Detailed information    

insolico Bioinformatically predicted

Overview

Name   radC   Type   Machinery gene
Locus tag   ACJ74_RS04675 Genome accession   NZ_CP011915
Coordinates   934815..935483 (+) Length   222 a.a.
NCBI ID   WP_001297375.1    Uniprot ID   P65957
Organism   Escherichia coli strain PSUO2     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 929815..940483
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACJ74_RS04640 (ACJ74_04510) yicC 929834..930697 (-) 864 WP_000621332.1 YicC/YloC family endoribonuclease -
  ACJ74_RS04650 (ACJ74_04515) rph 930824..931540 (+) 717 WP_001247094.1 ribonuclease PH -
  ACJ74_RS04655 (ACJ74_04520) pyrE 931606..932247 (+) 642 WP_000806177.1 orotate phosphoribosyltransferase -
  ACJ74_RS04660 (ACJ74_04525) slmA 932284..932880 (-) 597 WP_000818603.1 nucleoid occlusion factor SlmA -
  ACJ74_RS04665 (ACJ74_04530) dut 932987..933445 (-) 459 WP_000976070.1 dUTP diphosphatase -
  ACJ74_RS04670 (ACJ74_04535) coaBC 933423..934643 (-) 1221 WP_000050139.1 bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate--cysteine ligase CoaBC -
  ACJ74_RS04675 (ACJ74_04540) radC 934815..935483 (+) 669 WP_001297375.1 RadC family protein Machinery gene
  ACJ74_RS04680 (ACJ74_04545) rpmB 935700..935936 (+) 237 WP_000091955.1 50S ribosomal protein L28 -
  ACJ74_RS04685 (ACJ74_04550) rpmG 935957..936124 (+) 168 WP_001051798.1 50S ribosomal protein L33 -
  ACJ74_RS04690 (ACJ74_04555) mutM 936222..937031 (+) 810 WP_001114533.1 bifunctional DNA-formamidopyrimidine glycosylase/DNA-(apurinic or apyrimidinic site) lyase -
  ACJ74_RS04695 (ACJ74_04560) coaD 937070..937549 (-) 480 WP_001171873.1 pantetheine-phosphate adenylyltransferase -
  ACJ74_RS04700 (ACJ74_04565) waaA 937557..938834 (-) 1278 WP_000891574.1 lipid IV(A) 3-deoxy-D-manno-octulosonic acid transferase -
  ACJ74_RS04705 (ACJ74_04570) rfaQ 939247..940305 (+) 1059 WP_000360397.1 lipopolysaccharide core heptosyltransferase RfaQ -

Sequence

Protein


Download         Length: 222 a.a.        Molecular weight: 25258.35 Da        Isoelectric Point: 7.9748

>NTDB_id=129799 ACJ74_RS04675 WP_001297375.1 934815..935483(+) (radC) [Escherichia coli strain PSUO2]
MKNNAQLLMPREKMLKFGISALTDVELLALFLRTGTRGKDVLTLAKEMLENFGSLYGLLTSEYEQFSGVHGIGVAKFAQL
KGIAELARRYYNVRMREESPLLSPEMTREFLQSQLTGEEREIFMVIFLDSQHRVITHSRLFSGTLNHVEVHPREIIREAI
KINASALILAHNHPSGCAEPSKADKLITERIIKSCQFMDLRVLDHIVIGRGEYVSFAERGWI

Nucleotide


Download         Length: 669 bp        

>NTDB_id=129799 ACJ74_RS04675 WP_001297375.1 934815..935483(+) (radC) [Escherichia coli strain PSUO2]
GTGAAAAACAATGCACAGCTGTTGATGCCGCGCGAAAAAATGCTGAAGTTTGGTATTAGCGCCTTAACGGATGTCGAGCT
GTTGGCGCTATTTCTGCGTACCGGAACGCGCGGTAAAGATGTATTAACCCTGGCAAAAGAGATGCTGGAGAATTTCGGCT
CTCTTTATGGCTTGTTAACCTCTGAATATGAGCAATTTAGTGGCGTTCATGGAATTGGCGTGGCGAAATTCGCCCAGTTA
AAGGGGATTGCCGAACTGGCGCGGCGTTACTACAACGTACGGATGCGTGAAGAAAGCCCTTTACTCAGCCCGGAGATGAC
GAGGGAATTTTTACAAAGCCAGCTCACGGGCGAGGAGCGGGAGATCTTTATGGTGATCTTTCTCGACTCCCAACACAGAG
TTATAACGCATAGCCGTCTTTTTTCCGGCACGCTAAACCATGTTGAAGTCCATCCTCGGGAAATTATCCGCGAAGCGATA
AAAATAAACGCCTCGGCGCTGATCCTTGCGCATAATCACCCTTCGGGTTGTGCTGAACCCAGTAAAGCGGATAAACTCAT
TACTGAACGGATAATAAAGAGTTGTCAGTTCATGGATTTACGCGTGCTCGACCATATCGTGATTGGGCGTGGAGAGTATG
TTTCTTTTGCCGAACGCGGCTGGATTTAA

Domains

Predicted by InterProScan.

RadC-like JAB domain (RadC)

(103-220)

UPF0758 N-terminal (UPF0758_N)

(9-77)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB P65957

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radC Haemophilus influenzae Rd KW20

49.55

100

0.496

Multiple sequence alignment