Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   A8E75_RS32930 Genome accession   NZ_CP019674
Coordinates   3133745..3135250 (+) Length   501 a.a.
NCBI ID   WP_006483132.1    Uniprot ID   -
Organism   Burkholderia cenocepacia strain VC12308     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3128745..3140250
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A8E75_RS32905 (A8E75_32945) kdsD 3129976..3130959 (+) 984 WP_006483136.1 arabinose 5-phosphate isomerase KdsD -
  A8E75_RS32910 (A8E75_32950) - 3130959..3131495 (+) 537 WP_006483148.1 KdsC family phosphatase -
  A8E75_RS32915 (A8E75_32955) lptC 3131499..3132113 (+) 615 WP_006483145.1 LPS export ABC transporter periplasmic protein LptC -
  A8E75_RS32920 (A8E75_32960) lptA 3132134..3132799 (+) 666 WP_006483139.1 lipopolysaccharide transport periplasmic protein LptA -
  A8E75_RS32925 (A8E75_32965) lptB 3132796..3133572 (+) 777 WP_006483143.1 LPS export ABC transporter ATP-binding protein -
  A8E75_RS32930 (A8E75_32970) rpoN/rpoN1 3133745..3135250 (+) 1506 WP_006483132.1 RNA polymerase factor sigma-54 Machinery gene
  A8E75_RS32940 (A8E75_32975) hpf 3135409..3135768 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  A8E75_RS32945 (A8E75_32980) - 3135964..3136476 (+) 513 WP_034195643.1 PTS sugar transporter subunit IIA -
  A8E75_RS32950 (A8E75_32985) hprK 3136606..3137574 (+) 969 WP_006483133.1 HPr(Ser) kinase/phosphatase -
  A8E75_RS32955 (A8E75_32990) rapZ 3137649..3138557 (+) 909 WP_006483144.1 RNase adapter RapZ -
  A8E75_RS32960 (A8E75_32995) - 3138584..3139219 (+) 636 WP_006483137.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54735.87 Da        Isoelectric Point: 4.8740

>NTDB_id=217733 A8E75_RS32930 WP_006483132.1 3133745..3135250(+) (rpoN/rpoN1) [Burkholderia cenocepacia strain VC12308]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPAQNTEPAA
TNGSSPSSDRAERDEPAGADEYDGYGSGDGNDGPQWNLDEYGRSSGASDDDDLPPLQVQEAATSLRDHLAAQLRVTQAGP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLLRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGKAEADYVVPDIIVKKAGQGWLAELNPEVVP
RLRINNLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=217733 A8E75_RS32930 WP_006483132.1 3133745..3135250(+) (rpoN/rpoN1) [Burkholderia cenocepacia strain VC12308]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTTGCGCTGACGCCTCAGCTGCAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAACTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGCTGCGCGTCGCGGCGGACGGATCGCTGATCGCGCAGACGCCGCCCGCACAGAACACCGAGCCCGCCGCG
ACCAACGGCAGCAGCCCGTCCAGCGATCGCGCGGAGCGCGACGAGCCGGCCGGCGCCGACGAATACGACGGCTACGGGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGACGAATACGGCCGCTCGAGCGGCGCGTCCGACGACGACGACCTGC
CGCCGCTGCAGGTGCAGGAAGCCGCGACGTCGCTGCGCGACCACCTCGCCGCGCAGTTGCGCGTCACGCAGGCCGGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAATCGCTCGACGACGACGGCTACCTGACCGCCACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAACTGGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTCGGCGCGCGCTCGGCGTCCGAATGCCTGAAGCTGCAGCTGCTGCGTCTCGATCCGTCCCCGACGCGCACGCTC
GCGCTCGAGATCGTGTCGCAGCACCTCGAACTGCTCGCCGCACGCGACTTCACACGGTTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCACTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCATACGGCAAGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGCTGGCTCGCGGAGCTCAATCCGGAAGTCGTGCCG
CGCCTGCGGATCAACAACCTGTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGATCGCTGAAACAGCA
GCTCCAGGAAGCGCGCTGGCTGATCAAGAATATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCTATTGTCG
AGCGTCAGAAGAATTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTCTCGACCGACACCGGTGGCGCCGCCTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCGCGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.182

100

0.689

Multiple sequence alignment