Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB/pilB1   Type   Machinery gene
Locus tag   ACLLO5_RS14115 Genome accession   NZ_CP178454
Coordinates   3184410..3186422 (-) Length   670 a.a.
NCBI ID   WP_413171149.1    Uniprot ID   -
Organism   Anabaena azotica SJ-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3179410..3191422
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACLLO5_RS14090 (ACLLO5_14090) - 3179625..3179894 (-) 270 WP_413171144.1 hypothetical protein -
  ACLLO5_RS14095 (ACLLO5_14095) bioU 3180012..3181007 (+) 996 WP_413171145.1 (S)-8-amino-7-oxononanoate synthase BioU -
  ACLLO5_RS14100 (ACLLO5_14100) - 3181082..3181630 (-) 549 WP_413171146.1 hypothetical protein -
  ACLLO5_RS14105 (ACLLO5_14105) - 3181766..3182989 (-) 1224 WP_413171147.1 type II secretion system F family protein -
  ACLLO5_RS14110 (ACLLO5_14110) pilT 3183121..3184245 (-) 1125 WP_413171148.1 type IV pilus twitching motility protein PilT Machinery gene
  ACLLO5_RS14115 (ACLLO5_14115) pilB/pilB1 3184410..3186422 (-) 2013 WP_413171149.1 GspE/PulE family protein Machinery gene
  ACLLO5_RS14120 (ACLLO5_14120) grpE 3186830..3187573 (+) 744 WP_413171150.1 nucleotide exchange factor GrpE -
  ACLLO5_RS14125 (ACLLO5_14125) dnaK 3188073..3190061 (+) 1989 WP_413171151.1 molecular chaperone DnaK -

Sequence

Protein


Download         Length: 670 a.a.        Molecular weight: 75438.35 Da        Isoelectric Point: 5.3495

>NTDB_id=1089671 ACLLO5_RS14115 WP_413171149.1 3184410..3186422(-) (pilB/pilB1) [Anabaena azotica SJ-1]
MTYSSPQRPSTALTTRTQFSPFGNKLVQSGYVNTEQMKQALIESRKSGKMLTDVLESITGQQLSPEFIREYKKQHLFELK
ILYGVESLDPEVNQIGNLMVGQLIDSLIPVDVCRRHRLVPLSKREDQTPPYVLVAMVEPDNLDASDDLNRILRNKNLSLQ
RMVITQEDYQHLINKYLDELAVREKEKEQKRDTDINQDIKYLEDQDIEEVGEDKDADINASIKDAEDAPIIKLVNRILFK
ALQEKVSDIHIEPQEENLRIRFRKDGVLREAFDPVPKKIIPAVTARFKIISNLDIAERRLPQDGRIRRLFEGRKVDFRVN
TLPSRYGEKVVLRILDNSSTQLGLDKLITDPETLHIVQDMVSKPFGLILVTGPTGSGKTTSLYSALAEKNDPGINISTVE
DPIEYSLPGITQVQVIREKGLDFSTALRAFLRQDPDVLLVGETRDKETAKTAIEAALTGHLVLTTLHTNDAPGAIARLGE
MGIEPFMVSSSLIGVLAQRLVRRVCSQCRIPYTPTTEELARYGLSASKETSVTFYKANSLPPESIIEAKTKNQLCPACNG
IGYKGRCGVYEVMRVTENLQTLINQEAPTERIKEVAVEEGMKTLLAYSLDLVRQGSTTLEEVERVTFTDTGLEAELKAKR
KSGLTCRTCDATLQPEWLDCPYCMTPRFQD

