Detailed information NTG0189835

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: B9L64_RS14895
• Genome accession: NZ_CP020915
• Coordinates: 2878740..2879861 (+)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis strain 50-1
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 2873740..2884861

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
B9L64_RS14875	ycdA	2873745..2874809 (-)	1065	WP_003234750.1	DUF4352 domain-containing protein	-
B9L64_RS21090	-	2874832..2874963 (-)	132	WP_003246334.1	hypothetical protein	-
B9L64_RS14880	ycdB	2875137..2876558 (+)	1422	WP_046380765.1	YcdB/YcdC domain-containing protein	-
B9L64_RS14885	ycdC	2876745..2878106 (+)	1362	WP_046381483.1	YcdB/YcdC domain-containing protein	-
B9L64_RS14890	cwlK	2878114..2878617 (-)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
B9L64_RS14895	rapF	2878740..2879861 (+)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
B9L64_RS14900	ycdF	2879968..2880744 (+)	777	WP_019712418.1	SDR family oxidoreductase	-
B9L64_RS14905	ycdG	2880769..2882454 (+)	1686	WP_003234736.1	alpha-glucosidase	-
B9L64_RS14910	znuA	2882642..2883601 (+)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
B9L64_RS14915	znuC	2883657..2884352 (+)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=189835 B9L64_RS14895 WP_003246478.1 2878740..2879861(+) (rapF) [Bacillus subtilis strain 50-1]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=189835 B9L64_RS14895 WP_003246478.1 2878740..2879861(+) (rapF) [Bacillus subtilis strain 50-1]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGATACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405