Detailed information    

insolico Bioinformatically predicted

Overview

Name   addA   Type   Machinery gene
Locus tag   ABFT42_RS05825 Genome accession   NZ_CP178596
Coordinates   1169972..1173676 (+) Length   1234 a.a.
NCBI ID   WP_413287123.1    Uniprot ID   -
Organism   Bacillus mojavensis strain C3     
Function   homologous recombination; plasmid transformation (predicted from homology)   
Homologous recombination

Genomic Context

Location: 1164972..1178676
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABFT42_RS05810 (ABFT42_05810) - 1165449..1165763 (-) 315 WP_229147768.1 four-helix bundle copper-binding protein -
  ABFT42_RS05815 (ABFT42_05815) - 1165897..1166301 (-) 405 Protein_1079 ferritin-like domain-containing protein -
  ABFT42_RS05820 (ABFT42_05820) addB 1166485..1169985 (+) 3501 WP_413287122.1 helicase-exonuclease AddAB subunit AddB Machinery gene
  ABFT42_RS05825 (ABFT42_05825) addA 1169972..1173676 (+) 3705 WP_413287123.1 helicase-exonuclease AddAB subunit AddA Machinery gene
  ABFT42_RS05830 (ABFT42_05830) sbcD 1173750..1174928 (+) 1179 WP_168747368.1 exonuclease subunit SbcD -
  ABFT42_RS05835 (ABFT42_05835) sbcC 1174925..1178317 (+) 3393 WP_413287124.1 exonuclease subunit SbcC -
  ABFT42_RS05840 (ABFT42_05840) hlpB 1178331..1178633 (+) 303 WP_229147779.1 HNH nuclease-like protein HlpB -

Sequence

Protein


Download         Length: 1234 a.a.        Molecular weight: 141627.07 Da        Isoelectric Point: 5.1039

>NTDB_id=1090294 ABFT42_RS05825 WP_413287123.1 1169972..1173676(+) (addA) [Bacillus mojavensis strain C3]
MNIPKPADSTWTDDQWNAIVSTGQDILVAAAAGSGKTAVLVERMIRKITAEEHPIDVDRLLVVTFTNASAAEMKHRIADA
LEKELVKRPGSLHIRRQLSLLNRASISTLHSFCLQVLKKYYYLIDLDPGFRIADQTEGELLGDEVLDELFEDEYAKGDKA
FFELVDRYTTDRHDLDLQYLVKQVYEYSRSHPDPETWLKSFVHLYDVTEKSAIEELPFYPYVKEDIAMVLEGAKEKLQRA
FELTKAPGGPAPRAENFLDDLAQIDDLIQHQDDFNELYKRVPAVSFKRAKAVKGDEFDPVLLDEATDLRNGAKKLLEKLK
IDYFTRSPEQHLKSLADMKPVIETLVQLVISYGKRFEAAKQEKSLIDFSDLEHYCLSILTAVNEKGEREPSEAARFYQEQ
FHEVLVDEYQDTNLVQESILQLVTSGPEETGNLFMVGDVKQSIYRFRLAEPLLFLSKYKRFTESGEGSGRKIDLNKNFRS
RADILDSTNFLFKQLMGGKIGEVDYDEQAELKLGAAYPENNETETELLLIDKSEETDAGEEAEELETVQFEAKAIAKEIR
KLISSPFKVYDGKTKTHRNIQYRDIVILLRSMPWAPQIMEELRAQGIPVYANLTSGYFDAVEVSVVLSVLKVIDNPYQDI
PLASVLRSPIVGADENELSLIRLENKKAPYYEAMKDYLAAGDRNDELYQKLQTFYGHLQKWRAFSKNHSVSELIWEVYRD
TKYMDYVGGMPGGKQRQANLRVLYDRARQYESTAFRGLFRFLRFIERMQERGDDLGTARALSEQEDVVRLMTIHSSKGLE
FPVVFTAGLGRNFNMMDLNKSYLLDKELGFGTKFIHPEWRISYPTLPLIAMKKKMRRELLSEELRVLYVALTRAKEKLFL
VGSCKDHQKQLAKWQASASQADWLLPEFDRYQAKTYLDFIGPALVRHRDLESLAEGGAAAASDISGHPARFAIQMIHSYD
LLDDELEERLEEKSERLEAIRRGEPVPGPFAFEEKARERLAWKYSHHEVTQIRTKQSVSEIKRRREYEDEYSGRAPVTPA
DGSILYRRPAFMMKKGLTAAEKGTAMHTVMQHIPLTHVPTTEEAERTVQHLYEKELLTEEQQSAIDIEEIVQFFHTEIGE
KLICAKWKDREVPFSLALPAKEIYPDAEEADEPLLVQGIIDCLYETEDGLYLLDYKTDRIEGKFQHGFEGAAPILKKRYE
TQIQLYTRAVEQIAQTKVKGCALYFFDGGHILTL

