Detailed information    

insolico Bioinformatically predicted

Overview

Name   addB   Type   Machinery gene
Locus tag   ACL6EQ_RS04790 Genome accession   NZ_CP178716
Coordinates   940310..943810 (+) Length   1166 a.a.
NCBI ID   WP_413360331.1    Uniprot ID   -
Organism   Bacillus subtilis strain SY1483     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 935310..948810
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL6EQ_RS04770 (ACL6EQ_04770) yhjO 936169..937374 (-) 1206 WP_137200694.1 MDR family MFS transporter -
  ACL6EQ_RS04775 (ACL6EQ_04775) yhjP 937488..939215 (+) 1728 WP_305090558.1 SgrR family transcriptional regulator -
  ACL6EQ_RS04780 (ACL6EQ_04780) cusC 939245..939571 (-) 327 WP_153255879.1 four-helix bundle copper-binding protein -
  ACL6EQ_RS04785 (ACL6EQ_04785) yhjR 939689..940126 (-) 438 WP_167560008.1 ferritin family protein -
  ACL6EQ_RS04790 (ACL6EQ_04790) addB 940310..943810 (+) 3501 WP_413360331.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ACL6EQ_RS04795 (ACL6EQ_04795) addA 943797..947495 (+) 3699 WP_217019750.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ACL6EQ_RS04800 (ACL6EQ_04800) sbcD 947567..948742 (+) 1176 WP_217019749.1 exonuclease subunit SbcD -

Sequence

Protein


Download         Length: 1166 a.a.        Molecular weight: 134744.82 Da        Isoelectric Point: 5.5781

>NTDB_id=1090746 ACL6EQ_RS04790 WP_413360331.1 940310..943810(+) (addB) [Bacillus subtilis strain SY1483]
MGAEFLVGRSGSGKTKLIINSIQDELRRAPFGKPIIFLVPDQMTFLMEYELAKTPDMGGMIRAQVFSFSRLAWRVLQHTG
GMSRPFLTSTGVQMLLRRLIEEHKQEFKVYQKASDKSGFTAQVERMLTEFKRYCLEPEDIRRMAESGTASEYRGERVLSE
KLHDLSILYQQMEKSLADQYLHSEDYLTLLAEHIPLAEDIKGAHIYVDGFYQFTPQEFRVLEQLMVHAEHITFSLTADKP
SYEREPHELELFRMTGKTYYRLHQKAKELNLDITYKELSGTERHTKTPELAHLETQYEARPAVPYAEKQEALTVMQAANR
RAELEGIAREIHALVREKGYRYKDVAILVRQPEDYKDMVKEVFADYEIPYFIDGKASMLNHPLIEFIRSSLDVLKGNWRY
EAVFRCVKTELLFPLNEPKAKVREQVDQLENYCIAYGIKGDRWTKGDRFQYRRFVSLDDDFAQTDQEIEMENMLNDTRDW
IVPPLFQLQKRMKKAKTVQEKAEALYRYLEETDVPLKLDQERQRAEDDGRIIEAQQHQQAWDAVIQLLEEFVEMMGDDEI
SLDLFQQMIEAGAESLTFSLIPPALDQVFVGNMDLSRMYGTSCTFVLGANDGVLPARPDENGVLSDDDREWLKTIGVELS
SGGRERLLDEHFLIYMAFSSPSDRLYVSYPIADAEGKTLLPSMIVKRLEELFPHHKERLLTNEPEQVSDEEQLMYVVNKS
VAQSFTASQLRLWTREYDISDVWWSTYNVLMSEQDRLQSKKLFSSLFFRNEVKQLERSVSRQLYGERIQGSVSRMETFNA
CPFSHFASHGLHLKERQFFKLEAPDIGQLFHSSLKLISDRLREKKLDWRDLTKEQCELFSYDAVERLAPKLQKEILLSSN
RHYYVKEKLQKIVTRVSGILSEHAKASGFVPIGLELGFGGKGPLPPLTFQLKNGCTMELVGRIDRVDKAESSKGLLLRIV
DYKSSDKGLDLAEVYYGLALQMLTYLDLSITHSADWLGMRATPAGVLYFHIHDPMIQSNLPLGLDEIEQEIFKKFKMKGL
LLGDQEVVRLMDTTLQEGRSNIINAGLKKDGSLRSDSAAVGEKEFDLLTKHVRRTFQEAGEQITDGRVSIEPYKMKNKTP
CTYCVFKSVCQFDESLEENEYRPLKAEKDKTILEWIKKEADGNEHS

