Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACFWHB_RS01970 Genome accession   NZ_CP172298
Coordinates   396992..398431 (+) Length   479 a.a.
NCBI ID   WP_163137981.1    Uniprot ID   A0AAW9YBY1
Organism   Aeromonas sp. A-8     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 385153..396926 396992..398431 flank 66


Gene organization within MGE regions


Location: 385153..398431
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFWHB_RS01885 (ACFWHB_01885) - 385153..385560 (-) 408 Protein_354 transposase -
  ACFWHB_RS01890 (ACFWHB_01890) - 385594..386651 (-) 1058 Protein_355 IS3 family transposase -
  ACFWHB_RS01895 (ACFWHB_01895) - 386667..387829 (+) 1163 WP_395948442.1 IS3 family transposase -
  ACFWHB_RS01900 (ACFWHB_01900) - 387837..387923 (-) 87 Protein_357 transposase -
  ACFWHB_RS01905 (ACFWHB_01905) - 387986..388725 (-) 740 Protein_358 IS3 family transposase -
  ACFWHB_RS01910 (ACFWHB_01910) murA 388846..390102 (-) 1257 WP_106885623.1 UDP-N-acetylglucosamine 1-carboxyvinyltransferase -
  ACFWHB_RS01915 (ACFWHB_01915) ibaG 390119..390376 (-) 258 WP_005306001.1 BolA family iron metabolism protein IbaG -
  ACFWHB_RS01920 (ACFWHB_01920) - 390376..390648 (-) 273 WP_042649054.1 lipid asymmetry maintenance protein MlaB -
  ACFWHB_RS01925 (ACFWHB_01925) mlaC 390645..391280 (-) 636 WP_042649053.1 phospholipid-binding protein MlaC -
  ACFWHB_RS01930 (ACFWHB_01930) mlaD 391283..391783 (-) 501 WP_106885624.1 outer membrane lipid asymmetry maintenance protein MlaD -
  ACFWHB_RS01935 (ACFWHB_01935) mlaE 391791..392570 (-) 780 WP_025328536.1 lipid asymmetry maintenance ABC transporter permease subunit MlaE -
  ACFWHB_RS01940 (ACFWHB_01940) mlaF 392570..393370 (-) 801 WP_025328535.1 phospholipid ABC transporter ATP-binding protein MlaF -
  ACFWHB_RS01945 (ACFWHB_01945) - 393577..394572 (+) 996 WP_163148895.1 KpsF/GutQ family sugar-phosphate isomerase -
  ACFWHB_RS01950 (ACFWHB_01950) kdsC 394572..395126 (+) 555 WP_042649051.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACFWHB_RS01955 (ACFWHB_01955) lptC 395123..395683 (+) 561 WP_042649050.1 LPS export ABC transporter periplasmic protein LptC -
  ACFWHB_RS01960 (ACFWHB_01960) lptA 395670..396188 (+) 519 WP_025328531.1 lipopolysaccharide transport periplasmic protein LptA -
  ACFWHB_RS01965 (ACFWHB_01965) lptB 396201..396926 (+) 726 WP_395949364.1 LPS export ABC transporter ATP-binding protein -
  ACFWHB_RS01970 (ACFWHB_01970) rpoN/rpoN1 396992..398431 (+) 1440 WP_163137981.1 RNA polymerase factor sigma-54 Machinery gene

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53762.73 Da        Isoelectric Point: 4.4177

>NTDB_id=1066096 ACFWHB_RS01970 WP_163137981.1 396992..398431(+) (rpoN/rpoN1) [Aeromonas sp. A-8]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENEAQETSPIEPQDASQLDSSDAMAQDKMP
DELPVDTTWDEVYSAGTAQSAGPIHDGEDSVYQGETTENLQDYLLWQMRLTPFSDLDAAIALAIIDAIDESGYLTVSLED
IQAAVSSEEVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVIREHMDLLGNRDYRTLARK
TRLKESDLKEVLDLIQQLEPRPGNGIIQSESQYVIPDVSVVKKGAKWVVELNPDSAPRIRVNEAYAAMARNARSSTDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=1066096 ACFWHB_RS01970 WP_163137981.1 396992..398431(+) (rpoN/rpoN1) [Aeromonas sp. A-8]
ATGAAGCCGACATTACAGTTGCGCCTCGGTCAGTCACTGACCATGACGCCACAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACCCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGATAATAACCCCTTGCTGGAGCAGGAGGAGAATGAGG
CGCAGGAAACCAGCCCGATAGAGCCCCAGGACGCCAGTCAACTCGACTCCAGCGACGCCATGGCGCAGGATAAGATGCCG
GATGAACTGCCGGTGGACACCACCTGGGATGAGGTCTACTCCGCCGGTACGGCCCAGAGCGCCGGCCCCATCCATGATGG
GGAAGACAGCGTCTATCAGGGGGAAACCACCGAGAACCTGCAGGACTATCTGCTGTGGCAGATGCGCTTGACGCCGTTCA
GCGATCTGGATGCCGCCATCGCCCTCGCCATCATCGATGCCATCGATGAGTCCGGTTACCTGACCGTCTCTCTCGAAGAT
ATTCAGGCTGCAGTCAGCAGCGAAGAGGTAGAAGTTGAACTGGATGAAGTGGAAGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTTGGCATAGCCGCGCGCTCGGTGCAGGAGTGTCTGCTGATCCAGCTTGGCCAGTACTCGCCGGATCTGCCCT
GGCTCAATGAGGCAAGGGAGGTGATCCGCGAGCACATGGATCTGCTCGGCAACCGCGACTACCGCACCCTGGCACGCAAG
ACCCGGCTCAAGGAGAGCGACCTCAAGGAGGTGCTGGATCTTATCCAGCAGCTGGAACCCAGACCCGGCAATGGCATCAT
CCAGAGTGAATCTCAATACGTGATCCCGGACGTCTCCGTGGTCAAGAAGGGGGCTAAGTGGGTGGTTGAGCTCAACCCGG
ATTCGGCGCCGCGCATCCGTGTCAACGAGGCCTATGCGGCCATGGCACGCAATGCCCGCAGCAGTACGGATACCCAGTTC
ATCCGCTCCCATCTGCAGGAAGCCAAATGGTTCATCAAGAGCCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCCAG
CTGCATAGTCGAGCAGCAGCAGGGCTTCTTCGAATACGGTGAAGAGGCCATGAAACCCATGGTACTCAACGACATCGCCG
AGGCGGTGGAGATGCACGAGTCGACGATTTCACGGGTGACGACCCAGAAGTACATGCATACGCCCCGTGGCATCTTTGAA
CTGAAGTTTTTTTTCTCCAGTCATGTCAACACGGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCCCTGATCAA
GAAGCTGGTGACGGCCGAAAACCCGGCCAAACCGCTTAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

46.667

100

0.497

Multiple sequence alignment