Detailed information    

insolico Bioinformatically predicted

Overview

Name   recJ   Type   Machinery gene
Locus tag   BEI53_RS14845 Genome accession   NZ_CP018935
Coordinates   2739017..2741356 (+) Length   779 a.a.
NCBI ID   WP_000941281.1    Uniprot ID   A0A6H9HZR4
Organism   Bacillus cereus strain JEM-2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 2734017..2746356
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BEI53_RS14830 - 2735049..2735333 (+) 285 WP_000886978.1 post-transcriptional regulator -
  BEI53_RS14835 secDF 2735604..2737868 (+) 2265 WP_001119059.1 protein translocase subunit SecDF -
  BEI53_RS14840 - 2738022..2738915 (+) 894 WP_000409725.1 cation diffusion facilitator family transporter -
  BEI53_RS14845 recJ 2739017..2741356 (+) 2340 WP_000941281.1 single-stranded-DNA-specific exonuclease RecJ Machinery gene
  BEI53_RS14850 - 2741404..2741916 (+) 513 WP_000346214.1 adenine phosphoribosyltransferase -
  BEI53_RS14855 relA 2742127..2744310 (+) 2184 WP_001262809.1 GTP diphosphokinase -
  BEI53_RS14860 - 2744328..2744768 (+) 441 WP_075396063.1 D-tyrosyl-tRNA(Tyr) deacylase -
  BEI53_RS14865 - 2745037..2745228 (+) 192 WP_001104596.1 hypothetical protein -
  BEI53_RS29565 - 2745314..2745490 (+) 177 WP_001242605.1 hypothetical protein -

Sequence

Protein


Download         Length: 779 a.a.        Molecular weight: 88111.71 Da        Isoelectric Point: 4.9330

>NTDB_id=212201 BEI53_RS14845 WP_000941281.1 2739017..2741356(+) (recJ) [Bacillus cereus strain JEM-2]
MLQPKTRWKEKEYSGERVSELASKLQLSPLVVSLFLGRGLDTEDKILDFLNTENQEFHDPFLLEGMDRTVERVNKAIQNG
EQILIFGDYDADGVSSTTVLYLALQELGADVEFYIPNRFTEGYGPNEEAFRWAHSAGFSLIITVDTGIAAVHEAKVAKEL
GIDLIITDHHEPPPELPEALAIIHPKLDGGVYPFHYLAGVGVAFKVAHALLGRVPEHLLEIAVIGTVADLVSLHGENRLL
VKRGLKHMRMTKNIGLKALFKVANVSQSEITEESIGFSIAPRINAVGRLEDATPAVHLLLSEDPEEAKELAEEIDELNKL
RKDIVKQITEEAIAEVETNFPPEDNKVLVLAKEGWNPGVIGIVASKLVERFYRPTIVLCIDPVKETAKGSARSIAGFDLF
ANLSDCRELLPHFGGHPMAAGMTLHMNDVDELRRRLNEQAEVILTEEDFIPITTVDAFCKVEDVTLAAIEDMQKLAPFGV
GNPKPRIAVKDAELESIRAIGSDGSHLKMALRDGQATLDTIGFGFGAYAKEISPVAKVSVIGEASINEWNNFKKPQLMVQ
DIAVEAWQLFDWRSMRNVEANVAELPKEKITMVYFSKEVLHKFSLEDYKEHMMHASEVTELDEQYIVLLDLPKGTDELRD
LFKVGFPSRIYTLFYQENNHLFSTVPTRDHFKWYYSFLSQKSPFSLRQYGEQLCQHKGWSKDTVNFMTQVFFELEFVTIK
DGVIFMADKKQKRDLIESNTYREKMNHLQLEKELVYSTYQQLYTWFETIRNHKEVEQLG

