Detailed information    

insolico Bioinformatically predicted

Overview

Name   pknB   Type   Regulator
Locus tag   A4W86_RS03745 Genome accession   NZ_CP017272
Coordinates   752659..754542 (+) Length   627 a.a.
NCBI ID   WP_251950913.1    Uniprot ID   -
Organism   Latilactobacillus sakei strain TMW 1.1239     
Function   modulate the activity of ComDE; modulate the activity of VicKR (predicted from homology)   
Competence regulation

Genomic Context

Location: 747659..759542
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A4W86_RS03730 (A4W86_03700) fmt 749605..750561 (+) 957 WP_251950912.1 methionyl-tRNA formyltransferase -
  A4W86_RS03735 (A4W86_03705) rsmB 750551..751894 (+) 1344 WP_016264863.1 16S rRNA (cytosine(967)-C(5))-methyltransferase RsmB -
  A4W86_RS03740 (A4W86_03710) - 751916..752662 (+) 747 WP_016264864.1 Stp1/IreP family PP2C-type Ser/Thr phosphatase -
  A4W86_RS03745 (A4W86_03715) pknB 752659..754542 (+) 1884 WP_251950913.1 Stk1 family PASTA domain-containing Ser/Thr kinase Regulator
  A4W86_RS03750 (A4W86_03720) rsgA 754642..755541 (+) 900 WP_251950914.1 ribosome small subunit-dependent GTPase A -
  A4W86_RS03755 (A4W86_03725) rpe 755565..756227 (+) 663 WP_016264866.1 ribulose-phosphate 3-epimerase -
  A4W86_RS03760 (A4W86_03730) - 756214..756864 (+) 651 WP_016264867.1 thiamine diphosphokinase -
  A4W86_RS03765 (A4W86_03735) rpmB 757213..757398 (-) 186 WP_004265581.1 50S ribosomal protein L28 -
  A4W86_RS03770 (A4W86_03740) - 757701..758063 (+) 363 WP_016264868.1 Asp23/Gls24 family envelope stress response protein -

Sequence

Protein


Download         Length: 627 a.a.        Molecular weight: 68242.88 Da        Isoelectric Point: 5.9848

>NTDB_id=167634 A4W86_RS03745 WP_251950913.1 752659..754542(+) (pknB) [Latilactobacillus sakei strain TMW 1.1239]
MMETGYAVAGRYKIIRPIGEGGMANVYLAQDLILDRQVAVKVLRLDLRNDPDTVRRFTREALATTELNHPNIVSIYDVGE
ENSMQYIIMEYIKGTDLKKYIVEHFPIPYQRVIDIMTQILSAVQNAHAHNIIHRDLKPQNILVDEDGNVKISDFGIAIAL
SETAMTQTNTLLGSVHYLSPEQARGSMATKRSDIYSLGIVLYELLTGMVPFEGESAVSIAIKHFQDEVPPVRNYDPRIPQ
ALENVVLKATAKDPDERYSGVDAMMADLATSLSASRAHEPKFVPSKADDLSETKVIPALDPEGITQTNNDEEQVKEAEPT
QKKVKKPAKSKRKWWLLGGGVVLLLLLATVIAFAVQGSEVTVPDLTDMTQQNAESALTDKKLKVGTVQKTTSQKYSKGHV
IRTTPKAGLSVKSRSTVNLIVSSGRQKYKIKDYTNQAYSDVRDALKDKGLKVKRKYQSSSEVSPGLIIDQSIAAGKRVVP
SKTTITLTVSSGKPGFALHNLSNYTKQGIQDYAAQNGLTVVFSEEYSSSISEGMVISQSPASGTMVQEGDSVSVILSKGE
QPTEHHSIVTSSSNSVIESSAPESSQSSESSASESESESESESESESASESISSSASQSSQSSIQSK

