Detailed information NTG0136782

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: RU84_RS13730
• Genome accession: NZ_CP010397
• Coordinates: 2937840..2938958 (+)
• Length: 372 a.a.
• NCBI ID: WP_000347036.1
• Uniprot ID: A0A009PAD6
• Organism: Acinetobacter baumannii strain 6200
• Function: mediate the depolymerization of the type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2932840..2943958

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
RU84_RS13720 (RU84_13720)	-	2935956..2936648 (-)	693	WP_032050220.1	YggS family pyridoxal phosphate-dependent enzyme	-
RU84_RS13725 (RU84_13725)	pilT	2936775..2937812 (+)	1038	WP_000355489.1	type IV pilus twitching motility protein PilT	Machinery gene
RU84_RS13730 (RU84_13730)	pilU	2937840..2938958 (+)	1119	WP_000347036.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
RU84_RS13735 (RU84_13735)	fur	2939028..2939465 (-)	438	WP_001122847.1	ferric iron uptake transcriptional regulator	-
RU84_RS13740 (RU84_13740)	-	2939577..2939975 (+)	399	WP_001170994.1	outer membrane protein assembly factor BamE	-
RU84_RS13745 (RU84_13745)	-	2940030..2940353 (-)	324	WP_000442589.1	RnfH family protein	-
RU84_RS13750 (RU84_13750)	-	2940353..2941420 (-)	1068	WP_031962994.1	membrane protein	-
RU84_RS13755 (RU84_13755)	-	2941573..2942028 (-)	456	WP_000782976.1	bacteriohemerythrin	-
RU84_RS13760 (RU84_13760)	-	2942281..2943129 (-)	849	WP_000336554.1	class II glutamine amidotransferase	-

Sequence

Protein

• Length: 372 a.a.
• Molecular weight: 41905.11 Da
• Isoelectric Point: 6.6944

>NTDB_id=136782 RU84_RS13730 WP_000347036.1 2937840..2938958(+) (pilU) [Acinetobacter baumannii strain 6200]
MDFNDLLNLMVEKKSSDLFITDGVAPSMKINGQIVPISKNSLSGEVIGQLLHSIMSEKQRKEFAETRECNFAIMNREKTA
RFRVSAFQQRDMPGMVLRRIETKIPSIDDLQLPPVLKDLSMTKRGIIIFVGATGTGKSTSLASMISHRNHNSKGHIITIE
DPIEFIHEHAGCIITQREVGIDTDSFEIALKNTLRQAPDVILIGEIRSREVMDYAIGFAETGHLVLATMHANNANQALDR
IIHFFESDRHSQLYMDLSLNLKAMIAQQLIPTPDGNSRRAAIEILINSPLISDYIRKGEIHEIKDLMKRSRELGMQTFDQ
ALFDLYKAGQITYKDALKHADSPNDLRLTIKLADEGPDQLSDGKQHLTFDRQ

Nucleotide

• Length: 1119 bp

>NTDB_id=136782 RU84_RS13730 WP_000347036.1 2937840..2938958(+) (pilU) [Acinetobacter baumannii strain 6200]
ATGGATTTTAATGACTTGCTCAACCTGATGGTTGAAAAAAAATCATCGGATCTTTTTATTACAGATGGCGTTGCGCCTTC
TATGAAGATTAACGGGCAAATTGTTCCAATTTCAAAAAATAGTCTTTCTGGGGAAGTGATTGGTCAACTGTTACATTCCA
TCATGAGTGAAAAACAACGCAAAGAATTTGCAGAAACTCGTGAATGTAACTTTGCCATTATGAACCGTGAAAAAACGGCG
CGTTTTCGTGTCAGTGCTTTTCAGCAGCGCGACATGCCAGGCATGGTACTACGTCGTATTGAAACCAAGATTCCTTCAAT
TGATGACTTGCAGTTACCGCCTGTACTTAAAGATTTATCGATGACCAAACGCGGCATTATTATTTTCGTAGGCGCGACAG
GTACAGGTAAATCCACTTCATTGGCTTCAATGATCAGCCATCGTAACCATAACTCTAAGGGCCATATCATTACCATTGAA
GACCCGATTGAGTTTATTCACGAACATGCAGGTTGCATCATTACTCAGCGTGAAGTCGGGATCGATACCGACTCATTTGA
AATTGCCTTGAAAAATACACTACGACAAGCGCCGGATGTGATCTTAATTGGTGAGATCCGTTCTCGTGAAGTCATGGACT
ATGCGATTGGCTTTGCTGAAACAGGTCACCTTGTATTAGCTACCATGCACGCCAACAACGCCAACCAAGCACTCGACCGT
ATTATTCACTTTTTTGAGAGTGACCGTCATAGTCAGCTTTACATGGACTTGTCACTTAACTTAAAGGCAATGATTGCGCA
GCAGCTCATTCCAACACCAGATGGTAATTCACGCCGTGCAGCAATTGAGATTTTAATTAACTCACCATTAATTTCAGACT
ACATCCGTAAAGGTGAAATTCATGAAATTAAAGATTTGATGAAACGCTCACGTGAACTTGGTATGCAGACCTTTGACCAA
GCTTTATTTGATTTATATAAAGCTGGTCAAATTACCTACAAAGATGCGCTTAAACATGCTGATTCACCGAACGATTTGCG
TTTAACCATTAAACTTGCAGATGAAGGTCCCGATCAATTATCAGACGGGAAACAGCATTTAACTTTTGATCGTCAGTAA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (31-270)

3D structure

• AlphaFold DB: A0A009PAD6

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Acinetobacter baylyi ADP1	75.926	100	0.772
pilU	Pseudomonas stutzeri DSM 10701	69.293	98.925	0.685
pilU	Vibrio cholerae strain A1552	53.243	99.462	0.53
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	42.197	93.011	0.392