Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   AXG89_RS07435 Genome accession   NZ_CP014306
Coordinates   1567846..1569363 (-) Length   505 a.a.
NCBI ID   WP_061998369.1    Uniprot ID   A0A0X8L4F0
Organism   Burkholderia sp. PAMC 26561     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1562846..1574363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXG89_RS07410 (AXG89_07335) - 1563696..1564328 (-) 633 WP_062168899.1 LON peptidase substrate-binding domain-containing protein -
  AXG89_RS07415 (AXG89_07340) rapZ 1564364..1565260 (-) 897 WP_062168901.1 RNase adapter RapZ -
  AXG89_RS07420 (AXG89_07345) hprK 1565333..1566301 (-) 969 WP_031357600.1 HPr(Ser) kinase/phosphatase -
  AXG89_RS07425 (AXG89_07350) ptsN 1566465..1566920 (-) 456 WP_061998368.1 PTS IIA-like nitrogen regulatory protein PtsN -
  AXG89_RS07430 (AXG89_07355) raiA 1567320..1567673 (-) 354 WP_056357928.1 ribosome-associated translation inhibitor RaiA -
  AXG89_RS07435 (AXG89_07360) rpoN/rpoN1 1567846..1569363 (-) 1518 WP_061998369.1 RNA polymerase factor sigma-54 Machinery gene
  AXG89_RS07440 (AXG89_07365) lptB 1569531..1570298 (-) 768 WP_172811958.1 LPS export ABC transporter ATP-binding protein -
  AXG89_RS07445 (AXG89_07370) lptA 1570310..1570948 (-) 639 WP_236873415.1 lipopolysaccharide transport periplasmic protein LptA -
  AXG89_RS07450 (AXG89_07375) lptC 1571057..1571653 (-) 597 WP_061998372.1 LPS export ABC transporter periplasmic protein LptC -
  AXG89_RS07455 (AXG89_07380) - 1571659..1572222 (-) 564 WP_086386421.1 HAD hydrolase family protein -
  AXG89_RS07460 (AXG89_07385) kdsD 1572222..1573205 (-) 984 WP_062168906.1 arabinose 5-phosphate isomerase KdsD -

Sequence

Protein


Download         Length: 505 a.a.        Molecular weight: 55710.97 Da        Isoelectric Point: 5.0819

>NTDB_id=170233 AXG89_RS07435 WP_061998369.1 1567846..1569363(-) (rpoN/rpoN1) [Burkholderia sp. PAMC 26561]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENEDDWTTSPLRVAADGSLITTGASVSAPEPMM
SNGTSSSSSTTERSENGEPQGVDEYNGMSSSDNGPDSSSWNLEDYGRSNSGSDDDDLPPLQIHESTTTLRDHLMSQLRMT
KANQRDRALITFLIESLDEDGYLTSSLDEVKADLPDELEVDLDELSAALALLQSFDPPGVGGRSASECLKLQLLRLESTP
IRTLALDIVMHHLELLAARDFTRLRKQLKASDDALRDAHLLIRSLEPFPGAAFGKSEADYVVPDILVRKMSSGWTAELNP
EIVPRLRINHLYANILRNNRGDPGSGSLRQQLQEARWLIKNIQQRFETILRVAQAIVERQKNFFAHGEIAMRPLVLREIA
DTLGLHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDSKSPLSDSRIAELLAEQGF
VVARRTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1518 bp        

>NTDB_id=170233 AXG89_RS07435 WP_061998369.1 1567846..1569363(-) (rpoN/rpoN1) [Burkholderia sp. PAMC 26561]
ATGAAAGCCAGCCTCCAACTACGCCTATCGCAGCATCTTGCGCTGACCCCACAACTGCAGCAGTCCATCCGGCTGCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCTGTCGCGCAGAACCCGTTGCTCGAAAACGAGGACGACTGGA
CCACGAGTCCGTTGCGCGTGGCGGCGGACGGCTCGCTGATCACCACAGGCGCTTCGGTGAGCGCGCCCGAGCCCATGATG
AGCAACGGCACGTCGTCGAGCAGCAGCACGACCGAGCGCTCGGAAAACGGCGAACCGCAGGGCGTCGACGAATACAACGG
CATGAGTTCGTCGGACAACGGCCCGGATTCCAGTTCGTGGAACCTGGAGGACTACGGCCGCTCGAACAGCGGTTCGGACG
ACGATGACCTGCCCCCGCTGCAAATCCACGAATCCACGACCACGCTGCGCGATCACCTGATGTCGCAGTTGCGCATGACA
AAGGCCAATCAGCGCGATCGTGCGCTGATCACGTTTCTGATCGAATCGCTCGACGAAGACGGTTACCTCACGTCCTCACT
CGATGAAGTGAAGGCCGACCTGCCCGACGAACTCGAAGTCGATCTCGACGAGTTGAGCGCTGCGCTCGCACTGCTGCAAA
GTTTCGATCCGCCGGGTGTCGGCGGGCGTTCGGCATCGGAATGTCTCAAGCTGCAGCTTCTCAGGCTCGAATCGACGCCT
ATCAGGACGCTGGCGCTGGATATCGTCATGCATCATCTCGAGCTGCTCGCCGCGCGCGATTTCACGCGCCTTCGCAAGCA
GCTCAAAGCCTCGGATGACGCATTGCGGGACGCCCACCTGCTGATCCGTTCGCTCGAACCATTTCCCGGTGCGGCGTTCG
GCAAGAGCGAGGCGGATTACGTGGTTCCCGACATTCTGGTGCGCAAGATGTCGAGCGGATGGACTGCGGAATTGAATCCG
GAAATCGTCCCTCGGCTGCGAATCAATCACCTTTACGCGAATATTCTGCGCAACAATCGCGGCGATCCGGGCAGCGGTTC
GTTGCGTCAGCAACTGCAGGAAGCACGCTGGTTAATTAAGAATATTCAACAAAGATTCGAGACAATTCTTCGCGTGGCGC
AAGCAATTGTGGAGCGTCAGAAGAACTTTTTCGCGCACGGTGAAATTGCAATGCGGCCCTTGGTTTTGAGGGAGATTGCT
GATACGCTGGGTTTACACGAATCCACTGTCTCACGTGTGACAACCGGCAAGTACATGCTGACCCCGTTCGGGACGCTTGA
ATTCAAGTACTTCTTCGGATCGCACGTTTCTACCGACACGGGTGGCGCGGCATCGTCAACGGCGATCCGGGCACTCATCA
AGCAACTGATAGGAGCCGAAGACTCAAAATCTCCTCTTTCAGACAGCCGCATCGCCGAATTGCTGGCGGAACAAGGGTTC
GTGGTGGCACGCCGCACGGTGGCCAAATATCGCGAAGCCCTGAAAATTCCGGCAGTGAACCTGCGAAAGTCTCTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(348-503)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(142-332)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0X8L4F0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

66.471

100

0.671

Multiple sequence alignment