Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilT   Type   Machinery gene
Locus tag   ABQY64_RS14395 Genome accession   NZ_CP168194
Coordinates   3430327..3431364 (-) Length   345 a.a.
NCBI ID   WP_003489919.1    Uniprot ID   -
Organism   Xanthomonas sp. WHRI 7065     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3425327..3436364
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ABQY64_RS14370 (ABQY64_014370) ruvX 3426075..3426542 (-) 468 WP_104573639.1 Holliday junction resolvase RuvX -
  ABQY64_RS14375 (ABQY64_014375) - 3426535..3427101 (-) 567 WP_011051924.1 YqgE/AlgH family protein -
  ABQY64_RS14380 (ABQY64_014380) - 3427436..3427981 (+) 546 WP_349779825.1 DNA-3-methyladenine glycosylase I -
  ABQY64_RS14385 (ABQY64_014385) - 3428118..3428792 (-) 675 WP_349779927.1 YitT family protein -
  ABQY64_RS14390 (ABQY64_014390) pilU 3429074..3430204 (-) 1131 WP_016902355.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  ABQY64_RS14395 (ABQY64_014395) pilT 3430327..3431364 (-) 1038 WP_003489919.1 type IV pilus twitching motility protein PilT Machinery gene
  ABQY64_RS14400 (ABQY64_014400) - 3431688..3432380 (+) 693 WP_349779826.1 YggS family pyridoxal phosphate-dependent enzyme -
  ABQY64_RS14405 (ABQY64_014405) proC 3432442..3433290 (+) 849 WP_349779827.1 pyrroline-5-carboxylate reductase -
  ABQY64_RS14410 (ABQY64_014410) - 3433891..3434316 (+) 426 WP_026064288.1 HU family DNA-binding protein -
  ABQY64_RS14415 (ABQY64_014415) - 3434453..3435073 (+) 621 WP_039526211.1 M23 family metallopeptidase -
  ABQY64_RS14420 (ABQY64_014420) - 3435166..3435474 (+) 309 WP_016902350.1 DUF3861 family protein -
  ABQY64_RS14425 (ABQY64_014425) - 3435550..3436074 (+) 525 WP_374151367.1 hypothetical protein -

Sequence

Protein


Download         Length: 345 a.a.        Molecular weight: 38643.30 Da        Isoelectric Point: 6.6652

>NTDB_id=1042991 ABQY64_RS14395 WP_003489919.1 3430327..3431364(-) (pilT) [Xanthomonas sp. WHRI 7065]
MDIAELLAFSVKNKASDLHLSAGLPPMIRVDGDVRRINIPALDHKQVHALVYDIMSDKQRRDYEEFLEVDFSFEIPSLAR
FRVNAFNQNRGAGAVFRTIPSEVLTLEDLGCPPIFRQLIDQPQGLILVTGPTGSGKSTTLAGMIDYINKNEYGHILTVED
PIEFVHTSQKCLINQREVHRDTHGFNEALRSALREDPDIILVGELRDLETIRLALTAAETGHLVFGTLHTSSAAKTIDRI
IDVFPAGEKPMVRSMLSESLRAVISQALLKKVGGGRTAAWEIMVGTPAIRNLIREDKVAQMYSSIQTGQQYGMQTLDQHL
QDLVKRSLITRNQAREYAKDKRIFE

Nucleotide


Download         Length: 1038 bp        

>NTDB_id=1042991 ABQY64_RS14395 WP_003489919.1 3430327..3431364(-) (pilT) [Xanthomonas sp. WHRI 7065]
ATGGATATCGCTGAACTATTGGCGTTTTCCGTCAAAAACAAGGCATCGGACCTGCATCTGTCGGCCGGCTTGCCGCCGAT
GATCCGTGTCGATGGCGATGTCCGTCGCATCAATATTCCGGCCCTGGATCACAAGCAGGTGCATGCGCTGGTCTACGACA
TCATGTCGGACAAGCAGCGTCGCGATTACGAGGAATTCCTCGAGGTCGACTTCTCGTTCGAGATTCCGTCGCTGGCGCGC
TTCCGCGTCAACGCGTTCAACCAGAACCGCGGCGCCGGTGCGGTGTTCCGCACCATTCCTTCCGAAGTGCTGACGCTGGA
GGACCTGGGCTGCCCGCCGATCTTCCGCCAGTTGATCGACCAGCCGCAGGGTCTGATCCTGGTCACCGGTCCGACCGGTT
CGGGCAAGTCGACCACGCTCGCCGGCATGATCGACTACATCAACAAGAACGAATACGGCCACATCCTCACGGTCGAGGAT
CCGATCGAATTCGTGCATACCTCGCAGAAGTGCCTGATCAATCAGCGCGAAGTGCACCGCGATACGCACGGCTTCAACGA
GGCGCTGCGCTCGGCGCTGCGCGAAGATCCGGACATCATCCTTGTCGGCGAATTGCGCGACCTGGAAACCATCCGCCTGG
CGCTGACCGCCGCCGAAACCGGCCACCTGGTGTTCGGCACCCTGCACACCAGCTCGGCGGCCAAGACCATCGACCGCATC
ATCGACGTGTTCCCGGCCGGCGAAAAGCCGATGGTGCGCTCGATGCTGTCCGAGTCGCTGCGTGCGGTGATTTCGCAGGC
GCTGCTGAAGAAGGTCGGCGGCGGACGCACCGCGGCCTGGGAAATCATGGTCGGCACCCCGGCCATCCGCAACCTGATCC
GCGAGGACAAGGTGGCGCAGATGTATTCCTCGATCCAGACCGGCCAGCAATACGGTATGCAGACGCTGGACCAGCATCTG
CAGGACCTGGTCAAGCGCAGCCTGATCACGCGCAACCAGGCACGCGAGTACGCCAAGGACAAGCGGATATTCGAGTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(11-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilT Acinetobacter nosocomialis M2

79.942

99.71

0.797
  pilT Acinetobacter baumannii D1279779

79.942

99.71

0.797
  pilT Acinetobacter baumannii strain A118

79.942

99.71

0.797
  pilT Pseudomonas stutzeri DSM 10701

79.36

99.71

0.791
  pilT Acinetobacter baylyi ADP1

78.779

99.71

0.786
  pilT Pseudomonas aeruginosa PAK

79.056

98.261

0.777
  pilT Legionella pneumophila strain Lp02

75.291

99.71

0.751
  pilT Legionella pneumophila strain ERS1305867

75.291

99.71

0.751
  pilT Vibrio cholerae O1 biovar El Tor strain E7946

71.125

95.362

0.678
  pilT Vibrio cholerae strain A1552

71.125

95.362

0.678
  pilT Neisseria meningitidis 8013

65.982

98.841

0.652
  pilT Neisseria gonorrhoeae MS11

65.689

98.841

0.649
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

49.855

100

0.499
  pilU Pseudomonas stutzeri DSM 10701

40.708

98.261

0.4
  pilU Acinetobacter baylyi ADP1

40.294

98.551

0.397
  pilU Vibrio cholerae strain A1552

40.597

97.101

0.394

Multiple sequence alignment