Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   A7U44_RS13425 Genome accession   NZ_CP016856
Coordinates   2580755..2581252 (+) Length   165 a.a.
NCBI ID   WP_020978197.1    Uniprot ID   -
Organism   Staphylococcus aureus strain 2148.N     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2575755..2586252
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A7U44_RS13385 (A7U44_1266) - 2575799..2576785 (+) 987 WP_000161314.1 ROK family glucokinase -
  A7U44_RS13390 (A7U44_1267) - 2576785..2577114 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  A7U44_RS13395 (A7U44_1268) - 2577111..2577734 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  A7U44_RS13400 (A7U44_1269) comGA 2577786..2578760 (+) 975 WP_000697220.1 competence type IV pilus ATPase ComGA Machinery gene
  A7U44_RS13405 (A7U44_1270) comGB 2578732..2579802 (+) 1071 WP_000776401.1 competence type IV pilus assembly protein ComGB Machinery gene
  A7U44_RS13410 (A7U44_1271) comGC 2579816..2580127 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  A7U44_RS13415 (A7U44_1272) comGD 2580105..2580551 (+) 447 WP_001788899.1 competence type IV pilus minor pilin ComGD Machinery gene
  A7U44_RS13420 (A7U44_1273) comGE 2580538..2580837 (+) 300 WP_000844410.1 hypothetical protein Machinery gene
  A7U44_RS13425 (A7U44_1274) comGF 2580755..2581252 (+) 498 WP_020978197.1 competence type IV pilus minor pilin ComGF Machinery gene
  A7U44_RS13430 (A7U44_04920) - 2581349..2581495 (+) 147 WP_001789879.1 hypothetical protein -
  A7U44_RS13435 (A7U44_1275) - 2581485..2582009 (+) 525 WP_001015121.1 shikimate kinase -
  A7U44_RS13440 (A7U44_1276) gcvT 2582168..2583259 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  A7U44_RS13445 (A7U44_1277) gcvPA 2583279..2584625 (+) 1347 WP_000019681.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  A7U44_RS13450 (A7U44_1278) gcvPB 2584618..2586090 (+) 1473 WP_000202188.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19158.95 Da        Isoelectric Point: 10.1964

>NTDB_id=192898 A7U44_RS13425 WP_020978197.1 2580755..2581252(+) (comGF) [Staphylococcus aureus strain 2148.N]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDKNDIEIRQQRIILQKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=192898 A7U44_RS13425 WP_020978197.1 2580755..2581252(+) (comGF) [Staphylococcus aureus strain 2148.N]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACAGAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus N315

98.788

100

0.988
  comGF Staphylococcus aureus MW2

96.364

100

0.964

Multiple sequence alignment