Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   WJ16_RS14235 Genome accession   NZ_CP013401
Coordinates   3153903..3155408 (+) Length   501 a.a.
NCBI ID   WP_069257603.1    Uniprot ID   -
Organism   Burkholderia metallica strain FL-6-5-30-S1-D7     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3148903..3160408
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WJ16_RS14210 (WJ16_14195) kdsD 3150147..3151130 (+) 984 WP_069257598.1 arabinose 5-phosphate isomerase KdsD -
  WJ16_RS14215 (WJ16_14200) - 3151130..3151666 (+) 537 WP_069257599.1 KdsC family phosphatase -
  WJ16_RS14220 (WJ16_14205) lptC 3151670..3152284 (+) 615 WP_069257600.1 LPS export ABC transporter periplasmic protein LptC -
  WJ16_RS14225 (WJ16_14210) lptA 3152305..3152970 (+) 666 WP_069257601.1 lipopolysaccharide transport periplasmic protein LptA -
  WJ16_RS14230 (WJ16_14215) lptB 3152967..3153743 (+) 777 WP_069257602.1 LPS export ABC transporter ATP-binding protein -
  WJ16_RS14235 (WJ16_14220) rpoN/rpoN1 3153903..3155408 (+) 1506 WP_069257603.1 RNA polymerase factor sigma-54 Machinery gene
  WJ16_RS14240 (WJ16_14225) hpf 3155567..3155926 (+) 360 WP_006477786.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  WJ16_RS14245 (WJ16_14230) - 3156122..3156634 (+) 513 WP_069257604.1 PTS sugar transporter subunit IIA -
  WJ16_RS14250 (WJ16_14235) hprK 3156764..3157732 (+) 969 WP_011353227.1 HPr(Ser) kinase/phosphatase -
  WJ16_RS14255 (WJ16_14240) rapZ 3157808..3158716 (+) 909 WP_069257605.1 RNase adapter RapZ -
  WJ16_RS14260 (WJ16_14245) - 3158744..3159382 (+) 639 WP_069257606.1 LON peptidase substrate-binding domain-containing protein -

Sequence

Protein


Download         Length: 501 a.a.        Molecular weight: 54744.79 Da        Isoelectric Point: 4.8711

>NTDB_id=162495 WJ16_RS14235 WP_069257603.1 3153903..3155408(+) (rpoN/rpoN1) [Burkholderia metallica strain FL-6-5-30-S1-D7]
MKASLQLRLSQHLALTPQLQQSIRLLQLSTLELQQEVAMAVAQNPLLENDDEWIASPLRVAADGSLIAQTPPASNTEPAA
TNGSSSSSDRNERDEPAGADEYDGYASGDGNDGPQWNLDEFGRSSGASDDDDLPPLQVQEAATSLRDHLSAQLRVTQASP
RDRALVMFLIESLDDDGYLTATLDEVLADLPPELEIDCDELNAALALLHSFDPAGVGARSASECLKLQLVRLDPSPTRTL
ALEIVSQHLELLAARDFTRLRKHLKASDDALRDAHALIRSLEPFPGAAYGRAEADYVVPDIIVKKAGQGWLAELNPDVVP
RLRINSLYANILRNSRGDPSAGSLKQQLQEARWLIKNIQQRFDTILRVAQAIVERQKNFFAHGEIAMRPLVLREIADTLG
LHESTVSRVTTGKYMLTPFGTLEFKYFFGSHVSTDTGGAASSTAIRALIKQLIGAEDPKSPLSDSRIAELLAEQGFVVAR
RTVAKYREALKIPAVNLRKSL

Nucleotide


Download         Length: 1506 bp        

>NTDB_id=162495 WJ16_RS14235 WP_069257603.1 3153903..3155408(+) (rpoN/rpoN1) [Burkholderia metallica strain FL-6-5-30-S1-D7]
ATGAAAGCCAGCCTTCAACTCCGCCTGTCGCAACATCTGGCGCTGACGCCCCAGCTACAGCAGTCGATCCGGCTCCTGCA
GTTGTCGACGCTCGAATTGCAGCAGGAAGTCGCGATGGCCGTCGCGCAGAACCCGCTGCTCGAGAACGACGACGAATGGA
TCGCGAGCCCGTTGCGGGTCGCGGCAGACGGATCGCTGATCGCGCAGACACCGCCCGCATCGAACACCGAGCCCGCCGCG
ACGAACGGCAGCAGTTCGTCGAGCGATCGCAACGAGCGCGACGAGCCGGCCGGCGCGGATGAATACGACGGTTACGCGTC
CGGCGACGGCAACGACGGCCCGCAGTGGAACCTCGATGAATTCGGCCGCTCGAGCGGCGCGTCCGACGACGACGACCTCC
CGCCGCTGCAGGTACAGGAAGCGGCGACGTCGCTGCGCGACCATCTGTCCGCGCAGTTGCGCGTCACGCAGGCCAGCCCG
CGCGATCGCGCGCTCGTGATGTTCCTGATCGAGTCGCTCGACGACGACGGCTACCTGACCGCGACGCTCGACGAAGTGCT
CGCCGACCTGCCGCCCGAGCTGGAAATCGACTGCGACGAACTGAACGCGGCGCTCGCGCTGCTGCACAGCTTCGACCCGG
CCGGCGTCGGCGCCCGCTCGGCCTCCGAATGCCTGAAGCTGCAGCTGGTGCGCCTCGATCCGTCCCCGACACGCACGCTC
GCGCTCGAGATCGTGTCGCAGCATCTCGAACTGCTCGCCGCCCGCGATTTCACGCGGCTGCGCAAGCACCTGAAGGCGAG
CGACGACGCGCTGCGCGACGCGCACGCGCTGATCCGCTCGCTGGAGCCGTTCCCCGGCGCCGCCTACGGCAGGGCCGAGG
CCGACTACGTCGTGCCCGACATCATCGTGAAGAAGGCCGGCCAGGGATGGCTCGCGGAGCTCAATCCGGACGTCGTGCCG
CGCCTGCGGATCAACAGCCTCTACGCGAACATCCTGCGCAACAGCCGCGGCGATCCGTCGGCCGGTTCGCTCAAGCAACA
GCTGCAGGAAGCACGCTGGCTGATCAAGAACATCCAGCAGCGATTCGACACGATCCTGCGTGTCGCGCAGGCCATTGTCG
AGCGTCAGAAGAACTTCTTTGCGCACGGTGAAATTGCCATGCGCCCCTTGGTTTTGCGGGAAATAGCTGATACGCTGGGC
CTACACGAGTCGACTGTCAGCCGTGTGACAACCGGGAAGTACATGCTGACCCCGTTCGGGACGCTTGAATTTAAGTACTT
CTTCGGATCTCACGTTTCGACCGACACCGGTGGCGCCGCTTCGTCGACCGCCATCCGAGCCCTCATCAAGCAACTGATAG
GAGCTGAAGACCCAAAATCGCCACTTTCGGACAGCCGCATTGCCGAGCTGCTGGCAGAACAGGGTTTCGTGGTCGCACGC
CGCACGGTTGCGAAATATCGCGAAGCCCTCAAGATCCCGGCAGTGAATCTGCGCAAGTCTCTTTAA

Domains

Predicted by InterProScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(137-328)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(344-499)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

68.775

100

0.695

Multiple sequence alignment