Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   ACJ74_RS05650 Genome accession   NZ_CP011915
Coordinates   1135403..1136899 (+) Length   498 a.a.
NCBI ID   WP_001040641.1    Uniprot ID   -
Organism   Escherichia coli strain PSUO2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1130403..1141899
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ74_RS05620 (ACJ74_05445) tusA 1130483..1130728 (+) 246 WP_000130621.1 sulfurtransferase TusA -
  ACJ74_RS05625 (ACJ74_05450) zntA 1130997..1133195 (-) 2199 WP_000106604.1 Zn(II)/Cd(II)/Pb(II) translocating P-type ATPase ZntA -
  ACJ74_RS05630 (ACJ74_05455) yhhN 1133269..1133895 (-) 627 WP_000964718.1 lysoplasmalogenase -
  ACJ74_RS05635 (ACJ74_05460) yhhM 1134036..1134395 (+) 360 WP_000042904.1 DUF2500 domain-containing protein -
  ACJ74_RS05640 (ACJ74_05465) yhhL 1134398..1134667 (-) 270 WP_001295207.1 DUF1145 family protein -
  ACJ74_RS05645 (ACJ74_05470) rsmD 1134657..1135253 (-) 597 WP_000743193.1 16S rRNA (guanine(966)-N(2))-methyltransferase -
  ACJ74_RS05650 (ACJ74_05475) pilA 1135403..1136899 (+) 1497 WP_001040641.1 signal recognition particle-docking protein FtsY Machinery gene
  ACJ74_RS05655 (ACJ74_05480) ftsE 1136902..1137570 (+) 669 WP_000617723.1 cell division ATP-binding protein FtsE -
  ACJ74_RS05660 (ACJ74_05485) ftsX 1137563..1138621 (+) 1059 WP_001042003.1 permease-like cell division protein FtsX -
  ACJ74_RS05665 (ACJ74_05490) rpoH 1138866..1139720 (+) 855 WP_000130217.1 RNA polymerase sigma factor RpoH -
  ACJ74_RS05670 (ACJ74_05495) livJ 1139991..1141094 (+) 1104 WP_001350438.1 branched chain amino acid ABC transporter substrate-binding protein LivJ -
  ACJ74_RS05675 (ACJ74_05505) panM 1141282..1141665 (-) 384 WP_000778808.1 aspartate 1-decarboxylase autocleavage activator PanM -

Sequence

Protein


Download         Length: 498 a.a.        Molecular weight: 54614.37 Da        Isoelectric Point: 4.1733

>NTDB_id=129803 ACJ74_RS05650 WP_001040641.1 1135403..1136899(+) (pilA) [Escherichia coli strain PSUO2]
MAKEKKRGFFSWLGFGQKEQTPEKETEVQNEQPVVEEIVQAQEPVKASEHAVEEQPQAHTEAEAETFAADVVEVTEQVAE
SEKAQPEAEVVAQPEPVVEETPEPVAIEREELPLSEDVNAEAVSPEEWQAEAETVEIVEAAEEEAAKEEITDEEPEAQAL
AAEAAEEAVMVVSPAEEEQPVEEIAQEQEKPTKEGFFARLKRSLLKTKENLGSGFISLFRGKKIDDDLFEELEEQLLIAD
VGVETTRKIITNLTEGASRKQLRDAEALYGLLKEEMGEILAKVDEPLNVEGKTPFVILMVGVNGVGKTTTIGKLARQFEQ
QGKSVMLAAGDTFRAAAVEQLQVWGQRNNIPVIAQHTGADSASVIFDAIQAAKARNIDVLIADTAGRLQNKSHLMEELKK
IVRVMKKLDVEAPHEVMLTIDASTGQNAVSQAKLFHEAVGLTGITLTKLDGTAKGGVIFSVADQFGIPIRYIGVGERIED
LRPFKADDFIEALFARED

