Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   XTG_RS15505 Genome accession   NZ_LR994544
Coordinates   3651909..3653642 (-) Length   577 a.a.
NCBI ID   WP_181901227.1    Uniprot ID   -
Organism   Xanthomonas euroxanthea isolate X. euroxanthea CPBF 424     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3646909..3658642
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  XTG_RS15490 (XTG_003098) sucC 3647010..3648179 (-) 1170 WP_016901264.1 ADP-forming succinate--CoA ligase subunit beta -
  XTG_RS15495 (XTG_003099) - 3648411..3650024 (+) 1614 WP_104544483.1 HAMP domain-containing sensor histidine kinase -
  XTG_RS15500 (XTG_003100) pilR 3650217..3651611 (+) 1395 WP_180496733.1 sigma-54 dependent transcriptional regulator Regulator
  XTG_RS15505 (XTG_003101) pilB 3651909..3653642 (-) 1734 WP_181901227.1 type IV-A pilus assembly ATPase PilB Machinery gene
  XTG_RS20755 (XTG_003102) - 3653687..3654127 (-) 441 WP_115677261.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  XTG_RS15515 (XTG_003103) - 3654375..3654827 (-) 453 WP_115677262.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  XTG_RS15520 (XTG_003104) pilC 3655181..3656434 (+) 1254 WP_115677263.1 type II secretion system F family protein Machinery gene
  XTG_RS15525 (XTG_003105) - 3656441..3657304 (+) 864 WP_115677264.1 A24 family peptidase -
  XTG_RS15530 (XTG_003106) coaE 3657318..3657926 (+) 609 WP_115677265.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62623.80 Da        Isoelectric Point: 5.6078

>NTDB_id=1134995 XTG_RS15505 WP_181901227.1 3651909..3653642(-) (pilB) [Xanthomonas euroxanthea isolate X. euroxanthea CPBF 424]
MNAGSLNLVGITGIARRLVQDGVLEEVAARAAMDQASSAKVPLPQWFAEKKLVTASQLAAANAVEFGMPLMDVSVFDASQ
NAIKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLGD
DDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKVLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAPV
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDGSAAKLGIEKLGYEADQQKL
FLEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
IIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLPE
HALLAEGFTAEQLAAGIELYEAVGCDECTEGYKGRTGIYQVMPMTDEIGAIVLEGGNAMQIAEAAQKIGIRDLRQSALVK
AAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1134995 XTG_RS15505 WP_181901227.1 3651909..3653642(-) (pilB) [Xanthomonas euroxanthea isolate X. euroxanthea CPBF 424]
ATGAACGCAGGCTCCTTAAACCTAGTCGGTATCACTGGGATAGCAAGACGTCTGGTCCAAGACGGCGTCTTGGAAGAGGT
TGCTGCACGAGCTGCAATGGATCAGGCGTCGTCGGCTAAGGTGCCCTTGCCGCAATGGTTCGCAGAAAAAAAACTGGTCA
CTGCATCCCAGCTAGCAGCGGCCAACGCGGTCGAGTTCGGTATGCCTTTGATGGACGTGTCGGTATTCGACGCCAGCCAG
AATGCCATCAAGCTGGTCAGCGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAACCGGTTGTT
CGTGGGGGTGAGCAACCCGACCCAGACCAGGGCGCTGGACGATATCAAGTTCCACACCAACCTGGTGGTCGAGCCGATCC
TGGTCGATGAGGACCAGATCCGTCGTACCCTGGAGCAATGGCAAGCCAGCAATGCGGCGCTTGGCTCGTCGCTCGGTGAC
GACGATGAGGGGATGGGCGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTTGATGC
CAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGGTGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACATCC
ATTTCGAGCCCTATGAAGACGACTACCGGGTGCGGCTGCGGATCGACGGTTTGCTGAAGAACGTGGCCAAGGCACCGGTA
AAGCTGAACCAGCGCATCGCGGCGCGTCTGAAGGTGATGTCGCAGCTGGATATCGCCGAAAAGCGGGTGCCGCAGGACGG
GCGCATCAAGCTCAACCTGTCCAAGACCAAGCAGATCGACTTCCGCGTGAGCACCTTGCCGACCCTGTTCGGCGAAAAGG
TGGTGCTGCGTATCCTGGACGGCAGCGCGGCCAAGCTGGGCATCGAGAAGCTGGGCTACGAGGCGGACCAGCAGAAGCTG
TTCCTGGAGGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGCTGTA
TACGGCGTTGGGCATCCTCAACGACGAGACGCGCAACATTTCCACCGCCGAGGATCCGGTCGAAATCCGCCTGCCTGGCG
TCAACCAGGTGCAGCAGAACAACAAGCGCGGCATGACGTTCGCGGCGGCGTTGCGCTCGTTTCTGCGCCAGGATCCGGAC
ATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATCGCGATCAAGGCCGCGCAGACCGGTCACATGGTGCT
GTCCACGCTGCACACCAATGATGCGCCGCAGACCATCGCGCGTTTGATGAACATGGGTATTGCGCCCTACAACATCACCT
CATCGGTGACGTTGGTGATTGCGCAGCGTCTGGCGCGGCGCCTGTGCAACAACTGCAAGCGCAAATCGACCTTGCCCGAG
CATGCGTTGCTGGCGGAAGGGTTCACTGCAGAGCAGCTCGCTGCCGGTATCGAACTGTATGAGGCGGTCGGTTGCGACGA
GTGCACCGAAGGCTACAAGGGCCGTACCGGTATCTACCAGGTGATGCCGATGACCGACGAGATTGGCGCGATCGTGCTGG
AAGGCGGCAACGCGATGCAGATCGCCGAGGCGGCGCAGAAGATCGGCATCCGCGACCTGCGCCAGTCGGCGCTGGTCAAG
GCCGCGCATGGGGTGACCAGTCTGGCCGAGATCAATCGCGTAACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.338

98.44

0.555
  pilB Acinetobacter baylyi ADP1

55.458

98.44

0.546
  pilB Legionella pneumophila strain ERS1305867

51.494

98.614

0.508
  pilB Vibrio cholerae strain A1552

48.7

100

0.487
  pilF Neisseria gonorrhoeae MS11

48.768

98.44

0.48
  pilB Vibrio parahaemolyticus RIMD 2210633

46.29

98.094

0.454
  pilB Vibrio campbellii strain DS40M4

45.343

98.614

0.447

Multiple sequence alignment