Nucleotide


Download         Length: 3705 bp        

>NTDB_id=1090294 ABFT42_RS05825 WP_413287123.1 1169972..1173676(+) (addA) [Bacillus mojavensis strain C3]
ATGAACATTCCTAAACCGGCAGACAGCACATGGACAGATGACCAGTGGAATGCCATCGTCTCAACCGGCCAGGATATCCT
TGTGGCAGCGGCGGCAGGATCTGGTAAAACGGCTGTTCTTGTGGAAAGAATGATCCGGAAAATCACGGCGGAAGAACATC
CGATCGATGTAGACCGGCTTCTTGTTGTCACCTTTACAAACGCCTCAGCTGCAGAGATGAAACACCGGATTGCGGATGCG
CTGGAAAAAGAGCTGGTAAAACGCCCAGGTTCGCTGCATATCCGTCGCCAGCTGTCGCTGTTAAACCGGGCCAGCATTTC
AACGCTTCATTCGTTTTGCCTGCAGGTGCTGAAAAAATATTACTACCTGATTGATCTTGATCCGGGCTTTCGGATTGCTG
ATCAGACAGAAGGCGAACTTCTCGGGGACGAGGTGCTTGATGAGCTGTTTGAGGATGAATATGCAAAGGGCGATAAGGCG
TTTTTTGAGCTTGTCGACCGATACACAACTGACCGGCATGACTTAGATCTGCAATATCTCGTGAAACAGGTGTATGAGTA
TTCTCGTTCCCACCCAGATCCGGAAACATGGCTGAAAAGCTTTGTTCATTTGTATGATGTAACAGAAAAGAGCGCGATCG
AGGAACTGCCGTTTTATCCATATGTCAAAGAAGATATTGCGATGGTGCTGGAGGGAGCAAAGGAAAAGCTTCAGCGTGCT
TTTGAGCTGACAAAAGCGCCGGGCGGACCTGCGCCACGCGCTGAAAATTTTCTCGATGATCTCGCGCAGATTGATGACCT
GATTCAGCATCAGGATGACTTCAATGAACTTTATAAGCGCGTGCCCGCTGTCTCCTTTAAGCGTGCAAAAGCGGTGAAAG
GGGACGAGTTTGACCCCGTCTTGCTTGATGAAGCGACAGACTTGAGAAATGGCGCCAAAAAACTCCTTGAAAAACTCAAA
ATCGATTACTTCACCAGAAGCCCGGAGCAGCATTTGAAAAGTTTGGCTGACATGAAGCCTGTCATTGAAACACTCGTACA
GCTTGTCATCAGCTACGGGAAACGGTTTGAAGCCGCAAAGCAGGAGAAATCGCTCATTGATTTTTCTGATTTGGAGCATT
ATTGCCTGTCCATCTTGACCGCTGTGAACGAAAAAGGTGAACGTGAACCAAGTGAGGCGGCAAGATTTTATCAGGAGCAG
TTTCATGAGGTGCTCGTCGATGAATATCAGGATACCAACCTGGTGCAGGAATCCATTCTTCAGCTCGTCACAAGCGGGCC
GGAAGAGACAGGAAACCTGTTTATGGTAGGAGATGTCAAGCAGTCGATTTATCGATTCAGGCTTGCGGAGCCGCTCCTTT
TTCTGTCTAAGTACAAACGGTTTACAGAGAGCGGAGAAGGGTCGGGCCGGAAAATCGACTTGAACAAAAACTTTCGAAGC
AGGGCTGATATTTTAGACAGCACAAACTTTTTGTTCAAACAGCTGATGGGCGGCAAAATCGGCGAGGTCGATTATGATGA
ACAGGCTGAACTGAAACTCGGCGCGGCGTATCCGGAAAACAATGAAACGGAAACCGAGCTGCTGCTGATTGACAAGTCCG
AGGAGACGGACGCAGGAGAGGAAGCGGAAGAGCTGGAAACGGTACAGTTCGAAGCAAAAGCGATCGCCAAGGAAATACGC
AAGCTGATCTCATCGCCATTTAAGGTATATGACGGGAAAACGAAGACACACCGCAATATTCAATACCGCGATATCGTGAT
TTTGCTGCGATCCATGCCGTGGGCGCCGCAAATCATGGAAGAGCTGAGAGCTCAGGGCATACCGGTTTACGCCAATTTAA
CATCAGGCTACTTTGATGCGGTGGAAGTGTCTGTCGTGCTTTCTGTCCTGAAGGTGATTGATAACCCGTATCAGGATATT
