Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   ACL66B_RS18380 Genome accession   NZ_CP178617
Coordinates   3528588..3529979 (-) Length   463 a.a.
NCBI ID   WP_041850475.1    Uniprot ID   -
Organism   Bacillus subtilis strain QXKL-3     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3523588..3534979
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACL66B_RS18345 (ACL66B_18345) flgL 3523759..3524655 (-) 897 WP_015714797.1 flagellar hook-associated protein FlgL -
  ACL66B_RS18350 (ACL66B_18350) flgK 3524666..3526189 (-) 1524 WP_003228001.1 flagellar hook-associated protein FlgK -
  ACL66B_RS18355 (ACL66B_18355) flgN 3526208..3526690 (-) 483 WP_014481048.1 flagellar protein FlgN -
  ACL66B_RS18360 (ACL66B_18360) flgM 3526706..3526972 (-) 267 WP_014481049.1 flagellar biosynthesis anti-sigma factor FlgM -
  ACL66B_RS18365 (ACL66B_18365) yvyF 3527053..3527472 (-) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  ACL66B_RS18370 (ACL66B_18370) comFC 3527546..3528268 (-) 723 WP_014481051.1 comF operon protein ComFC Machinery gene
  ACL66B_RS18375 (ACL66B_18375) comFB 3528232..3528528 (-) 297 WP_003227989.1 late competence protein ComFB -
  ACL66B_RS18380 (ACL66B_18380) comFA 3528588..3529979 (-) 1392 WP_041850475.1 ATP-dependent helicase ComFA Machinery gene
  ACL66B_RS18385 (ACL66B_18385) fakBA 3530085..3530930 (-) 846 WP_003227986.1 DegV family protein -
  ACL66B_RS18390 (ACL66B_18390) degU 3531028..3531717 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  ACL66B_RS18395 (ACL66B_18395) degS 3531800..3532957 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  ACL66B_RS18400 (ACL66B_18400) yvyE 3533174..3533827 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52485.50 Da        Isoelectric Point: 9.9185

>NTDB_id=1090590 ACL66B_RS18380 WP_041850475.1 3528588..3529979(-) (comFA) [Bacillus subtilis strain QXKL-3]
MNVPVEKNSSFSRELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRGYRCNRCGQTDQRYYSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWEEENAPNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTIVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIFFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=1090590 ACL66B_RS18380 WP_041850475.1 3528588..3529979(-) (comFA) [Bacillus subtilis strain QXKL-3]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCTAGAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGAAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGGGATATAGATGTAATAGGTGCGGCCAAACTGATCAGCGGTATTATTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGGAAGAGGAAAATGC
ACCAAACTGGAAGTCAATTAAACTGACATGGGACGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGGCT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCATCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATCTTTACGGAAAGTGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTTCTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

97.84

100

0.978

Multiple sequence alignment