Nucleotide


Download         Length: 3501 bp        

>NTDB_id=1090746 ACL6EQ_RS04790 WP_413360331.1 940310..943810(+) (addB) [Bacillus subtilis strain SY1483]
TTGGGAGCAGAGTTTTTAGTAGGCAGGTCCGGGAGTGGAAAAACGAAGCTGATCATCAATAGCATTCAGGATGAATTGCG
CCGGGCTCCATTCGGGAAGCCGATCATTTTTCTAGTCCCGGATCAAATGACGTTTTTAATGGAATACGAGCTTGCTAAAA
CGCCAGATATGGGCGGGATGATACGCGCTCAAGTGTTCAGTTTTTCACGATTGGCCTGGCGCGTCCTCCAGCATACGGGA
GGAATGAGCAGGCCGTTTCTGACGAGCACCGGCGTACAAATGCTCCTGCGGAGGCTCATTGAGGAGCATAAACAGGAGTT
CAAAGTCTATCAAAAAGCGAGTGACAAAAGCGGGTTTACCGCACAGGTTGAGCGGATGCTGACAGAGTTTAAGCGCTATT
GTCTGGAACCGGAGGATATCCGCCGGATGGCGGAAAGCGGAACGGCTTCCGAGTATCGCGGAGAACGTGTTTTATCTGAA
AAGCTCCATGACTTATCAATTCTGTATCAGCAAATGGAAAAAAGCCTCGCAGACCAATATCTTCACTCTGAGGATTATTT
GACATTGCTGGCAGAGCATATCCCGCTTGCGGAAGATATAAAAGGCGCCCATATCTATGTGGATGGCTTTTATCAGTTTA
CACCGCAGGAATTCAGGGTGTTGGAGCAGCTTATGGTTCATGCGGAGCATATCACGTTTTCGCTCACAGCGGACAAGCCG
TCATATGAGCGGGAGCCGCATGAACTGGAATTGTTCAGAATGACGGGGAAAACCTATTACCGCCTGCATCAGAAGGCGAA
GGAACTGAATCTGGACATTACGTATAAAGAGCTGAGCGGGACTGAGCGGCATACAAAGACGCCGGAATTGGCGCATCTAG
AGACACAGTATGAAGCGCGTCCGGCCGTTCCATACGCAGAAAAACAAGAAGCCCTTACTGTGATGCAGGCCGCAAACAGA
CGAGCTGAGCTGGAAGGCATTGCCCGGGAAATTCACGCCCTAGTCAGAGAGAAGGGATATCGCTATAAGGATGTAGCCAT
TCTTGTGCGCCAGCCGGAAGACTACAAGGATATGGTGAAGGAAGTTTTCGCAGATTACGAGATTCCTTATTTCATTGACG
GAAAAGCATCTATGCTGAACCACCCGTTAATCGAATTTATCCGGTCAAGCCTTGATGTTCTAAAAGGGAATTGGCGTTAT
GAAGCGGTGTTTCGCTGCGTGAAAACCGAACTGCTATTCCCGCTCAATGAACCGAAGGCCAAAGTGAGAGAACAGGTCGA
TCAGCTCGAAAATTACTGTATCGCCTATGGTATTAAAGGCGACCGCTGGACAAAGGGCGATCGTTTCCAATACAGGCGTT
TTGTGTCATTGGATGATGATTTTGCACAGACTGATCAGGAAATCGAAATGGAAAACATGTTGAATGACACCCGCGATTGG
ATTGTTCCGCCGCTTTTTCAGCTTCAAAAACGCATGAAAAAAGCGAAGACGGTTCAAGAGAAGGCGGAGGCGCTCTATCG
TTATTTAGAAGAGACGGATGTGCCGCTGAAGCTGGATCAGGAAAGGCAGCGTGCTGAGGATGACGGCAGAATCATTGAAG
CGCAGCAGCATCAGCAGGCGTGGGACGCAGTCATTCAGCTGCTTGAGGAGTTTGTTGAAATGATGGGAGATGATGAGATT
TCTCTTGATTTGTTTCAGCAAATGATAGAAGCCGGCGCGGAGTCGCTTACATTTTCTCTAATTCCGCCTGCGCTTGACCA
GGTGTTTGTCGGCAATATGGATTTGTCCAGAATGTATGGCACCTCCTGCACCTTTGTGCTCGGGGCAAACGACGGCGTTC