Nucleotide


Download         Length: 2340 bp        

>NTDB_id=212201 BEI53_RS14845 WP_000941281.1 2739017..2741356(+) (recJ) [Bacillus cereus strain JEM-2]
GTGTTACAACCGAAGACGCGTTGGAAAGAAAAAGAATATAGTGGCGAGCGAGTGAGTGAATTAGCAAGCAAATTACAATT
ATCACCGCTTGTCGTTTCGTTATTCCTCGGTAGAGGACTAGATACAGAAGATAAGATTTTAGATTTTTTAAATACAGAAA
ATCAAGAATTTCATGATCCATTTTTATTAGAAGGAATGGATAGAACGGTAGAACGTGTAAATAAGGCGATTCAAAATGGA
GAGCAAATATTAATATTTGGTGACTACGACGCGGACGGAGTAAGTAGTACGACTGTATTATATCTTGCCCTACAAGAATT
AGGTGCAGACGTAGAATTTTATATCCCAAACCGTTTTACAGAAGGTTATGGACCAAACGAAGAAGCATTCCGTTGGGCGC
ATAGCGCAGGATTTTCACTCATTATTACTGTTGATACTGGTATCGCAGCTGTACATGAAGCGAAAGTAGCGAAGGAACTT
GGGATAGATCTTATTATTACGGATCACCATGAGCCGCCACCAGAATTGCCAGAAGCACTTGCGATTATTCATCCGAAATT
AGATGGCGGTGTGTATCCGTTTCACTATTTAGCGGGCGTTGGTGTTGCGTTTAAAGTTGCGCATGCACTACTGGGCCGTG
TACCAGAACATTTATTAGAAATTGCAGTAATTGGTACAGTAGCCGATTTAGTATCACTTCACGGTGAAAATAGATTGCTA
GTGAAGCGCGGTTTAAAACATATGCGCATGACGAAAAATATTGGACTAAAGGCGCTATTTAAAGTAGCTAACGTTTCGCA
AAGTGAAATTACAGAAGAGAGTATTGGCTTCTCAATCGCACCTCGTATTAATGCAGTTGGACGTTTAGAAGATGCTACGC
CGGCTGTACATCTTTTATTATCAGAGGATCCGGAAGAAGCGAAAGAGCTAGCTGAAGAAATTGATGAGTTGAACAAACTG
CGAAAAGATATTGTAAAGCAAATTACAGAAGAAGCTATCGCTGAAGTGGAAACGAATTTCCCGCCTGAAGACAATAAAGT
ATTAGTGCTTGCAAAAGAAGGATGGAATCCAGGTGTAATTGGAATTGTTGCTTCTAAATTAGTTGAACGTTTTTATCGTC
CAACGATTGTATTATGCATTGATCCTGTAAAAGAAACTGCAAAAGGTTCAGCGCGTAGTATTGCAGGATTCGATTTGTTT
GCAAACTTGTCAGATTGTAGAGAGTTATTGCCACACTTCGGGGGACATCCGATGGCAGCGGGAATGACGCTACATATGAA
TGATGTAGATGAATTACGCCGCCGTTTAAATGAGCAAGCGGAGGTAATTTTAACGGAAGAAGATTTTATCCCAATTACAA
CTGTCGATGCTTTTTGTAAAGTGGAGGACGTAACACTTGCAGCAATCGAGGATATGCAAAAGTTGGCACCATTTGGTGTT
GGAAATCCAAAGCCACGTATTGCGGTGAAGGATGCAGAGTTAGAAAGTATTCGTGCCATCGGATCAGACGGTTCTCATTT
AAAAATGGCTCTTCGTGATGGACAGGCAACACTTGATACAATCGGGTTTGGATTTGGAGCGTACGCGAAAGAAATTTCTC
CAGTTGCGAAAGTATCTGTAATCGGGGAAGCGTCTATCAATGAATGGAATAATTTTAAGAAGCCGCAATTAATGGTGCAA
GATATTGCTGTAGAGGCATGGCAATTATTTGATTGGCGTAGTATGCGTAACGTAGAAGCAAATGTAGCAGAACTTCCGAA
AGAAAAAATAACGATGGTGTATTTTTCTAAAGAGGTATTGCATAAATTTTCTTTAGAAGACTATAAAGAGCATATGATGC
ATGCATCAGAAGTAACCGAGTTAGATGAGCAATACATCGTGTTACTTGATTTACCAAAAGGAACAGATGAATTACGAGAT
CTATTTAAAGTTGGGTTCCCGTCGCGAATTTATACGCTATTTTATCAAGAGAATAATCATTTATTTAGTACTGTTCCAAC
GAGGGATCACTTTAAATGGTACTATTCATTCTTGAGCCAAAAATCACCATTTTCTTTAAGACAATATGGAGAACAACTAT
GTCAACATAAAGGTTGGTCAAAAGATACAGTAAATTTCATGACACAGGTGTTTTTTGAGTTAGAATTTGTTACAATAAAA
GATGGCGTCATTTTTATGGCAGACAAAAAACAAAAGCGTGATTTAATTGAATCGAACACATATCGTGAGAAAATGAACCA
CTTACAATTAGAAAAAGAGTTGGTTTATAGCACATATCAGCAACTATATACATGGTTTGAAACGATTCGTAATCATAAAG
AAGTAGAACAGCTAGGGTAA

Domains

Predicted by InterproScan.

DHHA1 domain (DHHA1)

(315-439)

RecJ OB domain (RecJ_OB)

(455-561)

Single-strand DNA-specific exonuclease, C terminal domain (ssDNA-exonuc_C)

(568-766)

DHH family (DHH)

(82-226)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6H9HZR4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recJ Bacillus subtilis subsp. subtilis str. 168

52.972

99.358

0.526

Multiple sequence alignment