Detailed information    

insolico Bioinformatically predicted

Overview

Name   hexA   Type   Machinery gene
Locus tag   BSF20_RS17695 Genome accession   NZ_CP018152
Coordinates   3531317..3533902 (-) Length   861 a.a.
NCBI ID   WP_060657369.1    Uniprot ID   -
Organism   Bacillus amyloliquefaciens strain LM2303     
Function   DNA mismatch repair (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3526317..3538902
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BSF20_RS17675 (BSF20_17320) - 3527067..3527744 (-) 678 WP_003154103.1 MBL fold metallo-hydrolase -
  BSF20_RS17685 (BSF20_17325) - 3528814..3529023 (+) 210 WP_003154104.1 hypothetical protein -
  BSF20_RS17690 (BSF20_17330) hexB 3529424..3531301 (-) 1878 WP_003154106.1 DNA mismatch repair endonuclease MutL Machinery gene
  BSF20_RS17695 (BSF20_17335) hexA 3531317..3533902 (-) 2586 WP_060657369.1 DNA mismatch repair protein MutS Machinery gene
  BSF20_RS17700 (BSF20_17340) cotE 3534021..3534566 (-) 546 WP_003154111.1 outer spore coat protein CotE -
  BSF20_RS17705 (BSF20_17345) ymcA 3534820..3535251 (-) 432 WP_003154113.1 RicAFT regulatory complex protein RicA family protein Regulator
  BSF20_RS17710 (BSF20_17350) miaB 3535254..3536783 (-) 1530 WP_003154116.1 tRNA (N6-isopentenyl adenosine(37)-C2)-methylthiotransferase MiaB -
  BSF20_RS17715 (BSF20_17355) - 3536926..3538104 (-) 1179 WP_088037143.1 glycine C-acetyltransferase -

Sequence

Protein


Download         Length: 861 a.a.        Molecular weight: 97729.02 Da        Isoelectric Point: 5.4210

>NTDB_id=173294 BSF20_RS17695 WP_060657369.1 3531317..3533902(-) (hexA) [Bacillus amyloliquefaciens strain LM2303]
MASYTPMIQQYLKIKAEHQDAFLFFRLGDFYEMFFEDAKKASQELEITLTSRDGGSADKIPMCGVPYHSAAAYIEQLIKK
GYKVAICEQTEDPKAAKGVVKREVVQLITPGTVMDGKGIHESENNFIASVSEFRNGYGLALSDLTTGENLAVFIERIEDV
MSEIYSVGAKEIVVSSKFNEHTAARLKERCGATISIEDGEITERIEIAKHLPGEELTETFMRLYTYLQKTQKRSLDHLQP
VQVYELEEAMKIDLYSKRNLELTETIRSKSKKGSLLWLLDETKTAMGGRLLKQWIDRPLIRASQIEERQEMVETLINHLF
EREDLRERLKEVYDLERLAGRVAFGNVNARDLIQLKESLKQVPSIKELVGSLNHKKAKERAGLIDPCGDLLDLLEEALHE
NPPLSLKEGNLIKDGYHQKLDEYRDASKNGKDWIARLEQQERAYTGIRSLKVGFNKVFGYYIEVTKANLHLLEDGRYERK
QTLTNAERYITPELKEKEALILEAENNICELEYELFAGLREKVKQFIPRLQRLAKQMSELDALQCFATISENRHYTKPVF
SDNEVKVIEGRHPVVEKVMDSQEYVPNNCLMGDSREMLLITGPNMSGKSTYMRQIALLSIMAQIGCFVPAKEAVLPIFDQ
IFTRIGAADDLISGQSTFMVEMLEAKNAIVNATKDSLILFDEIGRGTSTYDGMALAQAIIEYVHDHIGAKTLFSTHYHEL
TVLEDKLPQLKNVHVRAEEYNGTVVFLHQIKEGAADKSYGIHVAQLAELPDDLISRAQEILKQLEQTGDKPELPAAPEKK
PAVKEEPAQLSFFADGEKEQKAPAVSNKEKQVLEAFKSINILDMTPLEAMNEMYKLQKKLK

