Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   AWC33_RS00075 Genome accession   NZ_CP013921
Coordinates   17263..18627 (+) Length   454 a.a.
NCBI ID   WP_040166985.1    Uniprot ID   -
Organism   Lacticaseibacillus paracasei strain KL1     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 12263..23627
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AWC33_RS00050 (AWC33_00050) - 12430..13776 (-) 1347 WP_040166982.1 aminopeptidase C -
  AWC33_RS00055 (AWC33_00055) - 14135..15454 (-) 1320 WP_003603256.1 C1 family peptidase -
  AWC33_RS00060 (AWC33_00060) - 15699..16076 (+) 378 WP_003661802.1 DUF805 domain-containing protein -
  AWC33_RS00065 (AWC33_00065) - 16193..16483 (-) 291 WP_003567258.1 GNAT family N-acetyltransferase -
  AWC33_RS00070 (AWC33_00070) - 16704..17246 (+) 543 WP_016376054.1 deoxyuridine 5'-triphosphate nucleotidohydrolase -
  AWC33_RS00075 (AWC33_00075) radA 17263..18627 (+) 1365 WP_040166985.1 DNA repair protein RadA Machinery gene
  AWC33_RS00080 (AWC33_00080) - 18650..19765 (+) 1116 WP_003567252.1 PIN/TRAM domain-containing protein -
  AWC33_RS00085 (AWC33_00085) - 20068..20955 (+) 888 WP_003603253.1 ABC transporter ATP-binding protein -
  AWC33_RS00090 (AWC33_00090) - 20973..21749 (+) 777 WP_025376282.1 ABC transporter permease -
  AWC33_RS00095 (AWC33_00095) - 22067..23338 (+) 1272 WP_016371648.1 MucBP domain-containing protein -

Sequence

Protein


Download         Length: 454 a.a.        Molecular weight: 49084.44 Da        Isoelectric Point: 7.7387

>NTDB_id=166075 AWC33_RS00075 WP_040166985.1 17263..18627(+) (radA) [Lacticaseibacillus paracasei strain KL1]
MAKAKTQYVCQNCGYISATYLGRCPNCGGWNTLVEETVSSSKSVPRQTATGSKVKPTRMNDVTITKGTRVKTGLDELNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSGQLANTGGTVLYVSGEESASQIKMRAGRLGVANSGMYLYPETDMPSIEDVI
NQMQPDYVVIDSVQTMNVPEMNSAVGSVAQIREVTAELMRIAKSKGVTIFIVGHVTKEGAIAGPKILEHMVDTVLYFEGD
MHHTYRILRSVKNRFGSTNEIGIFEMHQDGLQEVANPSEIFLEERLAGATGSAVVVSMEGTRPILVEIQALISPTMYGNA
KRTSSGLDHNRVSLIMAVLEKRANLMLQNQDAYLKATGGVKLDEPAIDLAMAVAIASSYRDKEISPTDCFVGEIGLTGEV
RRVNRIEERVKEAAKLGFKRIFVPRNNLQGWHAPKDIQVIGVTSIAEALHKVFN

Nucleotide


Download         Length: 1365 bp        

>NTDB_id=166075 AWC33_RS00075 WP_040166985.1 17263..18627(+) (radA) [Lacticaseibacillus paracasei strain KL1]
ATGGCGAAAGCAAAAACACAATATGTTTGCCAAAACTGTGGCTATATTTCAGCCACTTATCTAGGCCGGTGCCCAAATTG
CGGCGGCTGGAACACCTTAGTGGAAGAAACCGTCAGTTCAAGCAAGTCTGTGCCGCGACAAACAGCAACCGGCAGTAAGG
TAAAGCCGACGCGGATGAACGATGTGACGATCACCAAAGGAACGCGCGTCAAGACTGGCTTAGATGAACTGAACCGGGTG
CTTGGCGGCGGAGTGGTGCCAGGGTCACTGGTGCTGATCGGCGGGGATCCGGGGATTGGTAAATCAACCTTGCTATTACA
AGTGTCAGGGCAGTTGGCTAATACCGGCGGAACCGTTTTGTATGTCTCTGGCGAAGAAAGTGCCAGTCAGATCAAAATGC
GGGCTGGCCGGCTTGGCGTGGCAAATTCGGGGATGTACTTGTATCCCGAAACCGACATGCCGAGTATTGAAGACGTCATC
AACCAAATGCAGCCGGATTACGTTGTGATCGACTCCGTTCAAACTATGAATGTGCCAGAAATGAATTCAGCGGTCGGTTC
TGTGGCACAGATTCGTGAAGTGACGGCAGAACTGATGCGCATTGCTAAGTCCAAAGGGGTGACCATTTTCATCGTTGGCC
ATGTCACTAAGGAAGGGGCGATTGCCGGGCCGAAGATTCTTGAGCATATGGTCGACACGGTGCTCTATTTTGAAGGCGAC
ATGCACCACACGTATCGAATCTTAAGATCCGTCAAAAATCGATTTGGATCGACCAATGAAATCGGCATTTTCGAAATGCA
TCAAGACGGGTTACAGGAAGTCGCCAATCCCTCGGAAATCTTCTTGGAAGAACGCTTGGCGGGGGCAACCGGTTCTGCGG
TGGTTGTTTCGATGGAAGGCACCCGACCAATCTTAGTCGAGATTCAAGCCTTGATCAGTCCAACGATGTATGGCAATGCT
AAACGGACGAGCAGTGGGTTGGATCACAATCGAGTGAGCTTGATCATGGCCGTTCTTGAGAAGCGGGCTAACTTGATGTT
GCAAAATCAGGACGCCTATCTCAAGGCAACAGGCGGTGTGAAACTCGATGAGCCGGCGATTGACCTCGCCATGGCTGTGG
CAATTGCGTCATCTTATCGAGATAAGGAGATTTCACCGACAGATTGTTTTGTCGGTGAAATCGGATTGACGGGGGAAGTG
CGCCGCGTCAATCGGATCGAAGAACGTGTCAAAGAGGCGGCCAAATTGGGTTTCAAACGGATTTTTGTACCCCGTAACAA
CTTGCAAGGCTGGCATGCGCCAAAAGATATTCAAGTCATTGGTGTTACAAGCATTGCCGAAGCGCTGCATAAGGTCTTTA
ATTAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(71-219)

Subunit ChlI of Mg-chelatase (ChlI)

(363-427)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

68.433

99.78

0.683
  radA Streptococcus pneumoniae Rx1

68.433

99.78

0.683
  radA Streptococcus pneumoniae D39

68.433

99.78

0.683
  radA Streptococcus pneumoniae R6

68.433

99.78

0.683
  radA Streptococcus pneumoniae TIGR4

68.433

99.78

0.683
  radA Streptococcus mitis SK321

68.212

99.78

0.681
  radA Bacillus subtilis subsp. subtilis str. 168

64.537

100

0.645

Multiple sequence alignment