CCGCTTGCTTCCGTTCTGCGTTCACCGATTGTCGGTGCTGACGAGAACGAACTGTCTTTGATCCGGCTTGAAAACAAAAA
GGCGCCGTACTATGAAGCAATGAAAGACTATCTGGCAGCCGGCGACCGAAACGATGAGCTTTATCAAAAGCTGCAAACGT
TTTACGGGCATTTGCAAAAATGGCGCGCATTTTCGAAAAACCATTCGGTGTCCGAGCTGATCTGGGAAGTGTACCGCGAC
ACAAAGTATATGGATTATGTTGGCGGAATGCCAGGCGGAAAACAGCGGCAGGCTAACTTGCGCGTCCTCTATGACCGGGC
ACGCCAATATGAATCGACGGCATTTCGCGGCCTGTTTCGTTTCTTGAGGTTTATCGAACGCATGCAGGAACGCGGCGATG
ACCTGGGAACGGCGAGAGCGTTAAGCGAACAGGAAGATGTTGTCCGCTTAATGACCATTCACAGCAGCAAAGGGCTCGAA
TTTCCAGTCGTCTTCACAGCGGGACTTGGCCGGAATTTCAATATGATGGATCTCAACAAATCCTATCTGCTGGACAAGGA
GCTTGGGTTTGGCACGAAATTTATCCATCCTGAATGGCGCATCAGCTATCCGACGCTTCCGCTCATCGCGATGAAGAAAA
AAATGCGCAGGGAGCTGCTGTCTGAGGAATTGCGTGTGCTCTATGTTGCTTTAACGCGGGCAAAGGAGAAGCTGTTTCTT
GTCGGTTCATGCAAGGATCACCAGAAACAGCTGGCAAAATGGCAGGCATCGGCATCCCAAGCTGACTGGCTCCTGCCGGA
ATTTGATCGCTATCAGGCGAAAACGTATTTGGATTTCATCGGACCTGCTCTTGTCAGACACAGAGACTTGGAGTCTTTGG
CTGAGGGCGGTGCGGCAGCGGCTTCTGATATATCAGGCCATCCGGCCCGCTTTGCTATTCAAATGATCCATTCATATGAT
CTGCTTGATGATGAGCTGGAAGAGAGACTGGAAGAAAAAAGCGAACGTCTTGAAGCGATCCGCCGCGGGGAACCGGTTCC
CGGCCCGTTTGCATTTGAGGAAAAAGCTCGCGAGCGGCTGGCCTGGAAGTATTCGCATCACGAGGTGACACAAATTCGCA
CGAAGCAGTCTGTTTCTGAGATTAAGAGAAGAAGAGAGTACGAAGACGAATACAGCGGACGGGCGCCCGTTACACCGGCT
GATGGAAGCATTCTGTACAGACGCCCGGCTTTCATGATGAAAAAAGGCCTGACAGCAGCAGAGAAAGGAACAGCTATGCA
TACGGTCATGCAGCATATTCCGCTGACACATGTGCCGACGACAGAAGAAGCGGAGCGGACAGTGCAGCATCTTTATGAAA
AAGAGCTGCTCACCGAAGAACAGCAATCCGCTATCGATATTGAGGAAATCGTTCAATTTTTCCATACGGAAATTGGCGAA
AAACTGATCTGCGCTAAATGGAAGGACCGTGAAGTTCCATTCAGTTTGGCGCTTCCGGCAAAAGAGATTTATCCTGATGC
GGAAGAGGCGGATGAGCCGCTATTGGTGCAGGGGATCATCGATTGTCTCTACGAAACAGAGGACGGCTTATATTTGCTGG
ACTATAAAACAGACCGGATTGAGGGGAAATTCCAGCACGGCTTTGAAGGAGCAGCCCCGATCCTGAAAAAGAGATATGAA
ACGCAAATTCAGCTGTACACAAGGGCCGTTGAACAAATTGCTCAAACGAAGGTAAAAGGGTGTGCGCTTTATTTCTTTGA
CGGAGGGCATATTCTGACTTTATAA

Domains

Predicted by InterproScan.

PD-(D/E)XK nuclease superfamily (PDDEXK_1)

(1055-1225)

UvrD/REP helicase N-terminal domain (UvrD-helicase)

(11-461)

UvrD-like helicase C-terminal domain (UvrD_C)

(472-881)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  addA Bacillus subtilis subsp. subtilis str. 168

93.436

100

0.934

Multiple sequence alignment