Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   AL539_RS10285 Genome accession   NZ_CP014053
Coordinates   1858036..1859781 (-) Length   581 a.a.
NCBI ID   WP_165439071.1    Uniprot ID   A0AAP6DAA8
Organism   Vibrio alginolyticus strain FDAARGOS_108     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1853036..1864781
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AL539_RS10265 (AL539_18495) - 1853724..1854563 (-) 840 WP_005381301.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -
  AL539_RS10270 (AL539_18500) - 1854633..1856144 (-) 1512 WP_033907307.1 SPOR domain-containing protein -
  AL539_RS10275 (AL539_18505) aroB 1856197..1857297 (-) 1101 WP_104975646.1 3-dehydroquinate synthase -
  AL539_RS10280 (AL539_18510) aroK 1857330..1857848 (-) 519 WP_005381319.1 shikimate kinase AroK -
  AL539_RS10285 (AL539_18515) pilQ 1858036..1859781 (-) 1746 WP_165439071.1 type IV pilus secretin PilQ Machinery gene
  AL539_RS10290 (AL539_18520) pilP 1859807..1860322 (-) 516 WP_005382766.1 pilus assembly protein PilP Machinery gene
  AL539_RS10295 (AL539_18525) pilO 1860312..1860905 (-) 594 WP_033907305.1 type 4a pilus biogenesis protein PilO Machinery gene
  AL539_RS10300 (AL539_18530) pilN 1860898..1861473 (-) 576 WP_104975648.1 PilN domain-containing protein Machinery gene
  AL539_RS10305 (AL539_18535) pilM 1861457..1862473 (-) 1017 WP_005381324.1 type IV pilus biogenesis protein PilM Machinery gene

Sequence

Protein


Download         Length: 581 a.a.        Molecular weight: 63779.79 Da        Isoelectric Point: 4.4385

>NTDB_id=168084 AL539_RS10285 WP_165439071.1 1858036..1859781(-) (pilQ) [Vibrio alginolyticus strain FDAARGOS_108]
MKMRQGLTRTPRILTGLFSLWLFVFLAPLAAAEEVSSNALKSIDFRTNKDKDAVIVVELASPAAIVDIKRVQEGLSIDLL
NTSVKDEQLYLLDVKDFSTVVESVEVFRDTSTTRLVAHINEEYMHDYRLTGRFLEIKVAKIKPNEKAPDKSILEREGKLI
SINFQDIPVRNVLQLIADYNQFNLVVSDSVEGNLTLRLDGVPWQQVLDIILQVKGLDKRVDGNVILVAPTDELDLREKQQ
LEKQKLTEEMGELSSEIIKVNFAKASDIAEMINGEGNISMLSERGSMTIDERTNSLLIRELPENIEVIREIIESLDIPVK
QVQIEARIVTINEGNMDELGIRWGFTSINGSNTVGGSIENNLATIGLYNGEGDDDGGDADGVGIDDFLNVNLAATSPNAT
SLAFQVAKLGSDTLLDLELSALQQESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVTFKKAVLSLKVTPQI
TPDNRLVLDLSVTQDRPGQVVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNSVDKVPLLGDLPLLGALFRR
SYENVGKSELLIFVTPKVVIQ

