Detailed information NTG0144491

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: AWM80_RS01690
• Genome accession: NZ_CP014166
• Coordinates: 304427..305548 (+)
• Length: 373 a.a.
• NCBI ID: WP_003246478.1
• Uniprot ID: O34327
• Organism: Bacillus subtilis subsp. subtilis strain CU1050
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 299427..310548

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
AWM80_RS01670 (AWM80_01675)	ycdA	299435..300499 (-)	1065	WP_003234750.1	DUF4352 domain-containing protein	-
AWM80_RS21535	-	300522..300653 (-)	132	WP_003246334.1	hypothetical protein	-
AWM80_RS01675 (AWM80_01680)	ycdB	300827..302245 (+)	1419	WP_003246352.1	YcdB/YcdC domain-containing protein	-
AWM80_RS01680 (AWM80_01685)	ycdC	302432..303793 (+)	1362	WP_010886397.1	YcdB/YcdC domain-containing protein	-
AWM80_RS01685 (AWM80_01690)	cwlK	303801..304304 (-)	504	WP_009966473.1	peptidoglycan L-alanyl-D-glutamate endopeptidase CwlK	-
AWM80_RS01690 (AWM80_01695)	rapF	304427..305548 (+)	1122	WP_003246478.1	response regulator aspartate phosphatase RapJ	Regulator
AWM80_RS01695 (AWM80_01700)	ycdF	305655..306431 (+)	777	WP_003246415.1	SDR family oxidoreductase	-
AWM80_RS01700 (AWM80_01705)	ycdG	306456..308141 (+)	1686	WP_003234736.1	glycoside hydrolase family 13 protein	-
AWM80_RS01705 (AWM80_01710)	znuA	308329..309288 (+)	960	WP_003234733.1	metal ABC transporter substrate-binding protein	-
AWM80_RS01710 (AWM80_01715)	znuC	309344..310039 (+)	696	WP_003234730.1	zinc ABC transporter ATP-binding protein ZnuC	-

Sequence

Protein

• Length: 373 a.a.
• Molecular weight: 44414.31 Da
• Isoelectric Point: 4.8371

>NTDB_id=144491 AWM80_RS01690 WP_003246478.1 304427..305548(+) (rapF) [Bacillus subtilis subsp. subtilis strain CU1050]
MRAKIPSEEVAVKLNEWYKLIRAFEADQAEALKQEIEYDLEDMEENQDLLLYFSLMEFRHRIMLDKLMPVKDSDTKPPFS
DMLNEIESNQQKLTGLLEYYFYYFRGMYEFKQKNFILAIDHYKHAEEKLEYVEDEIEKAEFLFKVAEVYYHIKQTYFSMN
YASQALDIYTKYELYGRRRVQCEFIIAGNLTDVYHHEKALTHLCSALEHARQLEEAYMIAAAYYNVGHCKYSLGDYKEAE
GYFKTAAAIFEEHNFQQAVQAVFSLTHIYCKEGKYDKAVEAYDRGIKSAAEWEDDMYLTKFRLIHELYLGSGDLNVLTEC
FDLLESRQLLADAEDLLHDTAERFNQLEHYESAAFFYRRLMNIKKKLAEQRFQ

Nucleotide

• Length: 1122 bp

>NTDB_id=144491 AWM80_RS01690 WP_003246478.1 304427..305548(+) (rapF) [Bacillus subtilis subsp. subtilis strain CU1050]
ATGAGAGCAAAGATTCCATCAGAAGAAGTAGCAGTAAAGCTGAATGAATGGTACAAGCTCATTCGCGCATTTGAAGCAGA
TCAAGCAGAAGCGTTAAAGCAGGAGATTGAATATGATTTAGAAGATATGGAAGAGAATCAGGACTTGCTTTTATATTTTT
CTTTGATGGAATTCCGGCATCGAATCATGCTTGATAAGCTGATGCCGGTGAAAGACAGCGATACCAAGCCTCCGTTCTCT
GATATGCTGAACGAAATTGAAAGCAATCAGCAAAAACTCACGGGCTTACTGGAATACTACTTTTATTATTTCAGAGGAAT
GTACGAATTCAAGCAGAAAAATTTCATATTAGCGATTGACCATTATAAACATGCCGAGGAAAAGCTCGAGTATGTCGAGG
ATGAGATCGAAAAAGCTGAGTTTCTTTTTAAAGTCGCAGAAGTGTATTATCACATCAAACAAACGTATTTTTCCATGAAT
TATGCGAGCCAGGCGCTTGATATCTATACGAAATATGAACTGTACGGTCGCCGCCGGGTTCAGTGTGAATTTATCATCGC
CGGAAATTTGACCGATGTGTATCATCATGAAAAAGCACTGACCCACTTGTGCAGCGCTTTAGAACATGCCAGGCAGCTTG
AAGAAGCGTACATGATCGCTGCTGCCTATTATAATGTCGGACACTGTAAGTACAGTCTGGGTGATTACAAAGAAGCGGAG
GGCTATTTCAAAACAGCCGCCGCCATTTTTGAGGAGCACAACTTTCAGCAAGCTGTTCAAGCTGTATTTTCACTGACCCA
TATCTACTGTAAAGAGGGAAAATACGACAAGGCAGTAGAGGCTTATGACCGCGGGATAAAGAGCGCGGCTGAATGGGAGG
ATGACATGTACTTGACGAAATTCCGCCTCATTCATGAGCTGTATCTTGGAAGCGGCGACCTGAACGTGCTTACAGAATGC
TTTGACCTGCTGGAATCCAGGCAGCTTCTTGCTGACGCCGAAGATCTGCTGCATGATACAGCGGAACGTTTCAATCAGTT
GGAACATTACGAGTCCGCCGCTTTCTTTTACCGCAGGCTGATGAATATTAAAAAGAAGCTTGCCGAGCAGCGTTTTCAAT
AG

Domains

Predicted by InterProScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (196-252)

3D structure

• PDB: 4GYO

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	42.391	98.66	0.418
rapC	Bacillus subtilis subsp. subtilis str. 168	41.257	98.123	0.405