Nucleotide


Download         Length: 2013 bp        

>NTDB_id=1089671 ACLLO5_RS14115 WP_413171149.1 3184410..3186422(-) (pilB/pilB1) [Anabaena azotica SJ-1]
ATGACTTACTCGTCACCACAACGGCCTAGCACCGCGTTAACTACAAGAACACAGTTTTCGCCCTTTGGCAACAAGCTAGT
GCAATCTGGCTATGTCAACACTGAACAGATGAAACAGGCACTAATAGAGAGCCGGAAATCTGGCAAAATGCTGACAGATG
TCCTAGAGTCAATTACTGGGCAACAACTATCACCAGAGTTTATTCGGGAATACAAGAAACAACATCTATTTGAACTAAAA
ATATTATATGGCGTTGAATCACTAGATCCAGAAGTCAATCAAATTGGCAATCTGATGGTTGGACAATTGATTGATAGTCT
GATACCCGTGGATGTCTGCCGTCGTCATCGTTTGGTTCCGCTATCGAAACGGGAAGACCAAACGCCACCTTATGTTCTAG
TGGCAATGGTTGAACCAGATAATCTGGATGCTTCCGATGACCTGAACCGGATCTTGCGTAACAAAAACTTATCCTTGCAG
CGGATGGTGATTACTCAAGAAGATTACCAGCATCTGATCAACAAATATTTAGATGAGTTGGCTGTTCGGGAAAAGGAAAA
AGAACAGAAAAGAGATACAGATATTAATCAGGATATAAAATACCTGGAAGATCAGGATATAGAGGAAGTTGGTGAGGACA
AGGATGCTGATATAAACGCATCCATTAAGGATGCTGAAGATGCACCCATTATTAAACTTGTCAACAGAATCCTGTTTAAA
GCTTTGCAGGAAAAGGTTTCGGATATTCATATCGAACCACAAGAGGAAAACTTGCGGATTCGCTTCCGTAAGGATGGGGT
ACTGCGTGAGGCTTTTGACCCTGTACCTAAAAAAATCATTCCGGCGGTGACAGCCCGATTTAAGATCATTTCCAATCTTG
ATATCGCGGAAAGGCGTTTACCTCAAGATGGTCGTATCCGTCGTTTGTTTGAAGGACGGAAAGTTGATTTCCGGGTGAAT
ACCTTACCCAGTCGCTATGGGGAAAAGGTGGTATTACGGATTCTGGATAACTCTTCTACCCAACTAGGGTTGGATAAGTT
AATTACCGATCCAGAGACTTTGCATATTGTCCAGGATATGGTCAGTAAGCCCTTTGGATTGATTTTGGTGACAGGTCCAA
CTGGTTCTGGTAAAACGACTTCGTTGTATTCAGCATTGGCAGAAAAGAATGATCCAGGAATTAACATCAGTACGGTAGAA
GACCCGATTGAATATAGTTTGCCAGGGATTACCCAAGTACAGGTAATTCGGGAAAAAGGTCTGGATTTTTCGACAGCTTT
AAGAGCCTTCTTACGACAAGATCCTGATGTGTTGCTTGTGGGGGAAACACGGGATAAAGAAACGGCAAAAACAGCGATTG
AAGCGGCTTTAACAGGTCACTTAGTATTAACTACCTTACACACTAATGATGCTCCTGGTGCGATCGCACGTCTGGGAGAA
ATGGGAATTGAACCATTCATGGTTTCCAGTTCCCTAATTGGTGTTTTAGCACAACGTTTGGTGCGCCGTGTCTGTTCACA
ATGTCGAATTCCCTACACTCCCACTACTGAAGAACTGGCTCGTTATGGTCTGTCAGCATCCAAAGAAACATCAGTCACTT
TTTACAAAGCTAACTCCCTACCACCAGAATCCATAATTGAAGCCAAAACTAAAAATCAACTCTGTCCAGCTTGTAATGGC
ATTGGCTATAAAGGACGTTGTGGTGTTTATGAAGTCATGCGAGTCACAGAAAACCTGCAAACTCTCATCAACCAAGAAGC
ACCCACAGAACGCATCAAAGAAGTAGCTGTAGAAGAAGGCATGAAAACCTTGCTTGCTTACAGTCTAGATTTAGTGCGTC
AAGGTTCTACAACTCTGGAAGAAGTAGAGCGCGTAACCTTCACTGATACAGGTTTAGAAGCTGAGTTAAAAGCCAAACGT
AAGAGCGGTCTAACTTGTAGAACTTGTGATGCCACATTACAACCGGAATGGCTCGATTGTCCCTACTGTATGACACCTCG
TTTTCAAGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(72-179)

Type II/IV secretion system protein (T2SSE)

(236-503)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB/pilB1 Synechocystis sp. PCC 6803

64.881

100

0.651

Multiple sequence alignment