Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   EL295_RS29830 Genome accession   NZ_LR134308
Coordinates   6122141..6124285 (+) Length   714 a.a.
NCBI ID   WP_003146401.1    Uniprot ID   A0A9P1VWE9
Organism   Pseudomonas aeruginosa strain NCTC11445     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6117141..6129285
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  EL295_RS29810 (NCTC11445_05839) pilM 6119286..6120350 (+) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  EL295_RS29815 (NCTC11445_05840) pilN 6120350..6120946 (+) 597 WP_031671448.1 type 4a pilus biogenesis protein PilN -
  EL295_RS29820 (NCTC11445_05841) pilO 6120943..6121566 (+) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO -
  EL295_RS29825 (NCTC11445_05842) pilP 6121563..6122087 (+) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP -
  EL295_RS29830 (NCTC11445_05843) pilQ 6122141..6124285 (+) 2145 WP_003146401.1 type 4a pilus secretin PilQ Machinery gene
  EL295_RS29835 (NCTC11445_05844) aroK 6124297..6124815 (+) 519 WP_003095833.1 shikimate kinase AroK -
  EL295_RS29840 (NCTC11445_05845) aroB 6124865..6125971 (+) 1107 WP_126453420.1 3-dehydroquinate synthase -
  EL295_RS29845 (NCTC11445_05846) - 6125978..6127627 (+) 1650 WP_033973350.1 AAA family ATPase -

Sequence

Protein


Download         Length: 714 a.a.        Molecular weight: 77359.88 Da        Isoelectric Point: 5.2219

>NTDB_id=1120954 EL295_RS29830 WP_003146401.1 6122141..6124285(+) (pilQ) [Pseudomonas aeruginosa strain NCTC11445]
MNSGLSRLGIALLAAMFAPALLAADLEKLDVAALPGDRVELKLQFDEPVAAPRGYTIEQPARIALDLPGVQNKLGTKNRE
LSVGNTRSVTVVEAKDRTRLIINLTTLSSYTTRVEGNNLFVVVGNSPAGASVASTAPVKASPAPASYAQPIKPKPYVPAG
RAIRNIDFQRGEKGEGNVVIDLSDPTLSPDIQEQGGKIRLDFAKTQLPDALRVRLDVKDFATPVQFVNASAQSDRTSITI
EPSGLYDYLVYQTDNRLTVSIKPMTTEDAERRKKDNFAYTGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITL
RLQNVPWDQALDLVLKTKGLDKRKLGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKAADIAKLFQSVT
SDGGQEGKEGGRGSITVDDRTNSIIAYQPQERLDELRRIVSQLDIPVRQVMIEARIVEANVGYDKSLGVRWGGAYHKGNW
NGYGKDGNIGIKDEDGMNCGPIAGNCTFPTTGTSKSPSPFVDIGAKDATSGIGIGFITDNIILDLQLSAMEKTGNGEIVS
QPKVVTSDKETAKILKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIVEVKVTKDAPDFDRALNGVPPINKN
EVNAKILVNDGETIVIGGVFSNTQSKSVDKVPFLGDLPYLGRLFRRDTVSDVKNELLVFLTPRIMNNQAIAIGR

