Detailed information    

insolico Bioinformatically predicted

Overview

Name   pepF   Type   Regulator
Locus tag   ACK25W_RS05470 Genome accession   NZ_CP179722
Coordinates   1120025..1121851 (+) Length   608 a.a.
NCBI ID   WP_282959334.1    Uniprot ID   -
Organism   Bacillus pumilus strain FJAT-56819     
Function   degradation of XIP; competence shut-off (predicted from homology)   
Competence regulation

Genomic Context

Location: 1115025..1126851
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACK25W_RS05445 (ACK25W_05445) - 1115289..1115954 (-) 666 WP_060596006.1 TerC family protein -
  ACK25W_RS05450 (ACK25W_05450) - 1116130..1116250 (+) 121 Protein_1016 hypothetical protein -
  ACK25W_RS05455 (ACK25W_05455) mecA 1116217..1116882 (+) 666 WP_012009573.1 adaptor protein MecA Regulator
  ACK25W_RS05460 (ACK25W_05460) cls 1116968..1118488 (+) 1521 WP_106071567.1 cardiolipin synthase -
  ACK25W_RS05465 (ACK25W_05465) - 1118650..1119810 (+) 1161 WP_060596008.1 competence protein CoiA -
  ACK25W_RS05470 (ACK25W_05470) pepF 1120025..1121851 (+) 1827 WP_282959334.1 oligoendopeptidase F Regulator
  ACK25W_RS05475 (ACK25W_05475) - 1121955..1122128 (-) 174 WP_034663238.1 hypothetical protein -
  ACK25W_RS05480 (ACK25W_05480) - 1122425..1123336 (-) 912 WP_034663234.1 ClpXP adapter SpxH family protein -
  ACK25W_RS05485 (ACK25W_05485) - 1123333..1123731 (-) 399 WP_008355333.1 thiol management oxidoreductase -
  ACK25W_RS05490 (ACK25W_05490) - 1124007..1124774 (-) 768 WP_034663232.1 lytic transglycosylase domain-containing protein -
  ACK25W_RS05495 (ACK25W_05495) - 1124796..1125374 (-) 579 WP_414051804.1 CYTH domain-containing protein -
  ACK25W_RS05500 (ACK25W_05500) - 1125611..1125976 (+) 366 WP_414051805.1 hypothetical protein -
  ACK25W_RS05505 (ACK25W_05505) - 1126010..1126639 (+) 630 WP_003211990.1 GTP pyrophosphokinase family protein -

Sequence

Protein


Download         Length: 608 a.a.        Molecular weight: 70160.88 Da        Isoelectric Point: 5.4474

>NTDB_id=1092841 ACK25W_RS05470 WP_282959334.1 1120025..1121851(+) (pepF) [Bacillus pumilus strain FJAT-56819]
MTSQSKNNHLPDRSEVKEENKWRLEDIFESVDAWNKEFEAVKKEIPKLAQFKGKLAHSADVLYEALTFQDQLSEKLGKLY
TYAHMKYDEDTTNSSFQALNDKASNLFTQLSSTSAYIVPEILSIQEDKLQQFILEKEELKLYSHALEEINKERPHILSEE
QEALLAEASEPLSTSSTTFSMFNNADISFPSVKDENGEEKKITHGNFITFLNSDDREVRKNAFKAVYKTYDQYKNTLAST
LSGSIKKDNFYAKVRNYNSAREAALSRNSIPEEVYDNLIDTVHQYLPLLHRYIELRKKVLKLDEVHNYDLYTPLVKDAGM
KLTYDEAKDYMLKGLAPLGEEYVSVLKEGLSNRWVDVYENKGKRSGAYSSGSYGTNPYILMNWQNNIDNLFTLAHEFGHS
VHSYYTRKHQPYPYGNYSIFVAEVASTTNEALLGEYLLNHLDDKKQRLYVLNHLLEGFRGTVFRQTMFAEFEHLIHVKAQ
EGEALTPEFMTNLYYDLNKKYFGDGMVVDKEIGLEWTRIPHFYYNYYVYQYATGYSAAQALSKQILEEGKPAVERYTDFL
KAGSSDYPINVLKKAGVDMASKEPIKAACQLFEEKLKEMEELISKTGE

