Detailed information NTG1010871

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: ABOE65_RS12230
• Genome accession: NZ_CP158358
• Coordinates: 2491582..2492196 (-)
• Length: 204 a.a.
• NCBI ID: WP_012118026.1
• Uniprot ID: A7Z6X3
• Organism: Bacillus velezensis strain HY23
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2486582..2497196

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
ABOE65_RS12200 (ABOE65_12190)	rpsT	2486885..2487151 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
ABOE65_RS12205 (ABOE65_12195)	holA	2487168..2488209 (-)	1042	Protein_2356	DNA polymerase III subunit delta	-
ABOE65_RS12210 (ABOE65_12200)	-	2488249..2488400 (-)	152	Protein_2357	hypothetical protein	-
ABOE65_RS12215 (ABOE65_12205)	-	2488441..2488575 (+)	135	WP_003152870.1	YqzM family protein	-
ABOE65_RS12220 (ABOE65_12210)	comEC	2488594..2490945 (-)	2352	WP_012118025.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
ABOE65_RS12225 (ABOE65_12215)	-	2490946..2491515 (-)	570	WP_003152868.1	ComE operon protein 2	-
ABOE65_RS12230 (ABOE65_12220)	comEA	2491582..2492196 (-)	615	WP_012118026.1	helix-hairpin-helix domain-containing protein	Machinery gene
ABOE65_RS12235 (ABOE65_12225)	comER	2492255..2493076 (+)	822	WP_012118027.1	late competence protein ComER	-
ABOE65_RS12240 (ABOE65_12230)	-	2493145..2493882 (-)	738	WP_012118028.1	class I SAM-dependent methyltransferase	-
ABOE65_RS12245 (ABOE65_12235)	rsfS	2493879..2494235 (-)	357	WP_007408260.1	ribosome silencing factor	-
ABOE65_RS12250 (ABOE65_12240)	yqeK	2494252..2494812 (-)	561	WP_012118029.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
ABOE65_RS12255 (ABOE65_12245)	-	2494802..2495371 (-)	570	WP_012118030.1	nicotinate-nucleotide adenylyltransferase	-
ABOE65_RS12260 (ABOE65_12250)	yhbY	2495382..2495672 (-)	291	WP_007408257.1	ribosome assembly RNA-binding protein YhbY	-
ABOE65_RS12265 (ABOE65_12255)	aroE	2495666..2496508 (-)	843	WP_012118031.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22195.40 Da
• Isoelectric Point: 6.4888

>NTDB_id=1010871 ABOE65_RS12230 WP_012118026.1 2491582..2492196(-) (comEA) [Bacillus velezensis strain HY23]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENRPKTAAGEKENAAVNINMASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=1010871 ABOE65_RS12230 WP_012118026.1 2491582..2492196(-) (comEA) [Bacillus velezensis strain HY23]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTCGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGGGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATATGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

3D structure

• AlphaFold DB: A7Z6X3

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363