Nucleotide


Download         Length: 2145 bp        

>NTDB_id=1120954 EL295_RS29830 WP_003146401.1 6122141..6124285(+) (pilQ) [Pseudomonas aeruginosa strain NCTC11445]
ATGAACAGTGGCCTCTCGCGCCTCGGGATCGCTTTGCTGGCCGCCATGTTCGCGCCGGCATTGCTCGCCGCGGACCTGGA
GAAACTCGACGTGGCGGCATTGCCTGGCGACCGGGTCGAGCTAAAGCTGCAGTTCGACGAGCCCGTCGCCGCGCCGCGTG
GCTATACCATCGAGCAGCCGGCGCGAATCGCCCTCGATCTGCCGGGGGTACAGAACAAGCTCGGCACCAAGAACCGCGAG
CTGAGCGTGGGCAATACGCGCAGCGTCACGGTGGTCGAGGCGAAGGATCGCACACGGTTGATCATCAACCTGACCACGTT
GTCGTCCTACACCACCCGGGTGGAAGGCAACAACCTCTTCGTGGTGGTCGGCAATTCGCCGGCCGGCGCCAGCGTCGCTT
CCACCGCTCCGGTCAAGGCTAGCCCGGCTCCGGCCAGCTATGCCCAGCCGATCAAGCCCAAGCCGTACGTGCCGGCGGGT
CGTGCCATTCGCAACATCGATTTCCAGCGTGGCGAAAAGGGCGAGGGCAACGTGGTCATCGACCTGTCCGACCCGACCTT
GAGTCCGGATATCCAGGAGCAGGGCGGCAAGATCCGCCTGGACTTCGCCAAGACCCAGCTGCCGGATGCGCTGCGGGTTC
GCCTGGACGTCAAGGACTTCGCCACGCCGGTGCAGTTCGTCAACGCCAGCGCGCAATCCGACCGTACCAGCATCACCATC
GAGCCGAGCGGGCTCTACGACTATCTGGTCTACCAGACCGACAATCGCCTGACCGTCAGCATCAAGCCGATGACCACGGA
AGACGCCGAGCGGCGCAAGAAGGACAATTTCGCCTATACCGGTGAGAAACTGTCGCTGAACTTCCAGGACATCGACGTGC
GTTCGGTACTGCAGCTGATCGCCGACTTCACCGACCTGAACCTGGTGGCCTCGGATACCGTGCAGGGCAATATCACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAGACCAAGGGCCTGGACAAGCGCAAGCTCGGCAA
CGTGCTCCTGGTGGCCCCGGCCGACGAGATCGCCGCCCGTGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATCGCCGAGT
TGGCGCCACTGCGTCGGGAACTGATCCAGGTGAACTACGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGATGGTGGCCAGGAAGGCAAGGAAGGCGGGCGCGGCTCGATCACCGTCGACGACCGCACCAACAGCATCATCGCCTA
TCAGCCCCAGGAGCGACTGGACGAACTGCGGCGGATCGTTTCGCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAGG
CACGCATCGTCGAGGCCAACGTCGGTTACGACAAGAGCCTCGGTGTGCGCTGGGGCGGCGCTTATCACAAGGGCAACTGG
AACGGCTACGGCAAGGACGGCAATATCGGGATCAAGGACGAGGACGGCATGAACTGCGGCCCGATCGCCGGCAACTGTAC
TTTCCCCACCACGGGAACCAGCAAGTCGCCGTCGCCGTTCGTCGACATCGGCGCCAAGGATGCCACCTCCGGGATCGGCA
TCGGCTTCATCACCGACAACATCATCCTCGACCTGCAACTGTCGGCGATGGAAAAGACCGGCAACGGCGAGATCGTCTCC
CAGCCCAAGGTCGTGACCTCGGACAAGGAAACCGCGAAGATCCTCAAGGGCCAGGAAGTACCCTACCAGGAGGCCAGCTC
CAGTGGCGCTACCAGCACCTCGTTCAAGGAGGCCGCGCTGTCCCTCGAGGTGACCCCGCAGATCACCCCGGACAACCGGA
TCATCGTCGAGGTGAAGGTGACCAAGGATGCGCCGGACTTCGATCGCGCACTGAACGGCGTACCGCCGATCAACAAGAAC
GAGGTCAACGCCAAGATCCTGGTCAACGACGGCGAGACCATCGTCATCGGCGGCGTGTTCAGCAACACCCAGAGCAAGTC
GGTGGACAAAGTGCCGTTCCTCGGCGACCTGCCTTACCTGGGGCGGCTGTTCCGGCGCGATACGGTCTCGGACGTGAAAA
ACGAACTTCTGGTTTTCCTGACACCGCGGATCATGAATAATCAGGCCATCGCAATCGGTCGCTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(381-449)

Secretin and TonB N terminus short domain (STN)

(307-353)

AMIN domain (AMIN)

(38-122)

Bacterial type II and III secretion system protein (Secretin)

(549-705)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

98.179

100

0.982
  pilQ Acinetobacter baumannii D1279779

41.268

99.44

0.41
  pilQ Acinetobacter baumannii strain A118

41.268

99.44

0.41
  comQ Acinetobacter baylyi ADP1

41.738

98.319

0.41

Multiple sequence alignment