Detailed information    

insolico Bioinformatically predicted

Overview

Name   crp   Type   Regulator
Locus tag   BWI85_RS10680 Genome accession   NZ_CP019256
Coordinates   2071309..2071941 (-) Length   210 a.a.
NCBI ID   WP_000242755.1    Uniprot ID   A0A1C4FZU4
Organism   Escherichia coli strain 13TMH22     
Function   promote expression of competence genes (predicted from homology)   
Competence regulation

Genomic Context

Location: 2066309..2076941
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWI85_RS10655 (BWI85_10825) yhfG 2066441..2066608 (+) 168 WP_000736860.1 YhfG family protein -
  BWI85_RS10660 (BWI85_10830) fic 2066598..2067200 (+) 603 WP_001280624.1 putative adenosine monophosphate-protein transferase Fic -
  BWI85_RS10665 (BWI85_10835) pabA 2067232..2067795 (+) 564 WP_000601847.1 aminodeoxychorismate synthase component 2 -
  BWI85_RS10670 (BWI85_10840) argD 2067881..2069101 (+) 1221 WP_000963797.1 bifunctional acetylornithine/succinyldiaminopimelate transaminase -
  BWI85_RS10675 (BWI85_10845) yhfK 2069168..2071258 (-) 2091 WP_001297515.1 YccS/YhfK family putative transporter -
  BWI85_RS10680 (BWI85_10850) crp 2071309..2071941 (-) 633 WP_000242755.1 cAMP-activated global transcriptional regulator CRP Regulator
  BWI85_RS10690 (BWI85_10860) yhfA 2072243..2072647 (+) 405 WP_001148908.1 OsmC family protein -
  BWI85_RS10695 (BWI85_10865) prkB 2072702..2073571 (-) 870 WP_001274680.1 phosphoribulokinase -
  BWI85_RS10700 (BWI85_10870) yheU 2073625..2073843 (-) 219 WP_000907085.1 YheU family protein -
  BWI85_RS10705 (BWI85_10875) yheT 2073837..2074859 (-) 1023 WP_000057405.1 hydrolase -
  BWI85_RS10710 (BWI85_10880) yheS 2074859..2076772 (-) 1914 WP_000634793.1 ABC transporter ATP-binding protein -

Sequence

Protein


Download         Length: 210 a.a.        Molecular weight: 23640.43 Da        Isoelectric Point: 8.2710

>NTDB_id=179271 BWI85_RS10680 WP_000242755.1 2071309..2071941(-) (crp) [Escherichia coli strain 13TMH22]
MVLGKPQTDPTLEWFLSHCHIHKYPSKSTLIHQGEKAETLYYIVKGSVAVLIKDEEGKEMILSYLNQGDFIGELGLFEEG
QERSAWVRAKTACEVAEISYKKFRQLIQVNPDILMRLSAQMARRLQVTSEKVGNLAFLDVTGRIAQTLLNLAKQPDAMTH
PDGMQIKITRQEIGQIVGCSRETVGRILKMLEDQNLISAHGKTIVVYGTR

Nucleotide


Download         Length: 633 bp        

>NTDB_id=179271 BWI85_RS10680 WP_000242755.1 2071309..2071941(-) (crp) [Escherichia coli strain 13TMH22]
ATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAA
GAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAG
ACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGC
CAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTACAAAAAATTTCGCCAATTGAT
TCAGGTAAACCCGGACATTCTGATGCGTCTGTCTGCACAGATGGCGCGTCGTCTGCAAGTCACTTCAGAGAAAGTGGGCA
ACCTGGCGTTCCTCGACGTGACGGGCCGCATTGCACAGACTCTGCTGAACCTGGCAAAACAACCAGATGCTATGACTCAC
CCGGACGGTATGCAAATCAAAATTACCCGTCAGGAAATCGGTCAGATTGTCGGCTGTTCTCGTGAAACCGTGGGACGCAT
TCTGAAGATGCTGGAAGATCAGAACCTGATCTCCGCACACGGTAAAACCATCGTCGTTTACGGCACTCGTTAA

Domains

Predicted by InterProScan.

Cyclic nucleotide-binding domain (cNMP_binding)

(23-109)

Crp-like helix-turn-helix domain (HTH_Crp_2)

(142-206)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1C4FZU4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  crp Vibrio cholerae strain A1552

95.714

100

0.957
  crp Haemophilus influenzae Rd KW20

77.941

97.143

0.757
  crp Acinetobacter baumannii D1279779

47.343

98.571

0.467