Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYB   Type   Machinery gene
Locus tag   SD89_RS00655 Genome accession   NZ_CP010449
Coordinates   102771..103805 (+) Length   344 a.a.
NCBI ID   WP_227875237.1    Uniprot ID   -
Organism   Streptococcus pyogenes strain NGAS322     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 97771..108805
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SD89_RS00645 (SD89_00645) - 101439..101804 (+) 366 WP_002986560.1 DUF1033 family protein -
  SD89_RS00650 (SD89_00650) comYA 101897..102835 (+) 939 WP_002993471.1 competence type IV pilus ATPase ComGA Machinery gene
  SD89_RS00655 (SD89_00655) comYB 102771..103805 (+) 1035 WP_227875237.1 competence type IV pilus assembly protein ComGB Machinery gene
  SD89_RS00660 (SD89_00660) comGC 103807..104133 (+) 327 WP_053308148.1 competence type IV pilus major pilin ComGC Machinery gene
  SD89_RS00665 (SD89_00665) comGD 104108..104536 (+) 429 WP_002986548.1 competence type IV pilus minor pilin ComGD -
  SD89_RS00670 (SD89_00670) comGE 104493..104777 (+) 285 WP_011284422.1 competence type IV pilus minor pilin ComGE -
  SD89_RS00675 (SD89_00675) comYF 104770..105204 (+) 435 WP_002992738.1 competence type IV pilus minor pilin ComGF Machinery gene
  SD89_RS00680 (SD89_00680) comGG 105188..105514 (+) 327 WP_010921801.1 competence type IV pilus minor pilin ComGG -
  SD89_RS00685 (SD89_00685) comYH 105612..106565 (+) 954 WP_053308149.1 class I SAM-dependent methyltransferase Machinery gene
  SD89_RS00690 (SD89_00690) - 106624..107820 (+) 1197 WP_002987791.1 acetate kinase -
  SD89_RS00695 (SD89_00695) - 108006..108314 (+) 309 Protein_91 hypothetical protein -

Sequence

Protein


Download         Length: 344 a.a.        Molecular weight: 39692.15 Da        Isoelectric Point: 9.7085

>NTDB_id=137311 SD89_RS00655 WP_227875237.1 102771..103805(+) (comYB) [Streptococcus pyogenes strain NGAS322]
MISFLRQDISIPSKLSLKKLSSKHQHKFIQLLANLLSTGFSFAEVIAFLKRSQLLQLDYVLKMEESLLKGQGLADMLSGL
GFSDAILTQISLADRHGNIETTLVAIQHYLNQMARIRRKTVEVITYPLILLLFLFVMMLGLRRYLVPQLETQNQITYFLN
HFPAFFIGFCSGLILLFGMVWLRWRSQSRLKLYSRLSRYPFLGKLLKQYLTSYYSREWGTLIGQGLDLMTILDIMAIEKS
SLMKELAEDIRMSLLEGQAFHIKVATYPFFKKELSLMIEYGEIKSKLGAELEIYAQESWEQFFSQLYQVTQLIQPAIFLV
VAVTIVMIYAAILLPIYQNMGGIF

Nucleotide


Download         Length: 1035 bp        

>NTDB_id=137311 SD89_RS00655 WP_227875237.1 102771..103805(+) (comYB) [Streptococcus pyogenes strain NGAS322]
TTGATCAGCTTCTTGAGGCAGGACATCTCAATCCCAAGCAAGCTAAGCTTGAAAAAATTATCTAGTAAACATCAGCATAA
ATTCATTCAATTACTAGCTAATCTTTTATCTACAGGATTTAGCTTCGCAGAGGTTATTGCTTTTTTAAAAAGAAGCCAGT
TGCTTCAGCTAGATTATGTTCTTAAAATGGAAGAGTCTTTATTAAAAGGACAAGGCTTAGCAGATATGTTGTCGGGTTTA
GGTTTTTCAGATGCTATTCTTACTCAAATTAGTTTAGCTGATAGACACGGTAATATTGAAACAACATTAGTAGCGATTCA
ACATTACTTGAACCAAATGGCAAGGATCAGACGAAAAACTGTTGAAGTTATCACCTATCCTCTTATTCTTTTACTTTTTC
TTTTTGTGATGATGCTAGGGTTGAGACGCTATTTAGTCCCTCAATTAGAAACTCAAAATCAGATAACTTACTTTCTTAAC
CATTTTCCTGCCTTCTTTATAGGTTTTTGCTCAGGTCTTATTTTGTTGTTCGGGATGGTATGGCTACGATGGCGATCTCA
AAGCCGCTTGAAACTCTATAGTCGGCTAAGTCGTTATCCTTTTTTAGGTAAACTGTTAAAACAATACTTAACTAGTTATT
ATTCTAGAGAATGGGGTACGTTAATAGGACAAGGTCTTGATTTGATGACTATTTTAGACATTATGGCCATTGAGAAATCC
TCACTAATGAAAGAATTAGCTGAGGACATCAGAATGAGTCTGCTTGAAGGGCAAGCCTTTCACATTAAAGTAGCTACCTA
CCCTTTTTTTAAAAAAGAATTAAGTTTAATGATTGAATACGGTGAAATCAAATCGAAACTGGGGGCTGAGTTGGAAATCT
ATGCCCAGGAAAGCTGGGAACAATTTTTTAGTCAGCTTTATCAAGTGACACAACTTATTCAACCAGCTATTTTTTTAGTA
GTTGCTGTGACAATCGTCATGATTTATGCGGCAATCCTATTACCAATTTACCAAAATATGGGAGGTATTTTTTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(215-335)

Type II secretion system (T2SS), protein F (T2SSF)

(28-147)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYB Streptococcus mutans UA140

53.687

98.547

0.529
  comYB Streptococcus mutans UA159

53.687

98.547

0.529
  comYB Streptococcus gordonii str. Challis substr. CH1

50.294

98.837

0.497
  comGB/cglB Streptococcus mitis NCTC 12261

47.321

97.674

0.462
  comGB/cglB Streptococcus mitis SK321

47.447

96.802

0.459
  comGB/cglB Streptococcus pneumoniae Rx1

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae D39

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae R6

45.345

96.802

0.439
  comGB/cglB Streptococcus pneumoniae TIGR4

45.345

96.802

0.439
  comGB Lactococcus lactis subsp. cremoris KW2

43.077

94.477

0.407

Multiple sequence alignment