Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   ACAM23_RS00340 Genome accession   NZ_AP031394
Coordinates   57977..59338 (+) Length   453 a.a.
NCBI ID   WP_074017595.1    Uniprot ID   A0A237IXT5
Organism   Streptococcus pneumoniae strain 16P28     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 52977..64338
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACAM23_RS00280 - 53003..53120 (+) 118 Protein_49 dihydrodipicolinate reductase -
  ACAM23_RS00285 (SPNE16P28_00530) - 53101..53304 (+) 204 WP_001091118.1 hypothetical protein -
  ACAM23_RS00290 (SPNE16P28_00540) - 53307..53657 (+) 351 WP_050218371.1 hypothetical protein -
  ACAM23_RS00295 (SPNE16P28_00550) - 53667..54083 (+) 417 WP_050211460.1 phage holin family protein -
  ACAM23_RS00300 (SPNE16P28_00560) - 54087..54419 (+) 333 WP_001186242.1 phage holin -
  ACAM23_RS00305 (SPNE16P28_00570) lytA 54423..55379 (+) 957 WP_050218372.1 N-acetylmuramoyl-L-alanine amidase LytA -
  ACAM23_RS00310 (SPNE16P28_00580) - 55599..55778 (-) 180 WP_001209433.1 hypothetical protein -
  ACAM23_RS00315 (SPNE16P28_00590) - 55920..56069 (-) 150 WP_001030863.1 hypothetical protein -
  ACAM23_RS00320 (SPNE16P28_00600) tadA 56350..56817 (+) 468 WP_000291870.1 tRNA adenosine(34) deaminase TadA -
  ACAM23_RS00330 (SPNE16P28_00610) - 57003..57446 (+) 444 WP_000701992.1 dUTP diphosphatase -
  ACAM23_RS00335 (SPNE16P28_00620) - 57448..57963 (+) 516 WP_001842035.1 histidine phosphatase family protein -
  ACAM23_RS00340 (SPNE16P28_00630) radA 57977..59338 (+) 1362 WP_074017595.1 DNA repair protein RadA Machinery gene
  ACAM23_RS00345 (SPNE16P28_00640) - 59411..59908 (+) 498 WP_001809263.1 carbonic anhydrase -
  ACAM23_RS00350 (SPNE16P28_00650) - 59933..60747 (+) 815 Protein_62 PrsW family intramembrane metalloprotease -
  ACAM23_RS00355 (SPNE16P28_00660) - 60892..61860 (+) 969 WP_000010163.1 ribose-phosphate diphosphokinase -
  ACAM23_RS00360 - 61994..62275 (-) 282 Protein_64 ISL3 family transposase -
  ACAM23_RS00365 - 62402..63309 (-) 908 Protein_65 Rpn family recombination-promoting nuclease/putative transposase -

Sequence

Protein


Download         Length: 453 a.a.        Molecular weight: 49432.95 Da        Isoelectric Point: 6.5137

>NTDB_id=108940 ACAM23_RS00340 WP_074017595.1 57977..59338(+) (radA) [Streptococcus pneumoniae strain 16P28]
MAKKKATFVCQNCGYNSPKYLGRCPNCGSWSSFVEEVEVAEVKNARVSLTGEKTKPMKLAEVTSINVNRTKTEMEEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSTQLSQVGTVLYVSGEESAQQIKLRAERLGDIDSEFYLYAETNMQSVRAEVE
RIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELMQLAKTNNIAIFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFEMQSGGLVEVLNPSQVFLEERLDGATGSSIVVTMEGTRPILAEVQALVTPTMFGNAK
RTTTGLDFNRASLIMAVLEKRAGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKDKPTNPQECFVGELGLTGEIR
RVNRIEQRINEAAKLGFTKIYVPKNSLTGITLPKEIQVIGVTTIQEVLKKVFA

