Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACFX5Z_RS19930 Genome accession   NZ_CP170761
Coordinates   4338069..4339508 (-) Length   479 a.a.
NCBI ID   WP_206220873.1    Uniprot ID   -
Organism   Aeromonas dhakensis strain CWH5     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4333069..4344508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFX5Z_RS19900 (ACFX5Z_19900) - 4333344..4334084 (+) 741 WP_279452156.1 KDGP aldolase family protein -
  ACFX5Z_RS19905 (ACFX5Z_19905) - 4334189..4336093 (+) 1905 WP_279452155.1 transcription antiterminator -
  ACFX5Z_RS19910 (ACFX5Z_19910) npr 4336109..4336381 (-) 273 WP_005305943.1 PTS phosphocarrier protein NPr -
  ACFX5Z_RS19915 (ACFX5Z_19915) rapZ 4336402..4337268 (-) 867 WP_005305946.1 RNase adapter RapZ -
  ACFX5Z_RS19920 (ACFX5Z_19920) ptsN 4337311..4337757 (-) 447 WP_005305951.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACFX5Z_RS19925 (ACFX5Z_19925) hpf 4337760..4338047 (-) 288 WP_005305957.1 ribosome hibernation promoting factor -
  ACFX5Z_RS19930 (ACFX5Z_19930) rpoN/rpoN1 4338069..4339508 (-) 1440 WP_206220873.1 RNA polymerase factor sigma-54 Machinery gene
  ACFX5Z_RS19935 (ACFX5Z_19935) lptB 4339574..4340299 (-) 726 WP_005305964.1 LPS export ABC transporter ATP-binding protein -
  ACFX5Z_RS19940 (ACFX5Z_19940) lptA 4340303..4340830 (-) 528 WP_010635928.1 lipopolysaccharide transport periplasmic protein LptA -
  ACFX5Z_RS19945 (ACFX5Z_19945) lptC 4340817..4341377 (-) 561 WP_024943092.1 LPS export ABC transporter periplasmic protein LptC -
  ACFX5Z_RS19950 (ACFX5Z_19950) kdsC 4341374..4341928 (-) 555 WP_017766397.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACFX5Z_RS19955 (ACFX5Z_19955) - 4341928..4342923 (-) 996 WP_024943091.1 KpsF/GutQ family sugar-phosphate isomerase -
  ACFX5Z_RS19960 (ACFX5Z_19960) mlaF 4343131..4343931 (+) 801 WP_005305979.1 phospholipid ABC transporter ATP-binding protein MlaF -

Sequence

Protein


Download         Length: 479 a.a.        Molecular weight: 53878.85 Da        Isoelectric Point: 4.3584

>NTDB_id=1059319 ACFX5Z_RS19930 WP_206220873.1 4338069..4339508(-) (rpoN/rpoN1) [Aeromonas dhakensis strain CWH5]
MKPTLQLRLGQSLTMTPQLQQAIRLLQLSTLELQQEIQQALDNNPLLEQEENDVQETSPVDQVDVSQLDSSDAMAQDQMP
DELPVDTTWEEVYSAGTAQSAGPIHDGEDSIYQGETTENLQDYLLWQMRLTPFSDVDAAIALAIIDAIDESGYLTVSLED
IQTAVSSEDVEVELDEVEAVLKRVQHFDPVGIAARSVQECLLIQLGQYSPDLPWLNEAREVILEHMDLLGNRDYRTLARK
TKLKENDLKEVLDLIQQLEPRPGNGVIQSESQYVIPDVSVVKKGNKWVVELNPDSAPRIRVNETYAAMARQARTSQDTQF
IRSHLQEAKWFIKSLESRNDTLLKVASCIVEQQQGFFEYGEEAMKPMVLNDIAEAVEMHESTISRVTTQKYMHTPRGIFE
LKFFFSSHVNTEGGGECSSTAIRALIKKLVTAENPAKPLSDSKIADLLAEQGIMVARRTIAKYRESLLIPPSNQRKRLV

Nucleotide


Download         Length: 1440 bp        

>NTDB_id=1059319 ACFX5Z_RS19930 WP_206220873.1 4338069..4339508(-) (rpoN/rpoN1) [Aeromonas dhakensis strain CWH5]
ATGAAGCCGACATTACAGTTGCGTCTCGGTCAGTCCCTGACCATGACGCCGCAATTACAACAAGCGATTCGTCTGCTCCA
GCTCTCCACTCTGGAACTCCAGCAGGAAATTCAGCAAGCGCTCGACAACAACCCCTTGCTGGAGCAAGAGGAAAATGACG
TTCAGGAGACCAGCCCGGTCGACCAAGTGGACGTCAGCCAGCTCGATTCCAGCGATGCCATGGCCCAGGACCAGATGCCG
GACGAACTGCCGGTGGATACCACCTGGGAAGAGGTCTATTCGGCAGGCACGGCTCAGAGCGCAGGCCCCATCCATGACGG
GGAAGACAGCATCTATCAGGGTGAGACCACCGAGAACCTGCAGGACTACCTGTTGTGGCAGATGCGCTTGACCCCGTTCA
GCGACGTGGATGCCGCCATCGCCCTCGCCATCATAGATGCCATCGACGAATCCGGTTACCTGACGGTGTCGCTTGAGGAT
ATTCAGACGGCGGTGAGCAGCGAGGATGTCGAGGTGGAACTCGATGAAGTGGAGGCCGTGCTCAAGCGGGTGCAGCATTT
CGATCCGGTCGGCATCGCCGCCCGCTCGGTGCAGGAGTGCCTGCTGATCCAGCTGGGCCAGTACTCCCCTGATCTGCCCT
GGCTCAACGAAGCCAGGGAGGTGATCCTCGAGCACATGGATCTGCTCGGCAACCGCGATTACCGCACCCTGGCCCGCAAG
ACCAAGCTGAAGGAGAACGACCTCAAGGAGGTGCTGGATCTCATCCAGCAGCTGGAGCCGCGGCCCGGAAACGGCGTCAT
CCAGAGCGAATCCCAGTACGTGATCCCGGATGTTTCCGTGGTCAAGAAGGGCAACAAGTGGGTGGTCGAGCTCAACCCTG
ACTCTGCCCCGCGCATTCGGGTCAACGAGACCTATGCCGCCATGGCCAGACAGGCCCGCACCAGTCAGGATACCCAGTTC
ATCCGCTCCCACCTGCAGGAAGCCAAGTGGTTCATCAAGAGTCTGGAGAGTCGCAACGACACCCTGCTCAAGGTGGCCAG
CTGCATCGTCGAGCAGCAGCAGGGCTTTTTCGAATACGGTGAAGAGGCCATGAAACCCATGGTGCTCAACGACATCGCCG
AAGCGGTGGAGATGCACGAGTCGACCATCTCGCGGGTGACCACCCAGAAGTACATGCACACGCCGCGCGGCATCTTCGAA
CTGAAGTTTTTTTTCTCCAGCCATGTGAACACCGAGGGAGGAGGAGAGTGCTCATCGACCGCAATTCGCGCGCTCATCAA
GAAGCTGGTCACGGCGGAGAATCCCGCCAAGCCGCTAAGTGATAGCAAGATCGCGGATTTGCTGGCCGAACAGGGTATTA
TGGTGGCGAGACGCACGATCGCAAAGTACCGTGAATCCTTGTTGATCCCGCCATCCAATCAGCGCAAACGCCTGGTTTAG

Domains

Predicted by InterproScan.

Sigma-54, DNA binding domain (Sigma54_DBD)

(319-477)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-305)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.669

100

0.484

Multiple sequence alignment