Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   A6L32_RS03540 Genome accession   NZ_CP016627
Coordinates   678615..679658 (+) Length   347 a.a.
NCBI ID   WP_002226887.1    Uniprot ID   -
Organism   Neisseria meningitidis strain M22718     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 646824..692938 678615..679658 within 0


Gene organization within MGE regions


Location: 646824..692938
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A6L32_RS03385 (A6L32_03385) - 646824..647993 (-) 1170 WP_019273883.1 DUF6716 putative glycosyltransferase -
  A6L32_RS03390 (A6L32_03390) - 648043..648720 (-) 678 WP_002215827.1 IS1595-like element ISNme3 family transposase -
  A6L32_RS03395 (A6L32_03395) - 648991..652104 (-) 3114 WP_002248201.1 glycosyltransferase -
  A6L32_RS03400 (A6L32_03400) siaC 652256..653305 (-) 1050 WP_002228845.1 polysialic acid capsule biosynthesis protein SiaC -
  A6L32_RS03405 (A6L32_03405) neuA 653306..653992 (-) 687 WP_002215295.1 N-acylneuraminate cytidylyltransferase -
  A6L32_RS03410 (A6L32_03410) neuC 653996..655129 (-) 1134 WP_033908889.1 UDP-N-acetylglucosamine 2-epimerase -
  A6L32_RS03415 (A6L32_03415) ctrA 655264..656439 (+) 1176 WP_002215290.1 capsule polysaccharide export outer membrane protein CtrA -
  A6L32_RS03420 (A6L32_03420) ctrB 656454..657617 (+) 1164 WP_002215287.1 capsule polysaccharide export protein CtrB -
  A6L32_RS03425 (A6L32_03425) - 657617..658414 (+) 798 WP_002215286.1 ABC transporter permease -
  A6L32_RS03430 (A6L32_03430) - 658411..659061 (+) 651 WP_002220117.1 ABC transporter ATP-binding protein -
  A6L32_RS03435 (A6L32_03435) - 659126..661399 (+) 2274 WP_002248203.1 Tex family protein -
  A6L32_RS03440 (A6L32_03440) - 661430..662428 (+) 999 WP_002220118.1 DNA cytosine methyltransferase -
  A6L32_RS03445 (A6L32_03445) - 662421..662765 (+) 345 WP_002228843.1 DNA adenine methylase -
  A6L32_RS03450 (A6L32_03450) galE 662844..663464 (+) 621 Protein_657 UDP-glucose 4-epimerase GalE -
  A6L32_RS03455 (A6L32_03455) rfbB 663465..664547 (+) 1083 WP_002224375.1 dTDP-glucose 4,6-dehydratase -
  A6L32_RS03460 (A6L32_03460) rfbA 664610..665476 (+) 867 WP_002224369.1 glucose-1-phosphate thymidylyltransferase RfbA -
  A6L32_RS03465 (A6L32_03465) rfbC 665530..666087 (+) 558 WP_002224368.1 dTDP-4-dehydrorhamnose 3,5-epimerase -
  A6L32_RS03470 (A6L32_03470) - 666131..668149 (-) 2019 WP_002224367.1 OPT family oligopeptide transporter -
  A6L32_RS03475 (A6L32_03475) dnaJ 668345..669466 (-) 1122 WP_002215274.1 molecular chaperone DnaJ -
  A6L32_RS03485 (A6L32_03485) comE 669735..670199 (-) 465 WP_011798742.1 ComEA family DNA-binding protein Machinery gene
  A6L32_RS03520 (A6L32_03520) dksA 675999..676415 (-) 417 WP_002215267.1 RNA polymerase-binding protein DksA -
  A6L32_RS03525 (A6L32_03525) proC 676562..677353 (-) 792 WP_002215263.1 pyrroline-5-carboxylate reductase -
  A6L32_RS03530 (A6L32_03530) - 677402..677809 (-) 408 WP_002242150.1 hypothetical protein -
  A6L32_RS03535 (A6L32_03535) - 677809..678495 (-) 687 WP_002242149.1 YggS family pyridoxal phosphate-dependent enzyme -
  A6L32_RS03540 (A6L32_03540) pilT 678615..679658 (+) 1044 WP_002226887.1 type IV pilus twitching motility protein PilT Machinery gene
  A6L32_RS03545 (A6L32_03545) - 679821..681047 (+) 1227 WP_002218483.1 PilT/PilU family type 4a pilus ATPase -
  A6L32_RS03550 (A6L32_03550) yccS 681333..683483 (+) 2151 WP_002248193.1 YccS family putative transporter -
  A6L32_RS03560 (A6L32_03560) - 684703..685911 (+) 1209 WP_002212532.1 replication initiation factor domain-containing protein -
  A6L32_RS03565 (A6L32_03565) - 685932..686243 (+) 312 WP_002212531.1 hypothetical protein -
  A6L32_RS03570 (A6L32_03570) - 686247..686447 (+) 201 WP_002212530.1 hypothetical protein -
  A6L32_RS03575 (A6L32_03575) - 686674..686904 (+) 231 WP_002216898.1 hypothetical protein -
  A6L32_RS03580 (A6L32_03580) - 686932..687234 (+) 303 WP_002226957.1 hypothetical protein -
  A6L32_RS14000 - 687350..687631 (+) 282 WP_002212527.1 hypothetical protein -
  A6L32_RS03585 (A6L32_03585) - 687582..689186 (+) 1605 WP_033909464.1 IgG-binding virulence factor TspB family protein -
  A6L32_RS03590 (A6L32_03590) - 689489..689773 (+) 285 WP_002216892.1 DUF2523 domain-containing protein -
  A6L32_RS03595 (A6L32_03595) - 689774..690961 (+) 1188 WP_002212523.1 zonular occludens toxin family protein -
  A6L32_RS03600 (A6L32_03600) - 691176..692132 (+) 957 WP_002212521.1 IS110-like element ISNgo3 family transposase -
  A6L32_RS03610 (A6L32_03610) - 692654..692938 (-) 285 WP_002216166.1 GIY-YIG nuclease family protein -