Nucleotide


Download         Length: 1362 bp        

>NTDB_id=108940 ACAM23_RS00340 WP_074017595.1 57977..59338(+) (radA) [Streptococcus pneumoniae strain 16P28]
ATCGCAAAGAAAAAAGCGACATTTGTATGTCAAAATTGTGGGTATAATTCCCCTAAATATCTGGGACGTTGCCCCAACTG
TGGGTCTTGGTCTTCTTTTGTGGAAGAGGTTGAGGTTGCCGAGGTCAAGAATGCGCGTGTGTCCTTGACAGGTGAGAAAA
CCAAGCCCATGAAACTAGCTGAGGTGACTTCCATCAATGTCAATCGAACCAAGACGGAGATGGAGGAATTCAACCGTGTG
CTTGGAGGCGGAGTGGTACCAGGAAGTCTCGTCCTCATCGGTGGGGATCCTGGGATTGGGAAATCAACTCTTCTCCTACA
AGTCTCAACCCAGTTGTCCCAAGTGGGGACAGTTCTCTATGTCAGTGGGGAGGAGTCTGCCCAGCAGATTAAACTACGTG
CAGAGCGCTTAGGTGATATTGATAGTGAGTTTTATCTCTATGCAGAGACCAATATGCAGAGTGTTCGTGCAGAAGTGGAG
CGTATCCAGCCAGACTTTCTCATTATTGATTCCATCCAGACCATCATGTCTCCTGAGATTTCAGGGGTGCAGGGGTCTGT
TTCTCAGGTACGTGAAGTGACCGCTGAACTCATGCAGTTGGCCAAGACCAATAACATTGCCATCTTTATCGTAGGTCATG
TGACCAAAGAAGGAACCTTGGCTGGGCCTCGTATGTTGGAGCATATGGTGGATACGGTGCTTTACTTTGAAGGGGAGCGT
CACCACACCTTTCGTATTTTGAGAGCGGTCAAAAATCGTTTTGGTTCCACTAATGAGATTGGGATTTTTGAGATGCAGTC
GGGCGGCTTGGTTGAGGTACTCAATCCGAGTCAAGTTTTCCTAGAAGAGCGTTTGGATGGGGCGACTGGTTCCTCCATCG
TTGTAACCATGGAAGGGACGCGTCCGATTTTGGCGGAGGTTCAGGCTTTGGTAACACCGACCATGTTTGGAAATGCCAAG
CGTACTACGACAGGACTTGATTTTAACCGTGCTAGCTTGATTATGGCTGTTTTGGAAAAACGGGCAGGGCTTCTCTTGCA
AAATCAGGATGCCTATCTCAAATCTGCTGGTGGTGTTAAATTGGATGAACCTGCCATTGACTTGGCTGTTGCAGTTGCTA
TTGCTTCGAGCTACAAAGACAAGCCAACTAATCCTCAGGAATGTTTTGTCGGAGAACTGGGCTTGACAGGAGAGATTCGG
CGCGTGAATCGTATTGAGCAACGCATCAACGAAGCTGCTAAACTGGGCTTTACTAAGATTTATGTACCTAAGAATTCCTT
GACAGGAATCACTCTGCCTAAGGAAATTCAGGTCATTGGCGTGACAACGATTCAGGAAGTCTTGAAAAAGGTCTTTGCAT
AA

Domains

Predicted by InterProScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(63-218)

Subunit ChlI of Mg-chelatase (ChlI)

(360-425)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  PDB 8RXC

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus pneumoniae Rx1

100

100

1
  radA Streptococcus pneumoniae D39

100

100

1
  radA Streptococcus pneumoniae R6

100

100

1
  radA Streptococcus pneumoniae TIGR4

100

100

1
  radA Streptococcus mitis NCTC 12261

99.558

100

0.996
  radA Streptococcus mitis SK321

99.558

100

0.996
  radA Bacillus subtilis subsp. subtilis str. 168

63.135

100

0.631

Multiple sequence alignment