Nucleotide


Download         Length: 1746 bp        

>NTDB_id=168084 AL539_RS10285 WP_165439071.1 1858036..1859781(-) (pilQ) [Vibrio alginolyticus strain FDAARGOS_108]
ATGAAAATGAGACAAGGATTAACACGTACACCAAGGATACTTACTGGGCTCTTCTCCTTATGGTTGTTTGTATTTTTAGC
CCCTTTAGCGGCAGCTGAAGAAGTTTCGTCCAATGCCCTAAAAAGTATCGACTTTCGAACTAATAAAGATAAAGACGCCG
TGATTGTCGTCGAATTGGCGTCACCGGCCGCGATTGTAGATATCAAGCGTGTACAGGAAGGGTTAAGTATCGATTTGCTT
AACACTTCAGTAAAAGACGAGCAATTGTATCTCCTAGACGTGAAAGACTTTTCGACTGTGGTTGAGAGCGTAGAAGTGTT
TCGCGACACCTCGACGACGCGCTTGGTTGCGCACATCAATGAAGAATACATGCATGATTACCGTTTAACGGGGCGTTTTT
TGGAAATCAAAGTAGCGAAGATCAAACCCAATGAGAAAGCCCCAGATAAAAGCATTTTAGAAAGAGAAGGTAAGCTTATT
TCGATTAACTTCCAAGATATTCCTGTGCGAAACGTCTTGCAGCTTATTGCTGATTACAACCAATTCAATTTGGTTGTCTC
CGACTCTGTAGAAGGCAACCTCACGCTTCGTCTTGATGGTGTCCCTTGGCAGCAAGTGCTGGATATTATTTTGCAAGTTA
AGGGGTTAGATAAGCGTGTTGATGGCAACGTGATTTTAGTTGCTCCGACTGATGAGCTGGACTTGCGTGAGAAGCAGCAG
TTAGAAAAACAAAAACTGACGGAAGAAATGGGAGAGCTTTCATCTGAAATCATCAAAGTTAATTTTGCTAAAGCATCAGA
CATTGCGGAGATGATCAATGGTGAAGGAAATATCAGCATGCTGTCAGAGCGCGGCAGTATGACCATTGATGAGCGAACTA
ACTCGTTATTGATTAGAGAGTTACCTGAAAACATCGAGGTGATTCGAGAAATTATTGAGTCTTTGGATATTCCAGTTAAA
CAGGTTCAAATTGAGGCGCGTATTGTGACCATCAATGAAGGTAACATGGACGAGTTAGGGATTCGTTGGGGTTTTACATC
TATTAATGGCAGTAATACAGTGGGCGGTTCTATTGAGAACAACCTCGCCACGATCGGTCTTTATAATGGAGAAGGGGACG
ACGACGGTGGTGACGCTGATGGAGTGGGGATCGATGACTTCCTTAATGTAAACTTAGCTGCAACCAGCCCGAATGCGACC
AGTCTTGCTTTCCAAGTTGCGAAGTTAGGATCAGACACACTACTTGATTTGGAACTTTCTGCGCTGCAACAAGAGTCTAA
AGCTGAGATTATCTCGAGCCCACGTTTGATCACTACCAATAAGAAACCAGCCTATATCGAACAAGGTACAGAGATTCCTT
ACCTAGAGTCATCATCAAGTGGTGCAACGACGGTGACGTTCAAAAAGGCGGTATTAAGTTTGAAGGTGACCCCGCAAATC
ACACCGGATAACCGCTTAGTGTTGGATTTAAGTGTCACGCAAGATAGACCTGGACAAGTCGTGAAGACAGGCACTGGTGA
AGCTGTTGCGATTGATACTCAACGTATCGGGACGCAAGTGTTAGTGAATAACGGTGAAACGGTCGTTCTTGGTGGTATTT
TTCAGCACAGCATTACTAACTCGGTGGACAAAGTGCCTTTGTTGGGCGATCTGCCGCTATTAGGTGCGCTTTTTCGTCGA
AGCTATGAGAATGTTGGCAAAAGTGAGCTGTTAATTTTCGTCACGCCTAAGGTGGTGATTCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(256-321)

Bacterial type II and III secretion system protein (Secretin)

(421-579)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

92.771

100

0.928
  pilQ Vibrio cholerae strain A1552

74.742

100

0.749
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

74.742

100

0.749
  pilQ Pseudomonas aeruginosa PAK

39.587

100

0.396
  pilQ Legionella pneumophila strain Lp02

40.998

96.558

0.396
  pilQ Legionella pneumophila strain ERS1305867

40.998

96.558

0.396
  comQ Acinetobacter baylyi ADP1

37.241

99.828

0.372
  pilQ Acinetobacter baumannii D1279779

35.859

100

0.367
  pilQ Acinetobacter baumannii strain A118

35.859

100

0.367

Multiple sequence alignment