Detailed information NTG0176744

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: BUN12_RS17635
• Genome accession: NZ_CP018902
• Coordinates: 3372018..3373139 (+)
• Length: 373 a.a.
• NCBI ID: WP_013350919.1
• Uniprot ID: A0A9P1NGF1
• Organism: Bacillus amyloliquefaciens strain HK1
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3367018..3378139

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BUN12_RS17615 (BUN12_3421)	-	3367348..3368343 (-)	996	WP_013350915.1	DUF5105 domain-containing protein	-
BUN12_RS17620 (BUN12_3422)	-	3368461..3369849 (+)	1389	WP_041481592.1	YcdB/YcdC domain-containing protein	-
BUN12_RS17625 (BUN12_3423)	-	3370050..3371408 (+)	1359	WP_013350917.1	YcdB/YcdC domain-containing protein	-
BUN12_RS17630 (BUN12_3424)	-	3371405..3371908 (-)	504	WP_013350918.1	M15 family metallopeptidase	-
BUN12_RS17635 (BUN12_3425)	rapF	3372018..3373139 (+)	1122	WP_013350919.1	tetratricopeptide repeat protein	Regulator
BUN12_RS17640 (BUN12_3426)	-	3373253..3374032 (+)	780	WP_013350920.1	glucose 1-dehydrogenase	-
BUN12_RS17645 (BUN12_3427)	-	3374116..3375795 (+)	1680	WP_013350921.1	glycoside hydrolase family 13 protein	-
BUN12_RS17650 (BUN12_3428)	-	3375780..3376274 (-)	495	WP_013350922.1	DUF4188 domain-containing protein	-
BUN12_RS17655 (BUN12_3429)	-	3376275..3376820 (-)	546	WP_013350923.1	PadR family transcriptional regulator	-
BUN12_RS17660 (BUN12_3430)	-	3377029..3377988 (+)	960	WP_013350924.1	metal ABC transporter substrate-binding protein	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44347.46 Da
• Isoelectric Point: 5.0035

>NTDB_id=176744 BUN12_RS17635 WP_013350919.1 3372018..3373139(+) (rapF) [Bacillus amyloliquefaciens strain HK1]
MKSKIPSEEVAVKMNEWYKLIRAFEADKAEALKREIQYELEDMEENQDLLLYFSLMEFRHRIMLDKLKPMKDGDCQPPFS
EMLTEIEMNQQKLTGLLEYYFYYFRGMYEFKQKNFISAISHYKRAEEKLEYVEDDIEKAEFLYKISEVYYHIKQTFFSMN
YAGQALDIYKKYELYGMRRVQCKFIIAGNLMDAQQHEKALEYLECALEHAERLDDHYMIAAAYYNIGHCMYSLGDFCKAA
EDFERAAAGFEAHSFQQVIQAVFSLTHIYFKTERYSDAAASYARGMKSAAEWEDEIYMTKFRLIKELYQGDGGQDSLKEY
FDILESKELLADTEELLQDAAHFFHKRENYKAASYFFSRLVGVQKRLQMNNYA

Nucleotide

• Length: 1122 bp

>NTDB_id=176744 BUN12_RS17635 WP_013350919.1 3372018..3373139(+) (rapF) [Bacillus amyloliquefaciens strain HK1]
ATGAAATCCAAGATTCCATCTGAAGAAGTAGCCGTAAAGATGAATGAATGGTATAAGCTGATCCGCGCGTTTGAAGCCGA
TAAAGCTGAAGCGCTGAAGCGGGAGATTCAATATGAACTCGAAGATATGGAAGAAAATCAGGATTTATTATTGTATTTTT
CATTGATGGAATTTAGGCATCGCATCATGCTGGATAAATTAAAACCCATGAAAGACGGCGACTGCCAGCCTCCGTTCTCT
GAAATGCTCACTGAGATTGAGATGAACCAGCAAAAGCTGACCGGCTTACTGGAATATTATTTTTACTATTTCAGGGGAAT
GTATGAATTTAAGCAGAAAAACTTCATCTCAGCGATTTCTCATTATAAACGGGCTGAGGAAAAACTAGAATATGTGGAAG
ATGACATAGAGAAAGCCGAGTTTCTGTATAAAATTTCTGAAGTGTACTATCATATCAAGCAGACTTTCTTTTCGATGAAT
TACGCGGGACAGGCGCTTGATATTTACAAGAAATATGAATTATACGGAATGAGAAGGGTGCAGTGCAAATTTATCATTGC
CGGTAACCTGATGGACGCCCAGCAGCATGAAAAAGCGCTGGAATACCTTGAATGCGCCTTGGAGCACGCGGAGCGGCTTG
ATGATCACTATATGATTGCTGCGGCCTATTATAATATCGGGCACTGTATGTACAGTTTGGGTGACTTTTGCAAGGCGGCG
GAAGACTTTGAACGAGCGGCTGCCGGTTTTGAAGCGCACAGCTTCCAGCAGGTCATCCAGGCTGTTTTTTCACTCACGCA
TATCTACTTTAAAACGGAAAGATACAGTGACGCCGCCGCCAGCTATGCGCGGGGCATGAAAAGCGCGGCGGAGTGGGAAG
ACGAGATCTATATGACAAAATTCCGTCTGATTAAGGAGCTTTATCAGGGTGACGGCGGACAGGATTCGCTCAAAGAGTAT
TTTGACATTCTGGAATCAAAGGAGCTGTTGGCCGACACTGAGGAACTGCTTCAGGACGCGGCTCATTTCTTTCATAAACG
GGAGAACTACAAAGCGGCATCTTATTTTTTCAGCAGGCTTGTCGGCGTGCAAAAAAGGCTGCAAATGAACAATTACGCAT
AA

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (187-251)

3D structure

• AlphaFold DB: A0A9P1NGF1

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.588	99.464	0.424
rapC	Bacillus subtilis subsp. subtilis str. 168	40.323	99.732	0.402