Nucleotide


Download         Length: 1884 bp        

>NTDB_id=167634 A4W86_RS03745 WP_251950913.1 752659..754542(+) (pknB) [Latilactobacillus sakei strain TMW 1.1239]
ATGATGGAAACAGGGTACGCTGTAGCGGGGCGTTATAAGATTATTCGCCCCATCGGTGAAGGCGGCATGGCTAATGTTTA
TTTGGCGCAGGATCTAATTTTAGATCGGCAAGTGGCAGTTAAAGTTTTACGATTAGATCTCCGGAATGATCCAGACACAG
TCCGACGGTTTACGCGAGAAGCGTTGGCAACGACTGAATTAAATCATCCGAATATTGTTAGTATTTACGATGTTGGTGAA
GAAAACAGTATGCAATACATCATTATGGAATATATTAAGGGCACCGATCTTAAGAAATATATCGTGGAACACTTTCCAAT
TCCTTATCAACGCGTAATTGATATTATGACGCAGATTTTATCTGCGGTTCAAAACGCACATGCGCATAATATTATTCACC
GGGATTTAAAACCACAAAATATTTTGGTTGATGAAGACGGCAATGTTAAGATTAGTGACTTTGGGATTGCGATTGCGCTC
AGCGAAACAGCGATGACTCAGACCAACACGTTGTTGGGGTCTGTGCATTATCTCTCACCGGAACAGGCAAGAGGCTCGAT
GGCGACGAAACGGTCTGATATTTACTCGTTAGGAATTGTGCTTTATGAGTTATTGACGGGGATGGTGCCTTTTGAAGGCG
AGTCCGCTGTTTCAATTGCGATTAAGCATTTCCAAGATGAAGTCCCACCAGTACGCAACTATGATCCACGTATTCCGCAA
GCCTTGGAAAATGTCGTTTTAAAAGCGACTGCCAAGGATCCGGATGAACGCTATAGTGGTGTTGATGCAATGATGGCTGA
CTTAGCAACTTCTTTATCAGCTAGTCGTGCGCACGAACCTAAATTTGTGCCATCAAAAGCGGATGATTTGAGTGAGACTA
AGGTGATTCCCGCACTAGATCCAGAAGGCATTACGCAAACCAACAACGATGAGGAACAAGTCAAAGAAGCAGAGCCAACT
CAGAAAAAGGTTAAAAAGCCAGCTAAGTCTAAACGTAAGTGGTGGCTACTAGGTGGCGGTGTTGTTTTATTGCTGTTATT
AGCAACGGTGATTGCTTTTGCAGTTCAGGGGAGCGAAGTAACCGTCCCTGATTTAACGGATATGACACAACAAAATGCGG
AATCTGCTTTGACCGATAAAAAACTGAAGGTCGGCACGGTTCAAAAAACAACGAGTCAGAAATACAGTAAGGGCCACGTC
ATTCGAACAACGCCTAAAGCGGGCCTGAGTGTAAAATCAAGAAGTACGGTTAATTTAATTGTCAGTTCAGGACGCCAAAA
GTATAAGATTAAAGACTATACGAACCAAGCATATAGTGATGTTCGGGATGCTTTGAAGGATAAAGGCTTGAAAGTTAAAC
GGAAATACCAAAGTTCATCTGAAGTGAGTCCTGGGTTGATTATCGATCAAAGTATTGCGGCTGGCAAAAGGGTCGTTCCA
AGTAAAACGACGATTACCTTAACAGTCAGCAGCGGCAAACCTGGTTTTGCATTGCATAATCTAAGCAACTATACAAAGCA
AGGGATTCAAGACTATGCTGCACAAAATGGTTTAACGGTCGTATTCTCTGAAGAATATTCATCAAGTATTTCTGAAGGAA
TGGTAATCAGTCAATCGCCTGCAAGTGGTACAATGGTTCAAGAAGGTGATAGCGTAAGCGTTATCCTTTCAAAAGGCGAG
CAACCAACTGAACATCATTCTATTGTGACGTCGAGTTCAAATTCAGTTATCGAGTCGAGTGCGCCTGAATCAAGTCAATC
GAGTGAAAGTAGCGCGAGTGAGTCAGAGAGCGAATCAGAGAGTGAATCGGAAAGCGAATCAGCAAGTGAATCGATCAGTA
GTTCAGCTAGTCAAAGTAGTCAATCGAGCATTCAATCAAAGTAA

Domains

Predicted by InterProScan.

Protein kinase domain (Pkinase)

(12-263)

PASTA domain (PASTA)

(429-490)

PASTA domain (PASTA)

(360-422)

PASTA domain (PASTA)

(500-558)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pknB Streptococcus mutans UA159

41.88

93.301

0.391
  stkP Streptococcus pneumoniae TIGR4

40.304

94.577

0.381
  stkP Streptococcus pneumoniae D39

40.135

94.577

0.38
  stkP Streptococcus pneumoniae R6

40.135

94.577

0.38
  stkP Streptococcus pneumoniae Rx1

39.966

94.577

0.378

Multiple sequence alignment