Detailed information    

insolico Bioinformatically predicted

Overview

Name   rpoN/rpoN1   Type   Machinery gene
Locus tag   ACFC2F_RS16230 Genome accession   NZ_CP170190
Coordinates   3387367..3388800 (-) Length   477 a.a.
NCBI ID   WP_025756691.1    Uniprot ID   A0A0F1BDQ8
Organism   Enterobacter sichuanensis strain B36     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3382367..3393800
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACFC2F_RS16200 (ACFC2F_16200) elbB 3383899..3384552 (+) 654 WP_025756688.1 isoprenoid biosynthesis glyoxalase ElbB -
  ACFC2F_RS16205 (ACFC2F_16205) mtgA 3384549..3385274 (+) 726 WP_095441913.1 monofunctional biosynthetic peptidoglycan transglycosylase -
  ACFC2F_RS16210 (ACFC2F_16210) npr 3385314..3385586 (-) 273 WP_025756690.1 PTS phosphocarrier protein NPr -
  ACFC2F_RS16215 (ACFC2F_16215) rapZ 3385583..3386437 (-) 855 WP_014885397.1 RNase adapter RapZ -
  ACFC2F_RS16220 (ACFC2F_16220) ptsN 3386483..3386974 (-) 492 WP_008501443.1 PTS IIA-like nitrogen regulatory protein PtsN -
  ACFC2F_RS16225 (ACFC2F_16225) hpf 3387057..3387344 (-) 288 WP_013098943.1 ribosome hibernation promoting factor -
  ACFC2F_RS16230 (ACFC2F_16230) rpoN/rpoN1 3387367..3388800 (-) 1434 WP_025756691.1 RNA polymerase factor sigma-54 Machinery gene
  ACFC2F_RS16235 (ACFC2F_16235) lptB 3388848..3389573 (-) 726 WP_025756692.1 LPS export ABC transporter ATP-binding protein -
  ACFC2F_RS16240 (ACFC2F_16240) lptA 3389580..3390134 (-) 555 WP_025756693.1 lipopolysaccharide ABC transporter substrate-binding protein LptA -
  ACFC2F_RS16245 (ACFC2F_16245) lptC 3390103..3390678 (-) 576 WP_047959546.1 LPS export ABC transporter periplasmic protein LptC -
  ACFC2F_RS16250 (ACFC2F_16250) kdsC 3390675..3391241 (-) 567 WP_059385405.1 3-deoxy-manno-octulosonate-8-phosphatase KdsC -
  ACFC2F_RS16255 (ACFC2F_16255) kdsD 3391256..3392242 (-) 987 WP_059385406.1 arabinose-5-phosphate isomerase KdsD -
  ACFC2F_RS16260 (ACFC2F_16260) - 3392256..3393233 (-) 978 WP_045284743.1 calcium/sodium antiporter -

Sequence

Protein


Download         Length: 477 a.a.        Molecular weight: 54195.98 Da        Isoelectric Point: 4.3358

>NTDB_id=1055479 ACFC2F_RS16230 WP_025756691.1 3387367..3388800(-) (rpoN/rpoN1) [Enterobacter sichuanensis strain B36]
MKQGLQLRLSQQLAMTPQLQQAIRLLQLSTLELQQELQQALDSNPLLEQTDLHDEVDTQQSQDTEALDTADALEQKEMPD
ELPLDASWDEIYTAGTPSGTRADYQDDELPVYQGETTQSLQDYLMWQVELTPFSDTDRAIATSIVDAVDDTGYLTVTLDD
ILESMGDEEIELEEVEAVLKRIQRFDPVGVAARDLRDCLLIQLSQFSKETPWLDEARLIISDHLDLLANHDFRTLMRVTR
LKEDVLKEAVNLIQSLDPRPGQSIQTSEPEYVIPDVLVRKHNGRWVVELNSDSIPRLQINQQYASMCTSVRNDSDNQYIR
SNLQEARWLIKSLESRNDTLLRVSRCIVEQQQAFFEQGEEYMKPMVLADIAQAVEMHESTISRVTTQKYLHSPRGIFELK
YFFSSHVNTEGGGEASSTAIRALVKKLIAAENPAKPLSDSKLTTMLSEQGIMVARRTVAKYRESLSIPPSNQRKQLV