Nucleotide


Download         Length: 2586 bp        

>NTDB_id=173294 BSF20_RS17695 WP_060657369.1 3531317..3533902(-) (hexA) [Bacillus amyloliquefaciens strain LM2303]
ATGGCTAGTTACACGCCTATGATACAGCAATACTTGAAGATAAAGGCAGAGCACCAAGATGCCTTTTTATTTTTTCGGCT
TGGTGACTTTTATGAAATGTTTTTTGAAGATGCAAAAAAAGCGTCGCAAGAGCTTGAAATTACACTGACAAGCAGAGACG
GAGGTTCGGCTGATAAAATTCCGATGTGCGGCGTGCCGTATCATTCCGCGGCGGCTTATATTGAACAGCTGATTAAAAAG
GGCTATAAAGTGGCGATCTGTGAGCAGACGGAAGATCCGAAGGCGGCTAAAGGCGTTGTAAAAAGAGAAGTCGTCCAGCT
GATCACCCCGGGAACCGTGATGGATGGAAAAGGCATCCATGAGTCGGAAAACAACTTTATCGCTTCCGTTTCCGAATTTC
GTAACGGATACGGTCTCGCTTTATCAGACTTAACGACCGGAGAAAACCTGGCCGTATTTATTGAGCGGATTGAAGATGTC
ATGTCAGAAATTTACTCAGTCGGCGCAAAGGAAATTGTCGTATCAAGCAAGTTTAACGAACATACGGCTGCTCGGCTGAA
AGAACGGTGCGGCGCAACGATTTCCATCGAAGACGGGGAAATAACAGAACGCATTGAAATCGCAAAGCACTTGCCGGGCG
AAGAGCTGACAGAGACGTTTATGCGTTTATATACGTATTTGCAGAAAACGCAAAAACGGAGCCTGGATCATCTGCAGCCG
GTTCAAGTATATGAATTGGAAGAAGCGATGAAAATTGACCTGTATTCAAAACGGAATCTTGAGCTGACGGAAACGATCCG
TTCGAAAAGCAAAAAAGGGTCATTGCTTTGGCTTTTAGACGAGACGAAAACAGCAATGGGCGGCCGGCTTCTCAAACAAT
GGATTGACCGCCCGCTGATCAGGGCATCCCAAATCGAAGAACGCCAGGAAATGGTCGAGACGCTGATCAATCATCTGTTT
GAGCGCGAAGATCTGCGCGAGCGCCTGAAGGAAGTATACGATTTAGAACGTCTGGCGGGCCGGGTCGCATTCGGAAACGT
CAACGCAAGAGATTTAATCCAGCTGAAAGAATCTTTAAAACAAGTGCCGAGCATTAAAGAGCTTGTAGGATCTCTTAACC
ATAAAAAAGCAAAAGAACGCGCCGGCTTGATTGATCCGTGCGGAGATTTGCTTGACCTGTTAGAAGAAGCCCTTCATGAA
AACCCGCCGTTATCGTTAAAAGAAGGCAACTTAATTAAAGACGGCTATCATCAGAAGCTCGATGAGTACCGTGACGCCAG
CAAAAACGGTAAAGACTGGATTGCCAGACTGGAGCAGCAGGAACGCGCTTATACGGGCATCCGTTCATTAAAGGTCGGTT
TCAATAAAGTGTTCGGCTATTATATTGAAGTCACAAAAGCAAACCTTCATCTGCTTGAGGACGGGCGGTATGAGCGGAAG
CAGACCCTCACAAACGCCGAACGGTACATTACGCCGGAATTGAAGGAAAAAGAAGCGCTTATTTTAGAAGCGGAAAACAA
CATCTGCGAGCTGGAATATGAGCTTTTTGCCGGTCTGCGTGAAAAGGTCAAACAATTTATTCCGCGTCTGCAGCGTCTTG
CCAAACAAATGAGTGAGCTGGATGCGCTGCAATGCTTTGCTACGATCAGTGAAAACAGGCATTACACAAAGCCCGTCTTT
TCTGATAATGAAGTAAAAGTCATTGAAGGGAGACACCCCGTCGTTGAAAAGGTGATGGACAGTCAAGAATACGTGCCGAA
CAACTGCCTGATGGGCGATTCGCGGGAAATGCTCCTGATTACGGGTCCGAATATGTCAGGTAAAAGCACGTACATGAGAC
AGATCGCACTTCTATCGATCATGGCCCAGATCGGCTGTTTTGTGCCCGCAAAAGAAGCGGTTCTGCCGATTTTTGATCAA
ATCTTCACGAGAATCGGGGCGGCGGATGATTTGATTTCCGGACAAAGCACCTTTATGGTGGAAATGCTGGAAGCGAAAAA
CGCGATTGTGAACGCCACAAAAGACAGCCTGATCCTGTTTGATGAAATCGGCCGCGGGACATCAACGTACGACGGAATGG
CGCTGGCACAGGCGATTATTGAATATGTTCATGATCATATCGGCGCTAAAACACTGTTCAGCACGCATTATCATGAGCTG
ACGGTGCTTGAAGATAAGCTTCCTCAGCTGAAAAACGTTCATGTGCGCGCAGAGGAATATAACGGAACGGTCGTGTTTCT
CCATCAAATCAAAGAAGGAGCCGCAGATAAAAGTTACGGCATTCATGTCGCCCAGCTTGCGGAGCTCCCGGACGATCTGA
TCAGCCGCGCGCAGGAGATTTTGAAACAGCTTGAGCAGACGGGAGATAAACCGGAGCTTCCGGCGGCACCTGAGAAAAAG
CCCGCCGTCAAAGAAGAACCGGCACAGCTGTCCTTTTTTGCTGACGGTGAAAAAGAACAGAAGGCACCGGCTGTTTCTAA
TAAAGAAAAACAAGTGCTTGAAGCTTTTAAGTCAATCAATATTCTCGATATGACGCCGCTTGAGGCGATGAATGAAATGT
ACAAGCTGCAAAAAAAATTAAAATAG

Domains

Predicted by InterProScan.

MutS domain I (MutS_I)

(5-116)

MutS domain V (MutS_V)

(598-785)

MutS family domain IV (MutS_IV)

(415-506)

MutS domain III (MutS_III)

(258-547)

MutS domain II (MutS_II)

(125-239)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  hexA Streptococcus pneumoniae R6

48.191

99.535

0.48
  mutS Pseudomonas stutzeri strain ATCC 17587

38.549

100

0.395