Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   WDLP6_RS05850 Genome accession   NZ_LR594671
Coordinates   1208368..1210101 (-) Length   577 a.a.
NCBI ID   WP_162591591.1    Uniprot ID   A0A6P2EAW6
Organism   Variovorax sp. PBL-E5     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1203368..1215101
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  WDLP6_RS05830 (E5CHR_01164) - 1204079..1205575 (-) 1497 WP_162591588.1 MBOAT family protein -
  WDLP6_RS05835 (E5CHR_01165) coaE 1205657..1206256 (-) 600 WP_162591589.1 dephospho-CoA kinase -
  WDLP6_RS05840 (E5CHR_01166) pilD 1206270..1207124 (-) 855 WP_162591590.1 A24 family peptidase Machinery gene
  WDLP6_RS05845 (E5CHR_01167) pilC 1207124..1208347 (-) 1224 WP_162566227.1 type II secretion system F family protein Machinery gene
  WDLP6_RS05850 (E5CHR_01168) pilB 1208368..1210101 (-) 1734 WP_162591591.1 type IV-A pilus assembly ATPase PilB Machinery gene
  WDLP6_RS05860 (E5CHR_01170) - 1210333..1211325 (-) 993 WP_162566229.1 polyprenyl synthetase family protein -
  WDLP6_RS05865 (E5CHR_01171) rplU 1211512..1211823 (+) 312 WP_162566230.1 50S ribosomal protein L21 -
  WDLP6_RS05870 (E5CHR_01172) rpmA 1211836..1212093 (+) 258 WP_162566231.1 50S ribosomal protein L27 -
  WDLP6_RS05875 (E5CHR_01173) obgE 1212162..1213217 (+) 1056 WP_162566232.1 GTPase ObgE -
  WDLP6_RS05880 (E5CHR_01174) proB 1213219..1214370 (+) 1152 WP_162591592.1 glutamate 5-kinase -
  WDLP6_RS05885 (E5CHR_01175) - 1214375..1215022 (-) 648 WP_332105448.1 CNP1-like family protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62456.76 Da        Isoelectric Point: 5.6281