Nucleotide


Download         Length: 1434 bp        

>NTDB_id=1055479 ACFC2F_RS16230 WP_025756691.1 3387367..3388800(-) (rpoN/rpoN1) [Enterobacter sichuanensis strain B36]
ATGAAGCAAGGTTTGCAATTAAGGCTCAGCCAACAACTGGCAATGACGCCGCAGCTACAGCAGGCAATTCGCCTGTTGCA
ACTGTCCACGTTAGAACTTCAGCAGGAACTCCAGCAGGCGCTGGACAGTAACCCCCTGCTGGAACAAACCGATCTTCATG
ACGAGGTAGATACCCAGCAATCGCAGGATACAGAAGCGCTCGACACCGCGGATGCACTCGAACAAAAAGAGATGCCGGAC
GAGCTTCCGCTGGATGCCAGCTGGGATGAGATCTACACCGCCGGAACCCCATCCGGAACGCGTGCGGACTACCAGGACGA
TGAGCTACCGGTCTATCAGGGAGAAACCACTCAGTCGCTTCAGGATTACCTGATGTGGCAGGTGGAACTGACCCCCTTCT
CCGACACCGATCGTGCGATTGCCACATCCATTGTCGATGCCGTTGATGACACCGGCTATTTAACCGTCACGCTGGACGAC
ATCCTCGAAAGCATGGGCGACGAAGAGATTGAGCTTGAGGAAGTTGAAGCCGTTCTGAAGCGCATTCAGCGTTTCGATCC
GGTAGGCGTGGCCGCAAGAGATCTGCGCGACTGCCTGCTGATCCAGCTTTCTCAGTTCAGCAAAGAGACGCCGTGGCTTG
ATGAGGCGCGCTTAATCATCAGCGATCATCTGGATCTGTTGGCTAACCACGATTTCCGCACCCTGATGCGCGTCACGCGG
CTGAAAGAAGACGTGCTGAAAGAAGCGGTGAATCTGATACAGTCGCTCGATCCGCGCCCCGGTCAGTCGATCCAGACCAG
CGAACCCGAATACGTGATCCCGGATGTGCTGGTCCGAAAACACAATGGCCGCTGGGTCGTTGAACTGAATTCTGACAGCA
TTCCTCGCCTGCAAATCAACCAGCAGTATGCCTCCATGTGCACCAGCGTGCGTAACGACTCCGACAATCAATATATTCGT
AGCAATTTGCAGGAAGCGCGATGGTTGATCAAAAGTCTGGAGAGCCGTAATGATACGCTGCTTCGCGTCAGCCGCTGCAT
TGTCGAACAGCAGCAGGCGTTCTTTGAGCAGGGCGAAGAGTATATGAAACCGATGGTGCTGGCGGATATCGCCCAAGCCG
TCGAAATGCATGAATCAACCATTTCCCGTGTCACCACGCAGAAGTATCTGCACAGTCCACGCGGTATTTTTGAGCTTAAG
TATTTCTTCTCCAGCCACGTCAATACCGAGGGTGGCGGCGAAGCGTCGTCAACCGCGATTCGTGCCCTGGTGAAGAAGTT
GATCGCCGCGGAGAACCCCGCGAAGCCACTGAGTGACAGTAAGTTAACCACCATGCTGTCCGAACAGGGTATTATGGTGG
CACGTCGTACTGTCGCGAAGTATCGAGAGTCTTTATCCATTCCGCCGTCCAACCAGCGTAAACAGCTGGTCTGA

Domains

Predicted by InterproScan.

Sigma-54 factor, Activator interacting domain (AID) (Sigma54_AID)

(5-48)

Sigma-54 factor, core binding domain (Sigma54_CBD)

(116-303)

Sigma-54, DNA binding domain (Sigma54_DBD)

(317-475)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F1BDQ8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  rpoN/rpoN1 Ralstonia pseudosolanacearum GMI1000

45.941

100

0.486

Multiple sequence alignment