Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACFJI2_RS29555 Genome accession   NZ_CP170549
Coordinates   6609355..6610845 (+) Length   496 a.a.
NCBI ID   WP_377703010.1    Uniprot ID   -
Organism   Pseudoduganella sp. UC29_71     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6604355..6615845
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFJI2_RS29530 (ACFJI2_29530) - 6605818..6606816 (+) 999 WP_107870017.1 KpsF/GutQ family sugar-phosphate isomerase -
  ACFJI2_RS29535 (ACFJI2_29535) - 6606816..6607355 (+) 540 WP_377703006.1 KdsC family phosphatase -
  ACFJI2_RS29540 (ACFJI2_29540) lptC 6607370..6607987 (+) 618 WP_377703007.1 LPS export ABC transporter periplasmic protein LptC -
  ACFJI2_RS29545 (ACFJI2_29545) lptA 6607991..6608566 (+) 576 WP_377703008.1 lipopolysaccharide transport periplasmic protein LptA -
  ACFJI2_RS29550 (ACFJI2_29550) lptB 6608569..6609348 (+) 780 WP_377703009.1 LPS export ABC transporter ATP-binding protein -
  ACFJI2_RS29555 (ACFJI2_29555) rpoN/rpoN1 6609355..6610845 (+) 1491 WP_377703010.1 RNA polymerase factor sigma-54 Machinery gene
  ACFJI2_RS29560 (ACFJI2_29560) hpf 6610915..6611271 (+) 357 WP_107870022.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  ACFJI2_RS29565 (ACFJI2_29565) - 6611365..6612441 (+) 1077 WP_377703011.1 enoyl-CoA hydratase/isomerase family protein -
  ACFJI2_RS29570 (ACFJI2_29570) rapZ 6612546..6613409 (-) 864 WP_377703012.1 RNase adapter RapZ -
  ACFJI2_RS29575 (ACFJI2_29575) hprK 6613518..6614453 (-) 936 WP_056404135.1 HPr(Ser) kinase/phosphatase -
  ACFJI2_RS29580 (ACFJI2_29580) - 6614498..6614980 (-) 483 WP_377703013.1 PTS sugar transporter subunit IIA -

Sequence

Protein


Download         Length: 496 a.a.        Molecular weight: 54893.43 Da        Isoelectric Point: 4.9215

>NTDB_id=1057925 ACFJI2_RS29555 WP_377703010.1 6609355..6610845(+) (rpoN/rpoN1) [Pseudoduganella sp. UC29_71]
MKQSLQLRTSQHLALTPQLQQSIRLLQLSTLELHQELEQLLGENPLLERLDDPLDRSLRLLSDGAINQTQPNGADASPAE
VGAPAQDSAPAAEGDPFEGATGEGDSASGAEADTDWSDAGRSKAPDDDDARPQLEASHCTLREHLMEQMRVTVQEPRDRV
LMELIIDALDENGYLEEPLEEIHARLPQEADVELEELQTALTLLQSFDPIGVGARSASECLALQIKRMPGVPLVTRRMAV
CIVEKHLAWFAQREFNKLKKALDCDDEDLREAQAVIRQCNPHPGAEFASDVSDYVVPDVIVKKARNGWQVTLNNDVMPRL
RVNALYANMLKQGKGETAMGAQLQEAKWLIKNMRQRFDTILRVAQAIVERQKNFFSHGAVAMRPLVLREIADTLGLHEST
ISRVTTQKYMLTPHGMFELKYFFGSHVATEAGGEASSTAIRALIVQLTGAEDPKNPLSDSKIADMLGEQGMVIARRTVAK
YREALKIPPVSLRKAL

Nucleotide


Download         Length: 1491 bp        

>NTDB_id=1057925 ACFJI2_RS29555 WP_377703010.1 6609355..6610845(+) (rpoN/rpoN1) [Pseudoduganella sp. UC29_71]
ATGAAACAGTCACTGCAACTCCGCACCTCTCAGCACCTGGCGCTGACGCCGCAGCTGCAGCAATCGATCCGGTTGCTGCA
GTTGTCCACGCTGGAGCTGCACCAGGAACTGGAACAGCTGCTGGGCGAGAACCCCTTGCTGGAGCGGCTGGACGACCCGC
TGGACCGCTCGCTGCGCCTGCTGAGCGACGGCGCCATCAACCAGACCCAGCCTAACGGCGCGGACGCTTCTCCGGCCGAG
GTCGGCGCGCCCGCGCAGGACAGCGCCCCGGCCGCCGAGGGCGATCCCTTCGAGGGCGCCACCGGCGAAGGCGACAGCGC
CAGCGGCGCCGAAGCCGACACCGACTGGAGCGACGCCGGCCGCAGCAAGGCGCCGGACGACGACGATGCGCGCCCGCAGC
TCGAAGCGAGCCACTGCACCTTGCGCGAACACCTGATGGAGCAGATGCGCGTGACCGTGCAGGAGCCGCGCGACCGCGTG
CTGATGGAACTGATCATCGACGCGCTGGACGAGAACGGCTACCTGGAAGAGCCGCTGGAGGAAATCCACGCGCGCCTGCC
GCAAGAGGCGGACGTGGAGCTGGAAGAACTGCAGACGGCGCTGACCCTGCTGCAAAGTTTCGACCCCATCGGCGTGGGCG
CGCGCAGCGCCTCCGAGTGCCTGGCGCTGCAGATCAAGCGCATGCCCGGCGTGCCCTTGGTGACGCGCCGCATGGCTGTC
TGCATCGTGGAAAAGCACCTGGCCTGGTTCGCGCAGCGCGAGTTCAACAAGCTGAAAAAAGCCCTCGATTGCGACGACGA
AGACTTGCGCGAAGCGCAGGCGGTGATCCGCCAGTGCAATCCGCATCCGGGCGCCGAGTTCGCTTCCGACGTGTCCGACT
ACGTGGTGCCGGACGTGATCGTGAAGAAGGCGCGCAATGGCTGGCAGGTCACGCTGAACAACGACGTGATGCCGCGCCTG
CGCGTGAACGCGCTGTACGCCAACATGCTCAAGCAGGGCAAAGGCGAAACCGCGATGGGTGCGCAGCTGCAGGAAGCGAA
GTGGCTGATCAAGAATATGCGCCAGCGTTTCGACACGATACTGCGCGTCGCACAAGCGATAGTAGAAAGACAAAAGAACT
TTTTCAGCCATGGCGCGGTTGCCATGAGACCCCTTGTGTTGCGTGAAATTGCTGATACACTGGGACTACACGAGAGCACT
ATTTCTCGCGTAACAACTCAGAAATACATGCTGACTCCGCATGGGATGTTTGAGTTGAAATATTTCTTTGGCAGTCACGT
CGCCACCGAAGCAGGAGGAGAAGCATCCTCGACGGCGATACGGGCGCTGATTGTGCAATTGACAGGAGCTGAAGACCCGA
AAAATCCTTTATCCGACAGTAAGATTGCGGACATGCTGGGTGAACAGGGCATGGTAATTGCGCGACGGACTGTTGCCAAA
TATCGCGAAGCCTTGAAAATCCCTCCGGTAAGTCTGCGTAAAGCTTTGTAG

Domains

Predicted by InterproScan.

Sigma-54 factor, core binding domain (Sigma54_CBD)

(135-326)

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(4-48)

Sigma-54, DNA binding domain (Sigma54_DBD)

(340-494)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

60.158

100

0.615

Multiple sequence alignment