Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   ACBZ91_RS11255 Genome accession   NZ_CP167179
Coordinates   2405335..2405910 (-) Length   191 a.a.
NCBI ID   WP_243975175.1    Uniprot ID   -
Organism   Vibrio natriegens strain JSH01     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2400335..2410910
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ACBZ91_RS11230 (ACBZ91_11230) aroB 2400634..2401734 (-) 1101 WP_014233146.1 3-dehydroquinate synthase -
  ACBZ91_RS11235 (ACBZ91_11235) aroK 2401766..2402284 (-) 519 WP_014233147.1 shikimate kinase AroK -
  ACBZ91_RS11240 (ACBZ91_11240) pilQ 2402473..2404176 (-) 1704 WP_372378691.1 type IV pilus secretin PilQ Machinery gene
  ACBZ91_RS11245 (ACBZ91_11245) pilP 2404244..2404759 (-) 516 WP_020334614.1 pilus assembly protein PilP Machinery gene
  ACBZ91_RS11250 (ACBZ91_11250) pilO 2404749..2405342 (-) 594 WP_372377396.1 type 4a pilus biogenesis protein PilO Machinery gene
  ACBZ91_RS11255 (ACBZ91_11255) pilN 2405335..2405910 (-) 576 WP_243975175.1 PilN domain-containing protein Machinery gene
  ACBZ91_RS11260 (ACBZ91_11260) pilM 2405894..2406910 (-) 1017 WP_372377397.1 type IV pilus assembly protein PilM Machinery gene
  ACBZ91_RS11265 (ACBZ91_11265) - 2407054..2409621 (+) 2568 WP_372377398.1 penicillin-binding protein 1A -
  ACBZ91_RS11270 (ACBZ91_11270) oxyR 2409685..2410593 (-) 909 WP_014233154.1 DNA-binding transcriptional regulator OxyR -

Sequence

Protein


Download         Length: 191 a.a.        Molecular weight: 22375.73 Da        Isoelectric Point: 5.9580

>NTDB_id=1038696 ACBZ91_RS11255 WP_243975175.1 2405335..2405910(-) (pilN) [Vibrio natriegens strain JSH01]
MLYRINLLPWRENQREEHRRRFVGMFILGLFCAVGIQWGIGKFYEIQQDQQQDRLDYLTHYITELDQRIEAMKIAEQEHS
KILERLKVVEGLQNGRNKTTEFMNLMPAVIPEGVYVDKIKMNDLEIEIAGISDSTPRLATMLDNMERSAKLLDVEMHSIV
HGKERFGKEFQTFKVSFIFKAVEQEEGGQHG

Nucleotide


Download         Length: 576 bp        

>NTDB_id=1038696 ACBZ91_RS11255 WP_243975175.1 2405335..2405910(-) (pilN) [Vibrio natriegens strain JSH01]
GTGCTTTATCGAATTAACTTATTGCCCTGGAGGGAGAACCAAAGGGAAGAACATCGCCGCCGTTTTGTTGGCATGTTCAT
TCTGGGCTTGTTTTGTGCTGTTGGTATTCAATGGGGGATTGGGAAGTTTTATGAGATCCAACAAGATCAGCAGCAAGATC
GGCTCGATTATCTTACTCACTATATTACGGAACTTGATCAACGTATTGAAGCAATGAAAATTGCCGAGCAAGAGCACAGC
AAAATATTAGAGCGTTTGAAGGTGGTCGAAGGCTTGCAAAATGGTCGCAACAAAACCACAGAGTTCATGAACCTGATGCC
TGCTGTTATTCCTGAAGGTGTTTACGTCGATAAGATCAAAATGAATGATCTGGAAATCGAAATCGCGGGGATTTCTGATA
GCACACCACGTTTGGCAACCATGCTCGATAACATGGAGCGTTCGGCAAAGCTATTGGACGTTGAAATGCACTCTATTGTC
CATGGCAAAGAACGTTTCGGTAAAGAATTTCAGACCTTCAAAGTGTCTTTCATATTTAAAGCGGTAGAGCAGGAAGAGGG
AGGTCAGCATGGTTAA

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(103-175)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Vibrio campbellii strain DS40M4

82.199

100

0.822
  pilN Vibrio cholerae strain A1552

59.375

100

0.597

Multiple sequence alignment