Nucleotide


Download         Length: 1827 bp        

>NTDB_id=1092841 ACK25W_RS05470 WP_282959334.1 1120025..1121851(+) (pepF) [Bacillus pumilus strain FJAT-56819]
ATGACTTCACAAAGCAAAAATAACCATTTACCAGACAGAAGCGAAGTAAAAGAAGAAAACAAATGGCGTCTTGAAGATAT
TTTCGAAAGCGTTGATGCATGGAACAAGGAATTCGAAGCTGTCAAAAAAGAAATTCCTAAGCTAGCCCAATTTAAAGGCA
AGCTGGCCCATTCTGCTGATGTACTGTATGAAGCCCTAACCTTTCAGGATCAGCTTTCTGAAAAGCTTGGTAAGCTTTAT
ACATATGCGCACATGAAATACGATGAGGATACAACTAACTCATCATTTCAAGCGCTGAATGATAAAGCATCGAATTTATT
TACACAGCTCTCTAGTACTTCTGCTTATATTGTTCCAGAGATTTTATCGATTCAAGAAGATAAATTGCAGCAGTTTATTC
TGGAAAAAGAAGAATTAAAACTGTATTCCCATGCTCTCGAAGAAATTAATAAAGAGCGTCCACATATTTTAAGTGAGGAG
CAAGAAGCTTTATTAGCTGAGGCCTCTGAACCACTCTCAACTTCATCTACTACTTTTAGTATGTTTAACAATGCTGATAT
TTCGTTCCCTTCTGTAAAGGATGAAAATGGGGAAGAAAAGAAGATTACCCACGGCAACTTTATCACATTTTTAAACAGTG
ATGACCGTGAAGTGAGAAAGAATGCCTTTAAAGCTGTGTATAAAACGTATGATCAGTATAAAAACACGCTTGCTTCGACA
CTGAGCGGTTCGATTAAAAAGGATAATTTTTATGCAAAAGTACGAAATTATAACTCAGCAAGAGAAGCGGCACTATCTCG
GAACAGTATTCCAGAAGAAGTATATGACAATCTGATTGATACAGTTCATCAATATTTACCGTTATTACACCGTTATATTG
AATTGCGTAAAAAGGTATTAAAGCTTGATGAAGTGCACAATTATGACTTGTATACACCACTTGTAAAAGATGCTGGTATG
AAGCTAACCTACGATGAAGCAAAAGATTATATGCTAAAAGGGTTAGCGCCATTAGGAGAAGAATATGTGTCCGTGTTAAA
AGAGGGACTGAGCAATCGCTGGGTAGACGTTTATGAGAATAAAGGGAAACGCAGCGGTGCGTATTCATCTGGAAGCTATG
GTACAAATCCATATATCCTCATGAACTGGCAAAATAATATTGATAATTTATTTACACTGGCTCATGAATTTGGGCACTCT
GTCCACAGCTACTATACAAGAAAGCATCAGCCATATCCGTATGGAAACTATAGTATCTTTGTAGCTGAAGTGGCTTCTAC
GACAAACGAAGCGTTACTTGGCGAATACCTGCTGAATCATCTAGATGATAAAAAGCAGCGTTTGTATGTGTTAAATCACT
TGTTAGAAGGATTCAGAGGAACGGTATTTAGACAGACGATGTTTGCTGAGTTTGAGCATCTTATTCATGTGAAAGCGCAA
GAAGGAGAGGCTTTAACGCCGGAATTTATGACGAACCTTTATTACGACCTGAATAAGAAGTATTTTGGAGACGGCATGGT
TGTGGATAAAGAAATCGGATTAGAATGGACGAGAATTCCTCACTTCTATTACAACTATTATGTATATCAATATGCAACAG
GCTATAGTGCTGCACAGGCATTAAGCAAGCAAATATTAGAAGAAGGAAAACCTGCGGTGGAGCGCTACACAGACTTCCTT
AAAGCAGGAAGCTCTGATTATCCAATCAATGTGTTGAAAAAAGCAGGTGTTGATATGGCTTCCAAAGAACCAATCAAAGC
AGCATGTCAATTGTTCGAAGAAAAGCTGAAAGAAATGGAAGAGCTCATCTCTAAAACGGGTGAGTGA

Domains

Predicted by InterproScan.

Peptidase family M3 (Peptidase_M3)

(211-590)

Oligopeptidase F (Peptidase_M3_N)

(120-189)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pepF Streptococcus salivarius strain HSISS4

49.158

97.697

0.48

Multiple sequence alignment