Nucleotide


Download         Length: 1497 bp        

>NTDB_id=129803 ACJ74_RS05650 WP_001040641.1 1135403..1136899(+) (pilA) [Escherichia coli strain PSUO2]
ATGGCGAAAGAAAAAAAACGTGGCTTTTTTTCCTGGCTGGGCTTTGGTCAAAAAGAGCAGACCCCGGAAAAAGAGACAGA
AGTTCAGAATGAACAACCAGTTGTAGAAGAAATCGTTCAGGCGCAAGAGCCTGTGAAGGCTTCTGAACACGCCGTTGAAG
AGCAGCCGCAGGCGCATACTGAAGCCGAGGCGGAAACTTTTGCTGCCGACGTTGTGGAAGTTACTGAACAGGTTGCTGAA
AGTGAAAAAGCGCAGCCTGAAGCGGAAGTCGTTGCACAGCCGGAACCGGTCGTAGAAGAAACGCCGGAGCCAGTGGCTAT
CGAACGTGAAGAACTGCCGTTGTCGGAAGACGTCAACGCCGAAGCGGTTTCGCCAGAAGAGTGGCAGGCCGAAGCGGAAA
CCGTAGAGATTGTCGAAGCGGCGGAAGAAGAAGCGGCTAAAGAAGAAATCACCGACGAAGAGCCGGAGGCACAGGCGCTG
GCTGCCGAAGCGGCAGAAGAGGCGGTAATGGTGGTTTCTCCGGCAGAAGAAGAGCAGCCGGTGGAAGAAATCGCTCAGGA
GCAGGAAAAACCGACCAAAGAAGGTTTCTTCGCGCGCCTGAAACGCAGCCTGTTAAAAACCAAAGAAAATCTCGGTTCCG
GATTTATCAGCCTGTTCCGCGGTAAAAAAATCGACGATGATCTGTTTGAAGAGCTGGAAGAACAGCTGTTGATCGCCGAT
GTGGGCGTGGAAACCACTCGTAAAATTATCACCAATCTGACGGAAGGCGCATCCCGCAAGCAGCTTCGTGATGCCGAGGC
GCTCTATGGCCTGCTGAAAGAAGAGATGGGCGAGATTCTGGCGAAAGTCGATGAGCCGCTGAATGTTGAAGGTAAAACGC
CGTTCGTGATCCTGATGGTGGGCGTCAACGGTGTGGGTAAAACCACGACGATTGGTAAGCTGGCGCGTCAGTTTGAGCAG
CAGGGTAAATCGGTGATGCTGGCGGCAGGCGATACTTTCCGTGCAGCAGCGGTTGAACAGCTTCAGGTCTGGGGTCAGCG
CAACAATATTCCGGTGATTGCCCAGCATACCGGTGCGGATTCCGCTTCTGTTATCTTCGATGCCATTCAGGCCGCTAAAG
CGCGTAACATCGACGTCCTGATTGCCGATACCGCCGGACGCCTGCAAAACAAATCGCACCTGATGGAAGAGTTGAAGAAA
ATCGTCCGCGTGATGAAGAAACTCGACGTTGAAGCTCCGCATGAAGTTATGCTGACCATCGATGCCAGCACCGGGCAGAA
TGCGGTAAGCCAGGCCAAACTGTTCCACGAAGCCGTTGGCTTAACCGGCATCACGCTAACGAAACTGGACGGCACGGCGA
AAGGCGGGGTAATTTTCTCGGTGGCTGACCAGTTTGGTATCCCTATCCGCTACATTGGTGTCGGCGAACGTATTGAGGAT
TTGCGTCCGTTTAAGGCGGACGACTTTATAGAGGCACTTTTTGCCCGAGAGGATTAA

Domains

Predicted by InterProScan.

SRP54-type protein, GTPase domain (SRP54)

(294-494)

SRP54-type protein, helical bundle domain (SRP54_N)

(211-276)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Neisseria gonorrhoeae MS11

48.831

77.309

0.378