Detailed information NTG0164055

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: AU077_RS06715
• Genome accession: NZ_CP013688
• Coordinates: 1356087..1356752 (-)
• Length: 221 a.a.
• NCBI ID: WP_039670586.1
• Uniprot ID: A0A380K4J8
• Organism: Streptococcus gallolyticus strain ICDDRB-NRC-S1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1351087..1361752

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AU077_RS06695 (AU077_06695)	-	1351967..1352383 (-)	417	WP_015057859.1	hypothetical protein	-
AU077_RS06700 (AU077_06700)	-	1352398..1353510 (-)	1113	WP_015057860.1	recombinase family protein	-
AU077_RS06705 (AU077_06705)	-	1353639..1354274 (-)	636	WP_058621817.1	CpXC domain-containing protein	-
AU077_RS06710 (AU077_06710)	-	1354427..1356094 (-)	1668	Protein_1375	ComEC/Rec2 family competence protein	-
AU077_RS06715 (AU077_06715)	comEA	1356087..1356752 (-)	666	WP_039670586.1	helix-hairpin-helix domain-containing protein	Machinery gene
AU077_RS06720 (AU077_06720)	-	1356847..1357593 (-)	747	WP_014294206.1	lysophospholipid acyltransferase family protein	-
AU077_RS06725 (AU077_06725)	-	1357701..1358462 (+)	762	WP_014294205.1	tRNA1(Val) (adenine(37)-N6)-methyltransferase	-
AU077_RS06730 (AU077_06730)	-	1358437..1358709 (+)	273	WP_014294204.1	GIY-YIG nuclease family protein	-
AU077_RS06735 (AU077_06735)	-	1358752..1360332 (-)	1581	WP_058621818.1	DEAD/DEAH box helicase	-
AU077_RS06740 (AU077_06740)	-	1360927..1361355 (-)	429	WP_039670333.1	DUF1492 domain-containing protein	-

Sequence

Protein

• Length: 221 a.a.
• Molecular weight: 23662.45 Da
• Isoelectric Point: 4.3842

>NTDB_id=164055 AU077_RS06715 WP_039670586.1 1356087..1356752(-) (comEA) [Streptococcus gallolyticus strain ICDDRB-NRC-S1]
MIEEIKEKLLENKTLVAMLGTILVMLIGFFAWSNVTKTTVDAQNDLPALSTTFSASSSASDLKTERSSSQTETQKVFVDI
KGAVKNEGVYELSNGRRVTDVVKLAGGFTEDADKKSVNLAEKVTDESMIYVARVGENVAPATTNSQVNGSAQQEESSDKI
NLNTATLAELQTISGIGAKRAQDIIDYRDANGGFSSVDDLANVSGIGEKTLEKLKSEVTVD

Nucleotide

• Length: 666 bp

>NTDB_id=164055 AU077_RS06715 WP_039670586.1 1356087..1356752(-) (comEA) [Streptococcus gallolyticus strain ICDDRB-NRC-S1]
ATGATTGAAGAAATAAAAGAAAAACTTTTAGAGAACAAAACGTTGGTAGCGATGTTGGGAACAATACTTGTCATGTTAAT
CGGATTTTTTGCTTGGTCAAACGTGACAAAAACAACAGTGGATGCACAAAATGACTTACCTGCCCTAAGCACGACTTTTT
CGGCAAGTAGCAGCGCAAGTGATTTAAAAACAGAACGTTCAAGTTCGCAAACTGAAACTCAAAAAGTGTTTGTAGATATC
AAAGGTGCTGTGAAAAATGAGGGTGTTTATGAGCTTTCAAACGGTAGGCGTGTGACAGATGTGGTTAAATTAGCAGGTGG
TTTTACAGAAGATGCTGATAAGAAGTCGGTGAATTTGGCTGAAAAAGTAACCGATGAATCAATGATTTATGTGGCAAGAG
TTGGTGAAAATGTTGCTCCTGCAACGACTAATTCACAAGTAAACGGTAGCGCTCAGCAGGAAGAAAGTTCAGATAAAATC
AATCTTAATACCGCAACTTTGGCAGAACTCCAGACGATTTCTGGCATTGGCGCCAAACGTGCCCAAGACATTATTGACTA
TCGTGATGCCAATGGTGGTTTTTCATCAGTTGATGACTTGGCGAATGTGTCTGGCATCGGAGAAAAAACACTAGAAAAGC
TAAAATCTGAGGTGACCGTTGATTAA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (78-131)

• Helix-hairpin-helix motif (HHH_3) (157-219)

3D structure

• AlphaFold DB: A0A380K4J8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Streptococcus thermophilus LMD-9	47.639	100	0.502
comEA/celA/cilE	Streptococcus mitis SK321	47.059	100	0.471
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	45.909	99.548	0.457
comEA/celA/cilE	Streptococcus mitis NCTC 12261	45.455	99.548	0.452
comEA/celA/cilE	Streptococcus pneumoniae Rx1	44.545	99.548	0.443
comEA/celA/cilE	Streptococcus pneumoniae D39	44.545	99.548	0.443
comEA/celA/cilE	Streptococcus pneumoniae R6	44.545	99.548	0.443
comEA	Latilactobacillus sakei subsp. sakei 23K	38.813	99.095	0.385