Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   S101392_RS13415 Genome accession   NZ_CP021921
Coordinates   2601550..2601960 (-) Length   136 a.a.
NCBI ID   WP_009967785.1    Uniprot ID   P42983
Organism   Bacillus subtilis subsp. subtilis strain SRCM101392     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 2596550..2606960
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  S101392_RS13385 - 2596639..2596779 (+) 141 WP_003226124.1 sporulation histidine kinase inhibitor Sda -
  S101392_RS13390 (S101392_02669) yqeF 2597085..2597816 (-) 732 WP_003229964.1 SGNH/GDSL hydrolase family protein -
  S101392_RS13395 (S101392_02670) cwlH 2598068..2598820 (-) 753 WP_088326549.1 N-acetylmuramoyl-L-alanine amidase CwlH -
  S101392_RS13400 yqeD 2599007..2599632 (+) 626 Protein_2601 TVP38/TMEM64 family protein -
  S101392_RS13405 (S101392_02672) gnd 2599651..2600544 (-) 894 WP_043857850.1 phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) -
  S101392_RS13410 (S101392_02673) yqeB 2600796..2601517 (+) 722 Protein_2603 DUF308 domain-containing protein -
  S101392_RS13415 (S101392_02674) nucA/comI 2601550..2601960 (-) 411 WP_009967785.1 sporulation-specific Dnase NucB Machinery gene
  S101392_RS13420 (S101392_02675) - 2602156..2602506 (+) 351 Protein_2605 sigma-70 family RNA polymerase sigma factor -
  S101392_RS13425 (S101392_02676) - 2602646..2603116 (+) 471 WP_033884465.1 MarR family transcriptional regulator -
  S101392_RS13430 (S101392_02677) - 2603267..2604679 (+) 1413 WP_088326553.1 MDR family MFS transporter -
  S101392_RS13435 (S101392_02678) spoIVCA 2604866..2606326 (-) 1461 WP_250621315.1 site-specific DNA recombinase SpoIVCA -
  S101392_RS21905 - 2606284..2606462 (-) 179 Protein_2609 hypothetical protein -

Sequence

Protein


Download         Length: 136 a.a.        Molecular weight: 14967.97 Da        Isoelectric Point: 5.1853

>NTDB_id=198486 S101392_RS13415 WP_009967785.1 2601550..2601960(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain SRCM101392]
MKKWMAGLFLAAAVLLCLMVPQQIQGASSYDKVLYFPLSRYPETGSHIRDAIAEGHPDICTIDRDGADKRREESLKGIPT
KPGYDRDEWPMAVCEEGGAGADVRYVTPSDNRGAGSWVGNQMSSYPDGTRVLFIVQ

Nucleotide


Download         Length: 411 bp        

>NTDB_id=198486 S101392_RS13415 WP_009967785.1 2601550..2601960(-) (nucA/comI) [Bacillus subtilis subsp. subtilis strain SRCM101392]
ATGAAAAAATGGATGGCAGGCCTGTTTCTTGCTGCAGCAGTTCTTCTTTGTTTAATGGTTCCGCAACAGATCCAAGGCGC
ATCTTCGTATGACAAAGTGTTATATTTTCCGCTGTCTCGTTATCCGGAAACCGGCAGTCATATTAGGGATGCGATTGCAG
AGGGACATCCAGATATTTGTACCATTGACAGAGATGGAGCAGACAAAAGGCGGGAGGAATCTTTAAAGGGAATCCCGACC
AAGCCGGGCTATGACCGGGATGAGTGGCCGATGGCGGTCTGCGAGGAAGGCGGTGCAGGGGCTGATGTCCGATATGTGAC
GCCTTCTGATAATCGCGGCGCCGGCTCGTGGGTAGGGAATCAAATGAGCAGCTACCCTGACGGCACCAGAGTGCTGTTTA
TTGTGCAGTAA

Domains

Predicted by InterProScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(40-130)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P42983

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

62.609

84.559

0.529