Detailed information NTG0183071

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: LLUC06_RS06525
• Genome accession: NZ_CP015902
• Coordinates: 1295448..1296296 (+)
• Length: 282 a.a.
• NCBI ID: WP_058220788.1
• Uniprot ID: Q25AY4
• Organism: Lactococcus lactis subsp. lactis strain UC06
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1290448..1301296

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
LLUC06_RS06500 (LLUC06_1264)	ilvN	1291205..1291681 (+)	477	WP_010905839.1	acetolactate synthase small subunit	-
LLUC06_RS06505 (LLUC06_1265)	ilvC	1291727..1292749 (+)	1023	WP_015426406.1	ketol-acid reductoisomerase	-
LLUC06_RS06510 (LLUC06_1266)	ilvA	1292805..1294055 (+)	1251	WP_017864495.1	threonine ammonia-lyase IlvA	-
LLUC06_RS06515 (LLUC06_1267)	budA	1294171..1294881 (+)	711	WP_058220787.1	acetolactate decarboxylase	-
LLUC06_RS06520 (LLUC06_1268)	-	1294922..1295302 (+)	381	WP_003131142.1	RidA family protein	-
LLUC06_RS06525 (LLUC06_1269)	dprA	1295448..1296296 (+)	849	WP_058220788.1	DNA-processing protein DprA	Machinery gene
LLUC06_RS06530 (LLUC06_1270)	topA	1296558..1298690 (+)	2133	WP_023189256.1	type I DNA topoisomerase	-
LLUC06_RS06535 (LLUC06_1271)	trmFO	1298762..1300105 (+)	1344	WP_025017071.1	methylenetetrahydrofolate--tRNA-(uracil(54)- C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO	-
LLUC06_RS06540 (LLUC06_1272)	xerS	1300150..1301220 (-)	1071	WP_003131037.1	tyrosine recombinase XerS	-

Sequence

Protein

• Length: 282 a.a.
• Molecular weight: 32141.18 Da
• Isoelectric Point: 9.5932

>NTDB_id=183071 LLUC06_RS06525 WP_058220788.1 1295448..1296296(+) (dprA) [Lactococcus lactis subsp. lactis strain UC06]
MITNFDLYRWKKAGMTNLGVNKLLKFFHRYDKKISLRQMGQVAQLKSIPNFIEQYKNQDVSKLREEYKIYPSFSLLDEIY
PERLKEIYNPPVLLFYQGDIRLSKTPKLAFVGSRQASSQGIKAVQKIVTELNQNFTIVSGLAKGIDTASHLSAIKNKIPT
IAVIGTGLDIFYPLENRKIQEYLAKNQLILSEYSVGEKPLRYHFPERNRIIAGLSHGVVVIEAKLRSGSLITCERALEEG
RDIFAVPGNIAYGFSDGCNHLIQQGAKLIYQAQDILEEYLYD

Nucleotide

• Length: 849 bp

>NTDB_id=183071 LLUC06_RS06525 WP_058220788.1 1295448..1296296(+) (dprA) [Lactococcus lactis subsp. lactis strain UC06]
ATGATAACAAATTTTGATTTATATCGTTGGAAAAAAGCTGGCATGACCAATTTAGGAGTAAATAAACTTTTAAAATTTTT
TCATAGATATGATAAAAAAATAAGTTTAAGACAAATGGGACAAGTAGCACAACTTAAATCAATTCCCAACTTTATTGAAC
AATATAAAAATCAAGACGTAAGTAAACTGAGAGAAGAGTATAAAATATACCCATCATTTTCTCTTCTTGATGAAATTTAT
CCCGAAAGACTCAAGGAAATTTACAATCCACCGGTTCTATTATTTTATCAAGGAGATATAAGACTCTCAAAAACTCCTAA
ATTAGCTTTTGTTGGTAGTCGACAAGCAAGCTCACAAGGAATTAAAGCCGTTCAAAAAATTGTGACAGAGTTGAACCAAA
ATTTTACGATTGTCAGCGGACTCGCTAAAGGAATTGATACAGCAAGTCATCTTTCTGCCATTAAAAATAAAATCCCTACA
ATTGCAGTTATCGGTACAGGCTTAGATATCTTTTATCCTTTAGAAAATCGAAAAATTCAAGAGTATCTTGCTAAAAACCA
ACTCATACTATCAGAATATTCAGTCGGAGAAAAGCCACTTCGTTATCATTTTCCTGAACGTAATCGTATCATTGCGGGAC
TTTCACATGGTGTAGTAGTGATAGAAGCAAAGTTACGAAGTGGTAGTTTAATTACTTGTGAGCGCGCTCTGGAAGAAGGA
AGAGATATTTTCGCTGTACCAGGAAACATAGCATATGGATTTTCAGATGGCTGTAATCATCTAATACAACAAGGAGCAAA
ATTAATCTATCAAGCCCAAGATATCTTAGAAGAATATTTGTATGATTAA

Domains

Predicted by InterproScan.

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (2-63)

• DNA recombination-mediator protein A (DNA_processg_A) (76-270)

3D structure

• AlphaFold DB: Q25AY4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Lactococcus lactis subsp. cremoris KW2	86.17	100	0.862
dprA	Streptococcus mutans UA159	59.353	98.582	0.585
dprA/cilB/dalA	Streptococcus mitis SK321	53.929	99.291	0.535
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	53.214	99.291	0.528
dprA/cilB/dalA	Streptococcus pneumoniae D39	53.214	99.291	0.528
dprA/cilB/dalA	Streptococcus pneumoniae R6	53.214	99.291	0.528
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	53.214	99.291	0.528
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	53.214	99.291	0.528
dprA	Legionella pneumophila strain ERS1305867	38.768	97.872	0.379
dprA	Haemophilus influenzae Rd KW20	38.519	95.745	0.369