Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGF   Type   Machinery gene
Locus tag   ACEWY1_RS06615 Genome accession   NZ_CP169266
Coordinates   1297575..1298072 (+) Length   165 a.a.
NCBI ID   WP_001789864.1    Uniprot ID   -
Organism   Staphylococcus aureus strain CBC235     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1292575..1303072
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACEWY1_RS06575 (ACEWY1_06575) - 1292619..1293605 (+) 987 WP_000161314.1 ROK family glucokinase -
  ACEWY1_RS06580 (ACEWY1_06580) - 1293605..1293934 (+) 330 WP_001018871.1 MTH1187 family thiamine-binding protein -
  ACEWY1_RS06585 (ACEWY1_06585) - 1293931..1294554 (+) 624 WP_001223008.1 MBL fold metallo-hydrolase -
  ACEWY1_RS06590 (ACEWY1_06590) comGA 1294606..1295580 (+) 975 WP_000697228.1 competence type IV pilus ATPase ComGA Machinery gene
  ACEWY1_RS06595 (ACEWY1_06595) comGB 1295552..1296622 (+) 1071 Protein_1261 competence type IV pilus assembly protein ComGB -
  ACEWY1_RS06600 (ACEWY1_06600) comGC 1296636..1296947 (+) 312 WP_000472256.1 competence type IV pilus major pilin ComGC Machinery gene
  ACEWY1_RS06605 (ACEWY1_06605) comGD 1296925..1297371 (+) 447 WP_001790850.1 competence type IV pilus minor pilin ComGD Machinery gene
  ACEWY1_RS06610 (ACEWY1_06610) comGE 1297358..1297657 (+) 300 WP_000844413.1 hypothetical protein Machinery gene
  ACEWY1_RS06615 (ACEWY1_06615) comGF 1297575..1298072 (+) 498 WP_001789864.1 competence type IV pilus minor pilin ComGF Machinery gene
  ACEWY1_RS06620 (ACEWY1_06620) - 1298169..1298315 (+) 147 WP_001789879.1 hypothetical protein -
  ACEWY1_RS06625 (ACEWY1_06625) - 1298305..1298829 (+) 525 WP_001015117.1 shikimate kinase -
  ACEWY1_RS06630 (ACEWY1_06630) gcvT 1298988..1300079 (+) 1092 WP_000093349.1 glycine cleavage system aminomethyltransferase GcvT -
  ACEWY1_RS06635 (ACEWY1_06635) gcvPA 1300099..1301445 (+) 1347 WP_048665334.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPA -
  ACEWY1_RS06640 (ACEWY1_06640) gcvPB 1301438..1302910 (+) 1473 WP_000202189.1 aminomethyl-transferring glycine dehydrogenase subunit GcvPB -

Sequence

Protein


Download         Length: 165 a.a.        Molecular weight: 19164.88 Da        Isoelectric Point: 10.2192

>NTDB_id=1050124 ACEWY1_RS06615 WP_001789864.1 1297575..1298072(+) (comGF) [Staphylococcus aureus strain CBC235]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRDLTTVDFEFFSRDI
LDDFKGVDRNDIEIRQHRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNVQT
KTIYV

Nucleotide


Download         Length: 498 bp        

>NTDB_id=1050124 ACEWY1_RS06615 WP_001789864.1 1297575..1298072(+) (comGF) [Staphylococcus aureus strain CBC235]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTCATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGTAAAACTTTTCTAATTGAAAGTAGGGATTTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGATGATTTTAAAGGAGTAGATAGAAACGATATTGAAATTAGGCAACACCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATTAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCCATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATGTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Putative Competence protein ComGF (ComGF)

(67-155)

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGF Staphylococcus aureus MW2

100

100

1
  comGF Staphylococcus aureus N315

96.364

100

0.964

Multiple sequence alignment