>NTDB_id=1128225 WDLP6_RS05850 WP_162591591.1 1208368..1210101(-) (pilB) [Variovorax sp. PBL-E5]
MAAAEPLVKESSPIALPGLARALISAGKLPAKVAEDIYQKSLSGRSSFIAELTGTGAVSAADLAHTLSSAFGAPLLDLDA
IDPQRLPKDLLDPKLCTAYRVVVLSKRNNRLIVATADPSDQQAAEKIKFASQMGVDWVIAEYDKLSRMIEAAAVSAAETI
DNIIGAEFEFDEVSSDTSSEANDSAAAEVEDAPVVRFLHKMLLDAFSMRASDIHFEPYEHQYRVRFRVDGELREIASPPT
VIKDKLASRIKVISRLDISEKRIPQDGRMKLKIGPERVIDFRVSTLPTLFGEKIVIRILDPSSARLGIDALGYDADEKER
LLTAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGVNIATAEDPSEINLPGVNQVNVNEKAGLTFATALRAFLRQDPD
IIMVGEIRDLETADISIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCTVCRVPADVPA
QALLDAGFKAQDVDNSWKPYRPVGCSACNSGYKGRVGIYQVMPITEEIQKIILRDGSALDIAKQAEAEGVRSLRQSGLKK
VMQGLTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=1128225 WDLP6_RS05850 WP_162591591.1 1208368..1210101(-) (pilB) [Variovorax sp. PBL-E5]
ATGGCCGCTGCCGAACCCCTTGTCAAAGAAAGCTCGCCGATCGCCCTTCCAGGGCTCGCGCGTGCGTTGATCTCGGCCGG
AAAGCTGCCGGCCAAGGTCGCCGAGGACATCTATCAGAAGTCGCTCAGCGGCCGCAGCAGCTTCATTGCTGAGCTCACAG
GCACCGGCGCCGTGTCGGCCGCCGACCTGGCTCACACGCTGTCCAGCGCCTTCGGCGCCCCATTGCTGGACCTGGACGCC
ATCGACCCGCAGCGGCTGCCGAAGGACCTTCTCGACCCCAAGCTTTGCACGGCCTACCGGGTGGTCGTCCTCAGCAAGCG
CAACAACCGTCTCATTGTCGCAACAGCTGACCCTTCCGATCAGCAGGCCGCCGAAAAGATCAAGTTTGCGTCGCAGATGG
GCGTGGACTGGGTCATTGCCGAGTACGACAAGCTCTCGCGCATGATCGAAGCGGCCGCCGTGAGCGCGGCCGAAACCATC
GACAACATCATCGGCGCCGAGTTCGAGTTCGACGAGGTGTCCTCGGACACTTCGAGCGAGGCCAACGATTCCGCGGCGGC
CGAAGTCGAGGATGCGCCGGTCGTGCGCTTCCTGCACAAGATGCTGCTCGATGCCTTCAGCATGCGCGCGTCGGACATCC
ACTTCGAGCCCTACGAACATCAATACCGCGTGCGCTTTCGCGTCGATGGCGAATTGCGCGAGATCGCAAGCCCGCCCACG
GTCATCAAGGACAAGCTGGCCTCCCGCATCAAGGTGATTTCGCGCCTGGATATTTCCGAGAAGCGGATTCCGCAGGACGG
CCGGATGAAGCTCAAGATCGGTCCGGAGCGTGTGATCGATTTTCGCGTGAGCACGCTGCCCACGTTGTTCGGCGAAAAGA
TCGTCATCCGTATCCTCGACCCGAGTAGCGCACGCCTGGGCATCGATGCGCTGGGCTACGACGCGGACGAAAAGGAGCGC
CTGCTCACCGCGATCGGACGTCCTTACGGCATGGTGCTGGTCACCGGGCCCACGGGTTCCGGCAAGACGGTGTCGCTCTA
CACCTGCCTGAACCTGCTCAACAAGCCCGGTGTCAACATCGCCACGGCCGAAGACCCGTCCGAAATCAACCTTCCCGGCG
TGAACCAGGTCAACGTCAACGAAAAGGCGGGACTGACCTTCGCCACCGCGCTGCGCGCGTTCCTGCGGCAGGATCCCGAC
ATCATCATGGTCGGCGAAATCCGCGACCTCGAAACGGCCGACATCTCGATCAAGGCCGCGCAGACGGGCCACCTGGTTCT
CTCCACGCTGCACACCAACGACGCTCCGACGACGCTGACGCGGATGCGCAACATGGGTATCGCGCCCTTCAACATCGCGT
CGAGCGTGATCCTGATCACCGCGCAGCGGCTCGCACGCCGCCTGTGCACGGTCTGCCGCGTGCCGGCGGACGTGCCCGCG
CAGGCCTTGCTCGATGCCGGCTTCAAGGCGCAGGACGTGGACAACTCCTGGAAGCCCTACCGCCCCGTCGGCTGCTCGGC
CTGCAACAGCGGCTACAAGGGACGTGTCGGCATCTACCAGGTGATGCCGATCACGGAAGAAATCCAGAAGATCATCCTGC
GCGACGGCAGCGCCCTCGACATCGCCAAGCAGGCCGAGGCCGAGGGCGTTCGCTCGCTGCGGCAATCTGGACTGAAGAAG
GTCATGCAAGGGCTGACTTCACTTGAAGAAGTGCTGGCAGTCACCAACGAGTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(55-153)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6P2EAW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

54.225

98.44

0.534
  pilB Acinetobacter baumannii D1279779

54.367

97.227

0.529
  pilF Neisseria gonorrhoeae MS11

52.753

97.574

0.515
  pilB Legionella pneumophila strain ERS1305867

50

98.094

0.49
  pilB Vibrio campbellii strain DS40M4

45.439

98.787

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

48.099

91.161

0.438
  pilB Vibrio cholerae strain A1552

48.738

89.255

0.435
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.585

94.801

0.385
  pilF Thermus thermophilus HB27

38.144

100

0.385

Multiple sequence alignment