Detailed information NTG1133466

Bioinformatically predicted

Overview

• Name: pilT
• Type: Machinery gene
• Locus tag: JMW01_RS08670
• Genome accession: NZ_LR890270
• Coordinates: 1847462..1848442 (-)
• Length: 326 a.a.
• NCBI ID: WP_001309723.1
• Uniprot ID: A0A148HZL8
• Organism: Escherichia coli strain MINF_7C isolate MINF_7C
• Function: type IV pilus retraction (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1842462..1853442

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
JMW01_RS08640	yggI	1843186..1843683 (+)	498	WP_000858396.1	SprT family zinc-dependent metalloprotease	-
JMW01_RS08645	endA	1843778..1844485 (+)	708	WP_000286500.1	deoxyribonuclease I	-
JMW01_RS08650	rsmE	1844565..1845296 (+)	732	WP_001222509.1	16S rRNA (uracil(1498)-N(3))-methyltransferase	-
JMW01_RS08655	gshB	1845309..1846259 (+)	951	WP_000593273.1	glutathione synthase	-
JMW01_RS08660	yqgE	1846368..1846931 (+)	564	WP_001053178.1	YqgE/AlgH family protein	-
JMW01_RS08665	ruvX	1846931..1847347 (+)	417	WP_000017111.1	Holliday junction resolvase RuvX	-
JMW01_RS08670	pilT	1847462..1848442 (-)	981	WP_001309723.1	type IV pilus twitching motility protein PilT	Machinery gene
JMW01_RS08675	yggS	1848460..1849164 (+)	705	WP_000997806.1	pyridoxal phosphate homeostasis protein	-
JMW01_RS08680	yggT	1849182..1849748 (+)	567	WP_001094831.1	osmotic shock tolerance protein YggT	-
JMW01_RS08685	yggU	1849745..1850035 (+)	291	WP_001277222.1	DUF167 family protein YggU	-
JMW01_RS08690	rdgB	1850043..1850636 (+)	594	WP_001174736.1	XTP/dITP diphosphatase	-
JMW01_RS08695	hemW	1850629..1851765 (+)	1137	WP_000239946.1	radical SAM family heme chaperone HemW	-
JMW01_RS08700	-	1852078..1853064 (+)	987	WP_000784004.1	TRAP transporter substrate-binding protein	-

Sequence

Protein

• Length: 326 a.a.
• Molecular weight: 35940.15 Da
• Isoelectric Point: 5.7980

>NTDB_id=1133466 JMW01_RS08670 WP_001309723.1 1847462..1848442(-) (pilT) [Escherichia coli strain MINF_7C isolate MINF_7C]
MNMEEIVALSVKHNVSDLHLCSAWPARWRIRGRMEAAPFDAPDVEELLREWLDDDQRAILLENGQLDFAVSLAENQRLRG
SAFAQRQGISLALRLLPSHCPQLEQLGAPPVLPELLKSENGVILVTGATGSGKSTTLAAMVGYLNQHADAHILTLEDPVE
YLYASQRCLIQQREIGLHCMTFASGLRAALREDPDVILLGELRDSETIRLALTAAETGHLVLATLHTRGAAQAVERLVDS
FPAQEKDPVRNQLAGSLRAVLSQKLEVDKQEGRVALFELLINIPAVGNLIREGKTHQLPHVIQTGQQVGMLTFQQSYQQR
VGEGRL

Nucleotide

• Length: 981 bp

>NTDB_id=1133466 JMW01_RS08670 WP_001309723.1 1847462..1848442(-) (pilT) [Escherichia coli strain MINF_7C isolate MINF_7C]
ATGAATATGGAAGAAATTGTGGCCCTTAGTGTAAAGCATAACGTCTCGGATCTACACCTGTGCAGCGCCTGGCCCGCACG
ATGGCGTATTCGCGGGCGAATGGAAGCTGCGCCGTTTGATGCGCCGGACGTCGAAGAGCTACTGCGGGAGTGGCTGGATG
ACGATCAGCGGGCAATATTGCTGGAGAATGGTCAGCTGGATTTTGCTGTGTCGCTGGCGGAAAACCAGCGGTTGCGTGGC
AGTGCGTTCGCGCAACGGCAAGGTATTTCTCTGGCATTACGGTTGTTACCTTCGCACTGTCCACAGCTCGAACAGCTTGG
TGCGCCACCGGTATTGCCGGAATTACTCAAGAGCGAGAATGGCGTGATTCTGGTGACGGGGGCGACGGGGAGCGGCAAAT
CTACCACGCTGGCGGCGATGGTTGGCTATCTCAATCAACATGCCGATGCGCATATTCTGACGCTGGAAGATCCTGTCGAA
TATCTCTATGCCAGCCAGCGATGTTTGATCCAGCAGCGGGAAATTGGTTTGCACTGTATGACGTTCGCATCGGGATTGCG
GGCTGCGTTGCGGGAAGATCCTGATGTGATTTTGCTCGGAGAGCTGCGTGACAGCGAGACAATCCGTCTGGCGCTGACGG
CGGCAGAAACCGGGCATCTGGTGCTGGCGACATTACACACGCGCGGCGCAGCGCAGGCAGTTGAGCGACTGGTGGATTCA
TTTCCGGCGCAGGAAAAAGATCCCGTGCGTAATCAACTGGCAGGGAGTTTACGGGCGGTGTTGTCACAAAAGCTGGAAGT
GGATAAACAGGAAGGACGCGTGGCGCTGTTTGAATTGCTGATTAACATTCCCGCGGTGGGGAATTTGATTCGCGAAGGGA
AAACCCACCAGTTGCCGCATGTTATTCAAACCGGGCAGCAGGTGGGGATGTTAACGTTTCAGCAGAGTTATCAGCAGCGG
GTAGGGGAAGGACGTTTGTGA

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (62-239)

3D structure

• AlphaFold DB: A0A148HZL8

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilT	Vibrio cholerae strain A1552	48.93	100	0.491
pilT	Vibrio cholerae O1 biovar El Tor strain E7946	48.93	100	0.491
pilT	Neisseria meningitidis 8013	48.78	100	0.491
pilT	Neisseria gonorrhoeae MS11	48.476	100	0.488
pilT	Acinetobacter baumannii D1279779	46.789	100	0.469
pilT	Acinetobacter baylyi ADP1	46.789	100	0.469
pilT	Acinetobacter baumannii strain A118	46.789	100	0.469
pilT	Acinetobacter nosocomialis M2	46.789	100	0.469
pilT	Pseudomonas stutzeri DSM 10701	45.872	100	0.46
pilT	Pseudomonas aeruginosa PAK	45.566	100	0.457
pilT	Legionella pneumophila strain Lp02	44.648	100	0.448
pilT	Legionella pneumophila strain ERS1305867	44.648	100	0.448
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	41.824	97.546	0.408
pilU	Vibrio cholerae strain A1552	39.21	100	0.396
pilU	Pseudomonas stutzeri DSM 10701	36.97	100	0.374
pilU	Acinetobacter baylyi ADP1	36.646	98.773	0.362