Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF/pilF2   Type   Machinery gene
Locus tag   ACIRXM_RS05290 Genome accession   NZ_CP173234
Coordinates   1029731..1030273 (+) Length   180 a.a.
NCBI ID   WP_046097864.1    Uniprot ID   A0A0F5EZV4
Organism   Avibacterium paragallinarum strain npAP/NC-USA/20240110/S1-1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1024731..1035273
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACIRXM_RS05265 (ACIRXM_05270) - 1025212..1026141 (+) 930 WP_410687618.1 phosphatidate cytidylyltransferase -
  ACIRXM_RS05270 (ACIRXM_05275) mog 1026160..1026744 (+) 585 WP_410687620.1 molybdopterin adenylyltransferase -
  ACIRXM_RS05275 (ACIRXM_05280) glnB 1026764..1027102 (+) 339 WP_103854008.1 nitrogen regulatory protein P-II -
  ACIRXM_RS05280 (ACIRXM_05285) - 1027102..1028154 (+) 1053 WP_410687621.1 AI-2E family transporter -
  ACIRXM_RS05285 (ACIRXM_05290) - 1028528..1029697 (+) 1170 WP_410687623.1 bifunctional tRNA (adenosine(37)-C2)-methyltransferase TrmG/ribosomal RNA large subunit methyltransferase RlmN -
  ACIRXM_RS05290 (ACIRXM_05295) pilF/pilF2 1029731..1030273 (+) 543 WP_046097864.1 type IV pilus biogenesis/stability protein PilW Machinery gene
  ACIRXM_RS05295 (ACIRXM_05300) dprA 1030418..1031518 (+) 1101 WP_035687998.1 DNA-processing protein DprA Machinery gene
  ACIRXM_RS05300 (ACIRXM_05305) rsmD 1031515..1032123 (-) 609 WP_410687624.1 16S rRNA (guanine(966)-N(2))-methyltransferase RsmD -
  ACIRXM_RS05305 (ACIRXM_05310) ftsY 1032279..1033772 (+) 1494 WP_410687625.1 signal recognition particle-docking protein FtsY -
  ACIRXM_RS05310 (ACIRXM_05315) - 1033986..1034126 (+) 141 WP_410687626.1 hypothetical protein -

Sequence

Protein


Download         Length: 180 a.a.        Molecular weight: 20689.64 Da        Isoelectric Point: 8.0752

>NTDB_id=1071073 ACIRXM_RS05290 WP_046097864.1 1029731..1030273(+) (pilF/pilF2) [Avibacterium paragallinarum strain npAP/NC-USA/20240110/S1-1]
MKIYQCILSILFSLFLSACVSSPLNKQDFDKQQAAKARVELGIGYLEQNELGLAKQNLDKALAYAPNYYLVHSALAYFYQ
LQGENTRAENAYLKALKLDDKQGDVFNNYAVFLCKQGQFEQAYLRFEQALATPNYYQQADTYENIVLCALAEKNAIRYEK
YKGLLEKLDPKRNQKTNVIH

Nucleotide


Download         Length: 543 bp        

>NTDB_id=1071073 ACIRXM_RS05290 WP_046097864.1 1029731..1030273(+) (pilF/pilF2) [Avibacterium paragallinarum strain npAP/NC-USA/20240110/S1-1]
ATGAAAATTTATCAATGTATCTTAAGCATTCTTTTTTCTCTCTTTCTTTCCGCTTGTGTGTCTTCGCCCTTAAATAAGCA
AGACTTTGATAAGCAGCAAGCCGCAAAGGCGAGAGTGGAGCTTGGCATTGGCTATCTTGAGCAAAATGAACTTGGGCTGG
CGAAGCAAAATTTAGACAAAGCTTTAGCTTATGCCCCGAATTATTATTTAGTGCATTCAGCGTTGGCATATTTTTATCAG
CTTCAAGGGGAGAATACTCGTGCGGAAAATGCGTATTTGAAAGCCCTAAAACTCGACGATAAACAAGGCGATGTATTTAA
TAATTATGCCGTCTTTTTGTGCAAACAAGGACAATTCGAGCAGGCTTATCTACGATTTGAACAAGCTCTCGCCACACCTA
ATTATTATCAGCAAGCGGATACTTACGAGAATATTGTACTTTGTGCGTTAGCGGAGAAAAATGCAATACGTTATGAAAAA
TATAAAGGCTTGCTGGAGAAATTAGATCCTAAAAGAAATCAAAAGACAAATGTTATTCATTAA

Domains

Predicted by InterproScan.

Tetratricopeptide repeat (TPR_17)

(91-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F5EZV4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF/pilF2 Haemophilus influenzae Rd KW20

50.877

95

0.483
  pilF Glaesserella parasuis strain SC1401

42.857

100

0.433

Multiple sequence alignment