Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   AXW78_RS19030 Genome accession   NZ_CP014282
Coordinates   3848285..3848719 (-) Length   144 a.a.
NCBI ID   WP_000811494.1    Uniprot ID   A0A9Q6M5X1
Organism   Bacillus thuringiensis strain Bt185     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 3843285..3853719
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AXW78_RS19015 (AXW78_19015) - 3844464..3845021 (+) 558 WP_000062058.1 PadR family transcriptional regulator -
  AXW78_RS19020 (AXW78_19020) metE 3845532..3847820 (+) 2289 WP_061884528.1 5-methyltetrahydropteroyltriglutamate-- homocysteine S-methyltransferase -
  AXW78_RS19025 (AXW78_19025) comJ 3847859..3848248 (-) 390 WP_000424030.1 competence protein ComJ -
  AXW78_RS19030 (AXW78_19030) nucA/comI 3848285..3848719 (-) 435 WP_000811494.1 DNA-entry nuclease Machinery gene
  AXW78_RS19035 (AXW78_19035) - 3848956..3849705 (+) 750 WP_000388466.1 DUF3967 domain-containing protein -
  AXW78_RS19040 (AXW78_19040) kinB 3849744..3851018 (-) 1275 WP_000420360.1 sporulation sensor histidine kinase KinB -
  AXW78_RS19045 (AXW78_19045) - 3851329..3851529 (+) 201 WP_000929280.1 hypothetical protein -
  AXW78_RS19050 (AXW78_19050) - 3851652..3852815 (+) 1164 WP_000434579.1 aminotransferase A -

Sequence

Protein


Download         Length: 144 a.a.        Molecular weight: 16054.43 Da        Isoelectric Point: 8.4331

>NTDB_id=170197 AXW78_RS19030 WP_000811494.1 3848285..3848719(-) (nucA/comI) [Bacillus thuringiensis strain Bt185]
MKQLKGIIISIIAILSILVAVYEVLVPEETSVKKTNTYDQVLEFPKERYPETGKHITDAIKEGHSEVCTIDRGGAADRRK
LSLAPYPSKKGYDRDEWPMAMCKEGGKGAHIEYISPADNRGAGSWVGNKLDKYPDGTRVKFEVK

Nucleotide


Download         Length: 435 bp        

>NTDB_id=170197 AXW78_RS19030 WP_000811494.1 3848285..3848719(-) (nucA/comI) [Bacillus thuringiensis strain Bt185]
ATGAAGCAATTAAAAGGTATTATCATTTCAATTATCGCAATTCTGTCTATTTTAGTAGCAGTTTATGAAGTACTTGTGCC
AGAGGAAACAAGTGTAAAGAAAACGAATACGTATGATCAAGTGTTAGAATTTCCGAAAGAACGTTATCCAGAGACAGGAA
AACATATTACGGATGCTATAAAAGAAGGGCATTCAGAAGTTTGTACAATCGATCGTGGTGGTGCTGCAGATAGAAGGAAA
TTGTCGTTAGCACCATATCCATCAAAAAAAGGGTATGATCGTGATGAATGGCCAATGGCGATGTGCAAGGAAGGCGGAAA
AGGAGCACATATTGAATATATAAGTCCGGCGGATAACCGCGGAGCAGGCTCTTGGGTAGGGAATAAGTTAGATAAATACC
CAGATGGTACGCGAGTAAAATTTGAAGTGAAGTAG

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(48-140)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

69.912

78.472

0.549

Multiple sequence alignment