TGCCGGCACGCCCTGATGAAAACGGGGTCCTGTCGGATGATGACCGGGAATGGCTGAAAACGATTGGGGTTGAGCTATCC
TCAGGCGGACGGGAGCGTTTGCTTGATGAACATTTCCTCATCTACATGGCGTTTTCAAGTCCGTCTGACCGGCTTTACGT
ATCGTATCCGATTGCTGATGCAGAAGGAAAAACGCTTTTGCCGTCGATGATCGTTAAGCGGCTGGAAGAACTGTTTCCGC
ATCATAAGGAGCGCCTGTTAACAAATGAACCTGAACAGGTCAGCGATGAGGAACAGCTGATGTATGTCGTGAATAAAAGC
GTGGCACAGTCCTTTACCGCGAGCCAGCTCAGGTTATGGACTCGGGAATACGACATTAGCGACGTCTGGTGGAGCACGTA
CAATGTGCTGATGAGTGAGCAGGACAGGCTGCAATCGAAAAAGCTGTTCTCAAGCCTGTTTTTCCGGAATGAAGTGAAGC
AGCTTGAACGCAGCGTGTCGAGACAGCTCTATGGTGAACGCATTCAGGGCAGTGTCTCAAGAATGGAAACCTTTAACGCA
TGCCCGTTTTCCCATTTTGCGTCACACGGGCTGCATCTGAAGGAACGGCAATTCTTCAAGCTTGAAGCACCGGATATCGG
CCAGCTGTTTCATTCCAGCTTAAAGCTGATTTCAGACAGGCTGCGTGAGAAAAAGCTGGATTGGCGCGATTTAACGAAAG
AGCAATGCGAGCTGTTTTCCTATGATGCGGTAGAGCGGCTGGCACCGAAACTGCAAAAGGAAATTCTGCTCAGCTCAAAC
CGGCATTATTATGTGAAGGAAAAACTGCAAAAAATTGTGACACGTGTATCCGGCATTTTAAGCGAGCATGCGAAAGCGAG
CGGATTCGTACCGATCGGGCTTGAACTGGGCTTTGGAGGAAAAGGGCCGCTTCCGCCGCTGACCTTTCAACTGAAAAACG
GCTGTACGATGGAACTCGTCGGGCGAATTGACCGTGTTGATAAGGCTGAAAGCTCAAAAGGCCTGCTCCTCAGGATTGTC
GATTATAAATCAAGCGACAAAGGCCTTGACTTAGCGGAAGTATATTACGGATTGGCACTGCAAATGCTGACGTACCTTGA
TTTATCGATTACACATTCAGCTGACTGGCTCGGGATGAGGGCGACGCCTGCCGGAGTGCTGTATTTCCATATTCATGACC
CGATGATTCAATCTAATCTCCCGCTTGGGCTTGACGAGATTGAACAGGAGATCTTTAAGAAATTTAAAATGAAGGGCTTG
CTCCTCGGTGATCAGGAAGTGGTTCGCCTCATGGATACAACCCTTCAAGAGGGACGTTCAAATATCATAAACGCCGGCTT
GAAAAAAGACGGATCTCTTAGATCAGACTCGGCCGCAGTCGGTGAAAAGGAATTTGATCTTTTGACAAAGCATGTGCGCC
GCACCTTCCAGGAAGCGGGCGAACAAATCACCGACGGACGCGTATCCATTGAGCCGTACAAAATGAAGAACAAGACGCCG
TGCACATACTGTGTGTTCAAATCAGTATGCCAATTTGATGAATCACTGGAAGAAAACGAGTATCGCCCATTAAAGGCTGA
AAAGGACAAGACGATACTTGAGTGGATAAAAAAGGAGGCGGATGGCAATGAACATTCCTAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(790-1131)

UvrD-like helicase C-terminal domain (UvrD_C)

(305-668)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addB Bacillus subtilis subsp. subtilis str. 168

99.485

100

0.995

Multiple sequence alignment