Detailed information NTG0169809

Bioinformatically predicted

Overview

• Name: pilE
• Type: Machinery gene
• Locus tag: AXF35_RS03300
• Genome accession: NZ_CP014255
• Coordinates: 685964..686413 (-)
• Length: 149 a.a.
• NCBI ID: WP_027223681.1
• Uniprot ID: A0AAX2ISP7
• Organism: Legionella pneumophila subsp. pascullei strain F-4185
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 680964..691413

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AXF35_RS03280 (AXF35_13465)	-	681209..681601 (-)	393	WP_027223677.1	membrane protein	-
AXF35_RS03285 (AXF35_13460)	-	681738..682115 (+)	378	WP_027223678.1	hypothetical protein	-
AXF35_RS03290 (AXF35_13455)	-	682315..683622 (+)	1308	WP_027223679.1	tetratricopeptide repeat protein	-
AXF35_RS03295 (AXF35_13450)	comEC	683655..685886 (-)	2232	WP_027223680.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
AXF35_RS03300 (AXF35_13445)	pilE	685964..686413 (-)	450	WP_027223681.1	type IV pilin protein	Machinery gene
AXF35_RS03305 (AXF35_13440)	-	686425..689934 (-)	3510	WP_027223682.1	pilus assembly protein	-
AXF35_RS03310 (AXF35_13435)	-	689947..690459 (-)	513	WP_027223683.1	pilus assembly PilX family protein	-

Sequence

Protein

• Length: 149 a.a.
• Molecular weight: 16374.62 Da
• Isoelectric Point: 8.3282

>NTDB_id=169809 AXF35_RS03300 WP_027223681.1 685964..686413(-) (pilE) [Legionella pneumophila subsp. pascullei strain F-4185]
MLSNRHCMKNNRMRQSAFTLVEVLISMVIMGILVSIAYPSYLQYIQKSRRADAHATLTQDQIILERCYSQNFSYAAACGA
LPAFPQTTQNGYYTINISNLTATTYTLTATPVGTQATDTKCASMSINQANVKTAVDSSANAQPECWNPS

Nucleotide

• Length: 450 bp

>NTDB_id=169809 AXF35_RS03300 WP_027223681.1 685964..686413(-) (pilE) [Legionella pneumophila subsp. pascullei strain F-4185]
ATGCTTAGTAATCGACATTGTATGAAGAATAACCGCATGAGACAATCTGCGTTTACCCTGGTTGAAGTTCTGATCAGCAT
GGTCATTATGGGAATTCTGGTTTCAATTGCCTATCCATCCTATTTACAATATATCCAAAAGTCCCGTCGTGCCGATGCTC
ATGCTACATTGACACAAGATCAAATCATTTTAGAACGCTGTTACTCACAGAATTTTTCCTATGCTGCAGCGTGTGGTGCC
TTACCAGCATTTCCGCAAACAACGCAGAATGGATACTATACTATCAATATTTCAAACCTGACAGCCACAACCTATACCTT
AACCGCAACCCCTGTCGGAACCCAAGCCACTGATACCAAGTGCGCCAGCATGTCAATCAATCAAGCCAATGTAAAAACAG
CGGTAGATTCCTCTGCTAATGCCCAACCAGAATGCTGGAATCCCAGTTAA

Domains

Predicted by InterproScan.

• Type IV minor pilin ComP, DNA uptake sequence receptor (ComP_DUS) (39-124)

• Prokaryotic N-terminal methylation motif (N_methyl) (12-38)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilE	Legionella pneumophila str. Paris	93.96	100	0.94
pilE	Legionella pneumophila strain ERS1305867	93.96	100	0.94