Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   ACDZ29_RS00900 Genome accession   NZ_CP168135
Coordinates   161103..162179 (-) Length   358 a.a.
NCBI ID   WP_375091079.1    Uniprot ID   -
Organism   Peribacillus sp. RS7     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 156103..167179
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACDZ29_RS00860 (ACDZ29_00855) - 156949..157746 (+) 798 WP_289325719.1 YqhG family protein -
  ACDZ29_RS00865 (ACDZ29_00860) - 157792..157977 (-) 186 WP_289315683.1 YqzE family protein -
  ACDZ29_RS00870 (ACDZ29_00865) comGG 158105..158491 (-) 387 WP_375089514.1 competence type IV pilus minor pilin ComGG -
  ACDZ29_RS00875 (ACDZ29_00870) comGF 158484..158963 (-) 480 WP_289334753.1 competence type IV pilus minor pilin ComGF -
  ACDZ29_RS00880 (ACDZ29_00875) - 158911..159249 (-) 339 WP_375089516.1 hypothetical protein -
  ACDZ29_RS00885 (ACDZ29_00880) comGD 159236..159694 (-) 459 WP_289315679.1 competence type IV pilus minor pilin ComGD -
  ACDZ29_RS00890 (ACDZ29_00885) comGC 159687..159995 (-) 309 WP_375091078.1 competence type IV pilus major pilin ComGC Machinery gene
  ACDZ29_RS00895 (ACDZ29_00890) comGB 160085..161137 (-) 1053 WP_289334751.1 competence type IV pilus assembly protein ComGB -
  ACDZ29_RS00900 (ACDZ29_00895) comGA 161103..162179 (-) 1077 WP_375091079.1 competence type IV pilus ATPase ComGA Machinery gene
  ACDZ29_RS00905 (ACDZ29_00900) - 162286..162666 (-) 381 WP_370015789.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  ACDZ29_RS00910 (ACDZ29_00905) - 162881..163582 (+) 702 WP_375089518.1 helix-turn-helix transcriptional regulator -
  ACDZ29_RS00915 (ACDZ29_00910) - 163668..163910 (+) 243 WP_289315675.1 DUF2626 domain-containing protein -
  ACDZ29_RS00920 (ACDZ29_00915) - 163966..165063 (-) 1098 WP_375089519.1 class I SAM-dependent methyltransferase -
  ACDZ29_RS00925 (ACDZ29_00920) - 165500..166126 (-) 627 WP_375089520.1 MBL fold metallo-hydrolase -
  ACDZ29_RS00930 (ACDZ29_00925) - 166360..166533 (+) 174 WP_289315672.1 DUF2759 domain-containing protein -

Sequence

Protein


Download         Length: 358 a.a.        Molecular weight: 39923.52 Da        Isoelectric Point: 8.1644

>NTDB_id=1042441 ACDZ29_RS00900 WP_375091079.1 161103..162179(-) (comGA) [Peribacillus sp. RS7]
MISIEKTAEKILTRAIKLSASDIHIFFRREGPLIQFRIDNKLVPQETLSFFEAERLIAHLKFLAAMDIGEKRRPQSGAIT
INLANQVVGLRLSTLPTSYLESLVIRLIPQQNILPLEQLSLFPNTVQKLIALLKHSHGMLIFTGPTGSGKTTTLYSLLHH
DHEMINRNIITLEDPIENVSEKVLQVQINEKAGITYSVGLKAVLRHDPDVIMVGEVRDAETAKIAVRAALTGHLILTTMH
TRDAQGAISRLLEFGVSLLEIEQSLIGVTAQRLVELRCLPCNGDCAFACQMTARNKRASVYELLYGKSLAEVLRMMGDEK
GKATASYRLLKDEIGKAVAMGYVDSQEYERLVYHEAKN

Nucleotide


Download         Length: 1077 bp        

>NTDB_id=1042441 ACDZ29_RS00900 WP_375091079.1 161103..162179(-) (comGA) [Peribacillus sp. RS7]
TTGATATCGATTGAAAAGACCGCAGAAAAAATACTTACCCGTGCCATAAAGTTATCAGCATCGGATATCCACATTTTTTT
TCGCAGGGAGGGGCCTCTCATCCAATTCAGGATAGATAATAAACTCGTTCCCCAGGAAACATTATCATTCTTTGAAGCTG
AAAGGCTGATAGCCCATTTGAAGTTCCTTGCCGCAATGGACATAGGGGAGAAAAGGAGACCCCAGAGTGGTGCCATCACC
ATCAATTTGGCTAACCAAGTGGTTGGACTCCGCCTTTCCACTCTACCCACTTCCTATCTCGAAAGTTTGGTCATCCGCTT
AATACCCCAACAGAATATCCTTCCTCTAGAACAGTTATCCTTATTTCCAAACACCGTTCAAAAATTGATTGCACTTTTGA
AGCATTCCCATGGCATGCTCATTTTTACCGGTCCGACCGGCAGTGGGAAAACCACGACACTATATTCCCTGCTTCATCAT
GATCATGAGATGATCAATCGAAATATCATAACGCTTGAAGATCCCATTGAAAATGTATCTGAAAAGGTACTGCAAGTCCA
AATCAATGAAAAGGCGGGCATTACTTATTCTGTCGGACTTAAAGCTGTGCTAAGGCATGATCCCGACGTGATCATGGTAG
GGGAAGTCAGGGATGCCGAAACGGCCAAAATCGCAGTGCGTGCAGCTCTAACCGGCCATTTGATACTTACAACCATGCAT
ACAAGGGATGCACAGGGTGCTATCTCCAGGTTACTTGAATTTGGTGTCAGCTTGCTTGAGATTGAACAGAGTTTGATTGG
CGTGACAGCACAGCGGCTGGTTGAATTGAGGTGTCTTCCATGTAATGGGGACTGTGCTTTTGCTTGCCAAATGACTGCCA
GGAATAAAAGGGCAAGTGTGTATGAATTGCTTTATGGAAAAAGCCTGGCCGAGGTTCTCCGGATGATGGGGGATGAAAAA
GGAAAGGCAACGGCCAGCTACCGCCTATTGAAGGATGAAATAGGAAAAGCTGTTGCGATGGGCTATGTGGATTCGCAGGA
ATATGAACGGCTTGTATACCATGAAGCTAAAAACTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

56.303

99.721

0.561
  pilB Haemophilus influenzae 86-028NP

39.08

97.207

0.38
  pilB Haemophilus influenzae Rd KW20

37.931

97.207

0.369

Multiple sequence alignment