Sequence

Protein


Download         Length: 347 a.a.        Molecular weight: 38037.48 Da        Isoelectric Point: 7.0893

>NTDB_id=189768 A6L32_RS03540 WP_002226887.1 678615..679658(+) (pilT) [Neisseria meningitidis strain M22718]
MQITDLLAFGAKNKASDLHLSSGISPMIRVHGDMRRINLPEMSAEEVGNMVTSVMNDHQRKIYQQNLEVDFSFELPNVAR
FRVNAFNTGRGPAAVFRTIPSTVLSLEELKAPSIFQKIAESPRGMVLVTGPTGSGKSTTLAAMINYINETQPAHILTIED
PIEFVHQSKKSLINQRELHQHTLSFANALRSALREDPDVILVGEMRDPETIGLALTAAETGHLVFGTLHTTGAAKTVDRI
VDVFPAGEKEMVRSMLSESLTAVISQNLLKTHDGNGRVASHEILIANPAVRNLIRENKITQINSVLQTGQASGMQTMDQS
LQSLVRQGLIAPEVARRRAQNSESMSF

Nucleotide


Download         Length: 1044 bp        

>NTDB_id=189768 A6L32_RS03540 WP_002226887.1 678615..679658(+) (pilT) [Neisseria meningitidis strain M22718]
ATGCAGATTACCGACTTACTCGCCTTCGGCGCTAAAAACAAAGCATCCGACCTTCACCTGAGTTCGGGCATATCCCCTAT
GATTCGGGTTCACGGCGATATGCGGCGCATCAACCTTCCCGAAATGAGCGCGGAAGAGGTCGGTAATATGGTAACTTCGG
TGATGAACGACCACCAGCGGAAAATCTACCAGCAAAACTTGGAAGTCGACTTCTCGTTCGAACTGCCCAACGTCGCCCGA
TTCCGCGTCAACGCCTTCAACACCGGCCGCGGTCCCGCCGCCGTATTCCGCACCATTCCCAGCACCGTCTTATCGCTGGA
AGAATTGAAAGCCCCGAGCATTTTCCAAAAAATCGCAGAATCGCCGCGCGGCATGGTATTGGTTACCGGCCCTACCGGTT
CGGGCAAATCGACCACGCTTGCCGCGATGATCAACTACATCAACGAAACCCAGCCGGCACACATCCTGACCATCGAAGAC
CCGATCGAATTCGTCCACCAAAGCAAAAAATCCCTGATTAACCAGCGCGAGCTGCACCAGCACACCCTCAGCTTCGCCAA
CGCGCTGCGTTCCGCATTGCGCGAAGACCCCGACGTTATCCTTGTCGGCGAGATGCGCGACCCCGAAACCATCGGCTTGG
CACTGACCGCCGCCGAAACCGGACACTTGGTTTTCGGCACGCTGCACACGACCGGCGCAGCAAAAACCGTCGACCGTATC
GTGGACGTATTCCCGGCGGGAGAAAAAGAAATGGTGCGCTCTATGCTGTCCGAATCGCTGACCGCCGTCATCTCCCAAAA
CCTGCTGAAAACGCACGACGGCAACGGCCGTGTCGCCTCGCACGAAATCCTGATTGCCAACCCCGCCGTCCGCAACCTCA
TCCGCGAAAACAAAATCACGCAGATTAACTCCGTCCTGCAAACCGGGCAGGCGAGCGGTATGCAGACAATGGACCAATCG
CTGCAATCGCTGGTGCGCCAAGGGCTGATCGCACCGGAAGTCGCACGCAGACGCGCGCAAAACAGCGAAAGTATGAGTTT
CTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(12-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Neisseria meningitidis 8013

99.712

100

0.997
  pilT Neisseria gonorrhoeae MS11

99.424

100

0.994
  pilT Acinetobacter baumannii D1279779

68.116

99.424

0.677
  pilT Acinetobacter baumannii strain A118

68.116

99.424

0.677
  pilT Acinetobacter nosocomialis M2

68.116

99.424

0.677
  pilT Acinetobacter baylyi ADP1

67.536

99.424

0.671
  pilT Pseudomonas stutzeri DSM 10701

67.246

99.424

0.669
  pilT Pseudomonas aeruginosa PAK

66.667

99.424

0.663
  pilT Legionella pneumophila strain Lp02

64.809

98.271

0.637
  pilT Legionella pneumophila strain ERS1305867

64.809

98.271

0.637
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

64.545

95.101

0.614
  pilT Vibrio cholerae strain A1552

64.545

95.101

0.614
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

53.687

97.695

0.524
  pilU Pseudomonas stutzeri DSM 10701

40.29

99.424

0.401
  pilU Vibrio cholerae strain A1552

38.329

100

0.383
  pilU Acinetobacter baylyi ADP1

38.439

99.712

0.383

Multiple sequence alignment