.1
• Uniprot ID: A0A1U9N5I8
• Organism: Cellvibrio sp. PSBB023
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 274549..285724

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B0D95_RS01275 (B0D95_01275)	-	275799..277004 (-)	1206	WP_078042192.1	lipid A deacylase LpxR family protein	-
B0D95_RS01280 (B0D95_01280)	-	277234..277818 (-)	585	WP_078042193.1	TetR/AcrR family transcriptional regulator	-
B0D95_RS01285 (B0D95_01285)	-	277986..279005 (-)	1020	WP_078045576.1	nucleoid-associated protein	-
B0D95_RS01290 (B0D95_01290)	-	279108..279467 (+)	360	WP_078042194.1	RidA family protein	-
B0D95_RS01295 (B0D95_01295)	pilU	279549..280724 (+)	1176	WP_078042195.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
B0D95_RS01300 (B0D95_01300)	-	280721..283195 (+)	2475	WP_078042196.1	bifunctional acetate--CoA ligase family protein/GNAT family N-acetyltransferase	-
B0D95_RS01305 (B0D95_01305)	-	283233..284183 (-)	951	WP_168172380.1	histone deacetylase family protein	-
B0D95_RS01310 (B0D95_01310)	-	284332..285711 (-)	1380	WP_246841695.1	OmpP1/FadL family transporter	-

Sequence

Protein

• Length: 391 a.a.
• Molecular weight: 43178.49 Da
• Isoelectric Point: 6.5969

>NTDB_id=218412 B0D95_RS01295 WP_078042195.1 279549..280724(+) (pilU) [Cellvibrio sp. PSBB023]
MATPIDQYLNILAKKDGSDLYLSTGAPPCAKFQGVLKPLTNEPFKPGDIERIANAIMDDEQRAIFAHELEMNLAISIHGV
GRFRINIFKQRNEVAIVARNIKTDIPQFDDLGLPEILKEVIMTKRGLVLFVGGTGSGKSTSLAALIDHRNSSSGGHIITI
EDPVEFVHKHKRSVINQREVGVDTRSYKAALKNTLRQAPDVILIGEIRDRETMEHALEFAETGHLAISTLHANNANQALE
RIVNLFPEERRPQLLMGLSQNLRAFVSQRLVPTVDGKRCAAVEVLLGTKTIQELIFKNRFTEIKEIMEKSENLGMQTFDG
AMFKLYMAGRISFDDAIANADSPNNLRLRIKLATDGVAPAKAETAAVKPSATAGSFGELSLQKIDEDDENQ

Nucleotide

• Length: 1176 bp

>NTDB_id=218412 B0D95_RS01295 WP_078042195.1 279549..280724(+) (pilU) [Cellvibrio sp. PSBB023]
ATGGCAACACCCATTGATCAATACTTGAATATTCTTGCGAAAAAAGACGGTTCAGATCTGTACCTCAGCACCGGTGCACC
TCCCTGCGCCAAGTTTCAAGGTGTGTTAAAACCGCTTACCAATGAACCCTTTAAGCCGGGCGATATAGAACGTATTGCCA
ATGCCATCATGGATGATGAACAGCGCGCTATTTTTGCGCACGAATTAGAAATGAATTTGGCGATCTCCATTCATGGTGTC
GGGCGCTTTCGTATTAATATTTTCAAGCAGCGCAATGAAGTGGCAATTGTGGCGCGTAACATTAAAACCGATATTCCGCA
ATTTGATGACCTCGGCCTGCCTGAAATTTTAAAAGAAGTCATTATGACCAAACGCGGCTTGGTGTTGTTTGTCGGCGGCA
CTGGCTCAGGGAAATCAACATCGTTGGCAGCCTTGATAGATCATCGCAATTCCTCCAGTGGCGGTCACATTATTACCATA
GAGGACCCGGTTGAGTTTGTGCATAAACACAAGCGCAGTGTCATTAATCAGCGCGAAGTGGGTGTGGATACGCGCAGTTA
TAAAGCAGCTCTCAAAAATACCCTGCGCCAAGCGCCCGATGTCATTTTGATTGGTGAGATTCGCGATCGTGAAACCATGG
AGCATGCCCTTGAGTTTGCTGAGACAGGGCATTTGGCAATTTCCACTTTGCATGCAAACAATGCCAATCAGGCGCTTGAG
CGGATTGTTAACCTGTTTCCGGAGGAGCGCCGGCCACAATTATTAATGGGGCTCTCACAAAATTTACGTGCGTTTGTGTC
CCAGCGGCTGGTGCCTACGGTCGATGGAAAGCGCTGCGCTGCTGTTGAAGTATTGCTCGGTACCAAAACTATTCAGGAGT
TGATTTTTAAAAATCGCTTTACTGAAATTAAAGAGATTATGGAGAAATCGGAAAATCTCGGGATGCAAACTTTTGATGGC
GCCATGTTTAAATTGTACATGGCCGGTAGAATCAGTTTTGACGATGCTATTGCCAATGCCGATTCACCCAACAACTTGCG
TTTGCGTATCAAACTGGCAACGGATGGTGTCGCACCTGCAAAAGCAGAAACAGCAGCGGTGAAGCCCAGTGCAACAGCGG
GTAGTTTTGGTGAATTGAGCTTGCAAAAAATTGATGAAGACGACGAAAACCAATGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (107-271)

3D structure

• AlphaFold DB: A0A1U9N5I8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	54.085	90.793	0.491
pilU	Acinetobacter baylyi ADP1	52.691	90.281	0.476
pilU	Vibrio cholerae strain A1552	50	89.003	0.445
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	40.217	94.118	0.379